Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1991 Sep;85(3):498–503. doi: 10.1111/j.1365-2249.1991.tb05756.x

Human colonic intra-epithelial lymphocytes are suppressor cells.

P Hoang 1, H R Dalton 1, D P Jewell 1
PMCID: PMC1535625  PMID: 1832599

Abstract

Human colonic intra-epithelial lymphocytes (IEL) suppress the proliferation of autologous lamina propria lymphocytes, but not autologous peripheral blood mononuclear cells, when stimulated with phytohaemagglutinin. This suppressor function is mediated by a CD8-dependent soluble factor and is not related to the expression of the gamma delta T cell receptor. These findings may be relevant to the induction of mucosal tolerance. However, there is no defect in suppressor activity of colonic IEL in inflammatory bowel disease.

Full text

PDF
498

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brandtzaeg P., Halstensen T. S., Kett K., Krajci P., Kvale D., Rognum T. O., Scott H., Sollid L. M. Immunobiology and immunopathology of human gut mucosa: humoral immunity and intraepithelial lymphocytes. Gastroenterology. 1989 Dec;97(6):1562–1584. doi: 10.1016/0016-5085(89)90406-x. [DOI] [PubMed] [Google Scholar]
  2. Brandtzaeg P., Sollid L. M., Bjerke K., Kett K., Kvale D., Rognum T. O., Scott H., Thrane P. S. Interactions of lymphoid cells with the epithelial environment. Monogr Allergy. 1988;24:51–59. [PubMed] [Google Scholar]
  3. Bull D. M., Bookman M. A. Isolation and functional characterization of human intestinal mucosal lymphoid cells. J Clin Invest. 1977 May;59(5):966–974. doi: 10.1172/JCI108719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cerf-Bensussan N., Guy-Grand D., Griscelli C. Intraepithelial lymphocytes of human gut: isolation, characterisation and study of natural killer activity. Gut. 1985 Jan;26(1):81–88. doi: 10.1136/gut.26.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cerf-Bensussan N., Jarry A., Brousse N., Lisowska-Grospierre B., Guy-Grand D., Griscelli C. A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes. Eur J Immunol. 1987 Sep;17(9):1279–1285. doi: 10.1002/eji.1830170910. [DOI] [PubMed] [Google Scholar]
  6. Chiba M., Bartnik W., ReMine S. G., Thayer W. R., Shorter R. G. Human colonic intraepithelial and lamina proprial lymphocytes: cytotoxicity in vitro and the potential effects of the isolation method on their functional properties. Gut. 1981 Mar;22(3):177–186. doi: 10.1136/gut.22.3.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ebert E. C. Intra-epithelial lymphocytes: interferon-gamma production and suppressor/cytotoxic activities. Clin Exp Immunol. 1990 Oct;82(1):81–85. doi: 10.1111/j.1365-2249.1990.tb05407.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ebert E. C., Roberts A. I., Brolin R. E., Raska K. Examination of the low proliferative capacity of human jejunal intraepithelial lymphocytes. Clin Exp Immunol. 1986 Jul;65(1):148–157. [PMC free article] [PubMed] [Google Scholar]
  9. Fujihashi K., Taguchi T., McGhee J. R., Eldridge J. H., Bruce M. G., Green D. R., Singh B., Kiyono H. Regulatory function for murine intraepithelial lymphocytes. Two subsets of CD3+, T cell receptor-1+ intraepithelial lymphocyte T cells abrogate oral tolerance. J Immunol. 1990 Oct 1;145(7):2010–2019. [PubMed] [Google Scholar]
  10. Greenwood J. H., Austin L. L., Dobbins W. O., 3rd In vitro characterization of human intestinal intraepithelial lymphocytes. Gastroenterology. 1983 Nov;85(5):1023–1035. [PubMed] [Google Scholar]
  11. Haynes B. F., Fauci A. S. Mechanisms of corticosteroid action on lymphocyte subpopulations. IV. Effects of in vitro hydrocortisone on naturally occuring and mitogen-induced suppressor cells in man. Cell Immunol. 1979 Apr;44(1):157–168. doi: 10.1016/0008-8749(79)90036-4. [DOI] [PubMed] [Google Scholar]
  12. Hirata I., Berrebi G., Austin L. L., Keren D. F., Dobbins W. O., 3rd Immunohistological characterization of intraepithelial and lamina propria lymphocytes in control ileum and colon and in inflammatory bowel disease. Dig Dis Sci. 1986 Jun;31(6):593–603. doi: 10.1007/BF01318690. [DOI] [PubMed] [Google Scholar]
  13. Mayer L., Shlien R. Evidence for function of Ia molecules on gut epithelial cells in man. J Exp Med. 1987 Nov 1;166(5):1471–1483. doi: 10.1084/jem.166.5.1471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mowat A. M. Human intraepithelial lymphocytes. Springer Semin Immunopathol. 1990;12(2-3):165–190. doi: 10.1007/BF00197504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mowat A. M., Tait R. C., MacKenzie S., Davies M. D., Parrott D. M. Analysis of natural killer effector and suppressor activity by intraepithelial lymphocytes from mouse small intestine. Clin Exp Immunol. 1983 Apr;52(1):191–198. [PMC free article] [PubMed] [Google Scholar]
  16. Quere P., Cooper M. D., Thorbecke G. J. Characterization of suppressor T cells for antibody production by chicken spleen cells. I. Antigen-induced suppressor cells are CT8+, TcR1+ (gamma delta) T cells. Immunology. 1990 Dec;71(4):517–522. [PMC free article] [PubMed] [Google Scholar]
  17. Selby W. S., Janossy G., Bofill M., Jewell D. P. Intestinal lymphocyte subpopulations in inflammatory bowel disease: an analysis by immunohistological and cell isolation techniques. Gut. 1984 Jan;25(1):32–40. doi: 10.1136/gut.25.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Senju M., Hulstaert F., Lowder J., Jewell D. P. Flow cytometric analysis of peripheral blood lymphocytes in ulcerative colitis and Crohn's disease. Gut. 1991 Jul;32(7):779–783. doi: 10.1136/gut.32.7.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Smith C. I., Svejgaard A. Concanavalin-A-induced suppressor lymphocytes in normal individuals. Scand J Immunol. 1981;13(5):483–492. doi: 10.1111/j.1365-3083.1981.tb00160.x. [DOI] [PubMed] [Google Scholar]
  20. Trejdosiewicz L. K., Malizia G., Badr-el-Din S., Smart C. J., Oakes D. J., Southgate J., Howdle P. D., Janossy G., Poulter L. W., Losowsky M. S. T cell and mononuclear phagocyte populations of the human small and large intestine. Adv Exp Med Biol. 1987;216A:465–473. doi: 10.1007/978-1-4684-5344-7_54. [DOI] [PubMed] [Google Scholar]
  21. Trejdosiewicz L. K., Smart C. J., Oakes D. J., Howdle P. D., Malizia G., Campana D., Boylston A. W. Expression of T-cell receptors TcR1 (gamma/delta) and TcR2 (alpha/beta) in the human intestinal mucosa. Immunology. 1989 Sep;68(1):7–12. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES