Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1983 Aug;53(2):413–422.

Lymphocyte enzyme activities in immunodeficiency syndromes with particular reference to common variable hypogammaglobulinaemia.

T Shah, A D Webster, T J Peters
PMCID: PMC1535693  PMID: 6309445

Abstract

Circulating non-T lymphocytes had higher activities of 5'nucleotidase (plasma membrane), neutral alpha-glucosidase (endoplasmic reticulum) and basal leucine amino-peptidase than did T lymphocytes. Activities of catalase (peroxisomes), malate dehydrogenase (mitochondria), lactate dehydrogenase (cytosol) and N-acetyl-beta-glucosaminidase, beta-glucuronidase and acid phosphatase (lysosomes), were similar in the lymphocyte subfractions. Lymphocyte 5'nucleotidase (plasma membrane) in patients with common variable hypogammaglobulinaemia is much lower than normal. However, the decrease is less marked in X-linked hypogammaglobulinaemia, chronic lymphatic leukaemia or protein loosing enteropathy or in lymphocytes isolated from cord blood. Cells from patients with nephrotic syndrome had normal levels of 5'nucleotidase. Other plasma membrane marker enzymes (gamma-glutamyl transferase, leucine amino-peptidase) were normal in lymphocytes from patients with common variable hypogammaglobulinaemia. There is a selective reduction of mitochondrial (malate dehydrogenase) and cytosolic (lactate dehydrogenase) enzymes, with normal activities of lysosomal, peroxisomal and endoplasmic reticulum enzymes, in patients with common variable hypogammaglobulinaemia. The lymphocyte subcellular organelles in normal subjects and patients with common variable hypogammaglobulinaemia have similar properties on sucrose density gradient centrifugation. It is suggested that lymphocytes from patients with common variable hypogammaglobulinaemia show a specific enzymopathy and that this is not simply a reflection of cellular immaturity.

Full text

PDF
413

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adebonojo F. O., Coates P. M., Cortner J. A. Acid and neutral lipases of cultured adipocytes of infants and children. Pediatr Res. 1978 Jan;12(1):27–29. doi: 10.1203/00006450-197801000-00007. [DOI] [PubMed] [Google Scholar]
  2. Crockard A. D., Lewis M. H., Bridges J. M. N-Acetyl-beta-D-glucosaminidase activity in normal and chronic lymphocytic leukaemic lymphocytes. Clin Chim Acta. 1979 Apr 2;93(1):151–156. doi: 10.1016/0009-8981(79)90256-0. [DOI] [PubMed] [Google Scholar]
  3. Edwards N. L., Magilavy D. B., Cassidy J. T., Fox I. H. Lymphocyte ecto-5'-nucleotidase deficiency in agammaglobulinemia. Science. 1978 Aug 18;201(4356):628–630. doi: 10.1126/science.27864. [DOI] [PubMed] [Google Scholar]
  4. Gmelig-Meyling F., Ballieux R. E. Simplified procedure for the separation of human T and non-T cells. Vox Sang. 1977 Jul;33(1):5–8. doi: 10.1111/j.1423-0410.1977.tb02229.x. [DOI] [PubMed] [Google Scholar]
  5. Johnson S. M., North M. E., Asherson G. L., Allsop J., Watts R. W., Webster A. D. Lymphocyte purine 5'-nucleotidase edficiency in primary hypogammaglobulinaemia. Lancet. 1977 Jan 22;1(8004):168–170. doi: 10.1016/s0140-6736(77)91765-2. [DOI] [PubMed] [Google Scholar]
  6. Kapuściński J., Skoczylas B. Simple and rapid fluorimetric method for DNA microassay. Anal Biochem. 1977 Nov;83(1):252–257. doi: 10.1016/0003-2697(77)90533-4. [DOI] [PubMed] [Google Scholar]
  7. Matamoros N., Abad E., Webster A. D. LDH isoenzymes in the T lymphocytes of patients with primary hypogammaglobulinaemia. Clin Exp Immunol. 1982 Dec;50(3):617–620. [PMC free article] [PubMed] [Google Scholar]
  8. Matamoros N., Horwitz D. A., Newton C., Asherson G. L., Webster A. D. Histochemical studies for 5'-nucleotidase and alpha-naphthyl (non-specific) esterase in lymphocytes from patients with primary immunoglobulin deficiencies. Clin Exp Immunol. 1979 Apr;36(1):102–106. [PMC free article] [PubMed] [Google Scholar]
  9. Mueller J., Brun del Re G., Buerki H., Keller H. U., Hess M. W., Cottier H. Nonspecific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes. Eur J Immunol. 1975 Apr;5(4):270–274. doi: 10.1002/eji.1830050411. [DOI] [PubMed] [Google Scholar]
  10. Peters T. J. Analytical subcellular fractionation of jejunal biopsy specimens: methodology and characterization of the organelles in normal tissue. Clin Sci Mol Med. 1976 Dec;51(6):557–574. doi: 10.1042/cs0510557. [DOI] [PubMed] [Google Scholar]
  11. Peters T. J., Seymour C. A. Analytical subcellular fractionation of needle-biopsy specimens from human liver. Biochem J. 1978 Aug 15;174(2):435–446. doi: 10.1042/bj1740435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Quagliata F., Faig D., Conklyn M., Silber R. Studies on the lymphocyte 5'-nucleotidase in chronic lymphocytic leukemia, infectious mononucleosis, normal subpopulations, and phytohemagglutinin-stimulated cells. Cancer Res. 1974 Dec;34(12):3197–3202. [PubMed] [Google Scholar]
  13. Rowe M., de Gast C. G., Platts-Mills T. A., Asherson G. L., Webster A. D., Johnson S. M. 5'-nucleotidase of B and T lymphocytes isolated from human peripheral blood. Clin Exp Immunol. 1979 Apr;36(1):97–101. [PMC free article] [PubMed] [Google Scholar]
  14. Rowe M., de Gast G. C., Platts-Mills T. A., Asherson G. L., Webster A. D., Johnson S. M. Lymphocyte 5'-nucleotidase in primary hypogammaglobulinaemia and cord blood. Clin Exp Immunol. 1980 Feb;39(2):337–343. [PMC free article] [PubMed] [Google Scholar]
  15. Rustin G. J., Peters T. J. Studies on the subcellular organelles of neutrophils in chronic granulocytic leukaemia with special reference to alkaline phosphatase. Br J Haematol. 1979 Apr;41(4):533–543. doi: 10.1111/j.1365-2141.1979.tb05891.x. [DOI] [PubMed] [Google Scholar]
  16. Schacterle G. R., Pollack R. L. A simplified method for the quantitative assay of small amounts of protein in biologic material. Anal Biochem. 1973 Feb;51(2):654–655. doi: 10.1016/0003-2697(73)90523-x. [DOI] [PubMed] [Google Scholar]
  17. Seymour C. A., Peters T. J. Enzyme activities in human liver biopsies: assay methods and activities of some lysosomal and membrane-bound enzymes in control tissue and serum. Clin Sci Mol Med. 1977 Mar;52(3):229–239. doi: 10.1042/cs0520229. [DOI] [PubMed] [Google Scholar]
  18. Smith G. P., Shah T., Webster A. D., Peters T. J. Studies on the kinetic properties and subcellular localization of adenine nucleotide phosphatases in peripheral blood lymphocytes from control subjects and patients with common variable primary hypogammaglobulinaemia. Clin Exp Immunol. 1982 Aug;49(2):393–400. [PMC free article] [PubMed] [Google Scholar]
  19. Tötterman T. H., Ranki A., Häyry P. Expression of the acid alpha-naphthyl acetate esterase marker by activated and secondary T lymphocytes in man. Scand J Immunol. 1977;6(4):305–310. doi: 10.1111/j.1365-3083.1977.tb00398.x. [DOI] [PubMed] [Google Scholar]
  20. WALDMANN T. A. Gastrointestinal protein loss demonstrated by Cr-51-labelled albumin. Lancet. 1961 Jul 15;2(7194):121–123. doi: 10.1016/s0140-6736(61)92646-0. [DOI] [PubMed] [Google Scholar]
  21. Webster A. D., North M., Allsop J., Asherson G. L., Watts R. W. Purine metabolism in lymphocytes from patients with primary hypogammaglobulinaemia. Clin Exp Immunol. 1978 Mar;31(3):456–463. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES