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. 1983 Aug;53(2):451–457.

Characteristics of suppressor factors generated from human mononuclear cells using concanavalin A and C-reactive protein.

A G Wangel, S Kontiainen
PMCID: PMC1535698  PMID: 6192956

Abstract

Lymphocytes from normal human donors were cultured in Marbrook flasks in the presence of C-reactive protein (CRP), concanavalin A (Con A) or serum amyloid A protein low molecular weight component (SAAL) and the culture supernatants were tested for their ability to suppress the pokeweed mitogen (PWM) driven response of normal human lymphocytes. The supernatants from CRP and Con A stimulated lymphocytes, when added to cultures at initiation at a final concentration of 5%, suppressed the plaque forming cell (PFC) response in the three major immunoglobulin (Ig) classes. The suppressive effect could be removed by absorption of the culture supernatants with lentil lectin and with antisera to antigen specific helper and suppressor factors and could be recovered in the eluate. The results indicate that CRP stimulated cells exert their suppressive effect through a soluble mediator(s) which is similar to Con A generated suppressor factor(s) in respect to its time of action, its effect on the Ig classes M, G and A, its lack of cytoxicity and some structural aspects.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldo-Benson M. A., Benson M. D. SAA suppression of immune response in vitro: evidence for an effect on T cell-macrophage interaction. J Immunol. 1982 Jun;128(6):2390–2392. [PubMed] [Google Scholar]
  2. Andersson J., Sjöberg O., Möller G. Induction of immunoglobulin and antibody synthesis in vitro by lipopolysaccharides. Eur J Immunol. 1972 Aug;2(4):349–353. doi: 10.1002/eji.1830020410. [DOI] [PubMed] [Google Scholar]
  3. Bausserman L. L., Herbert P. N., McAdam K. P. Heterogeneity of human serum amyloid A proteins. J Exp Med. 1980 Sep 1;152(3):641–656. doi: 10.1084/jem.152.3.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Biberfeld G., Gronowicz E. Mycoplasma pneumoniae is a polyclonal B-cell activator. Nature. 1976 May 20;261(5557):238–239. doi: 10.1038/261238a0. [DOI] [PubMed] [Google Scholar]
  5. Bird A. G., Britton S. A new approach to the study of human B lymphocyte function using an indirect plaque assay and a direct B cell activator. Immunol Rev. 1979;45:41–67. doi: 10.1111/j.1600-065x.1979.tb00272.x. [DOI] [PubMed] [Google Scholar]
  6. Coutinho A., Möller G. Editorial: Immune activation of B cells: evidence for 'one nonspecific triggering signal' not delivered by the Ig receptors. Scand J Immunol. 1974;3(2):133–146. [PubMed] [Google Scholar]
  7. Dutton R. W. Inhibitory and stimulatory effects of concanavalin A on the response of mouse spleen cell suspensions to antigen. I. Characterization of the inhibitory cell activity. J Exp Med. 1972 Dec 1;136(6):1445–1460. doi: 10.1084/jem.136.6.1445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fleisher T. A., Greene W. C., Blaese R. M., Waldmann T. A. Soluble suppressor supernatants elaborated by concanavalin A-activated human mononuclear cells. II. Characterization of a soluble suppressor of B cell immunoglobulin production. J Immunol. 1981 Mar;126(3):1192–1197. [PubMed] [Google Scholar]
  9. Gisler R. H., Fridman W. H. Suppression of in vitro antibody synthesis by immunoglobulin-binding factor. J Exp Med. 1975 Aug 1;142(2):507–517. doi: 10.1084/jem.142.2.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Greene W. C., Fleisher T. A., Waldmann T. A. Soluble suppressor supernatants elaborated by concanavalin A-activated human mononuclear cells. I. Characterization of a soluble suppressor T cell proliferation. J Immunol. 1981 Mar;126(3):1185–1191. [PubMed] [Google Scholar]
  11. Hofman F., Spina C. A., Rand N., Fahey J. L. Soluble suppressor factor produced by pokeweek mitogen-stimulated human peripheral blood leukocytes. Cell Immunol. 1980 Sep 15;55(1):20–31. doi: 10.1016/0008-8749(80)90133-1. [DOI] [PubMed] [Google Scholar]
  12. Honig S., Gorevic P., Weissmann G. C-reactive protein in systemic lupus erythematosus. Arthritis Rheum. 1977 Jun;20(5):1065–1070. doi: 10.1002/art.1780200505. [DOI] [PubMed] [Google Scholar]
  13. Jeffes E. W., Granger G. A. Relationship of cloning inhibition factor, "lymphotoxin" factor, and proliferation inhibition factor release in vitro by mitogen-activated human lymphoctes. J Immunol. 1975 Jan;114(1 Pt 1):64–69. [PubMed] [Google Scholar]
  14. Kadish A. S., Tansey F. A., Yu G. S., Doyle A. T., Bloom B. R. Interferon as a mediator of human lymphocyte suppression. J Exp Med. 1980 Mar 1;151(3):637–650. doi: 10.1084/jem.151.3.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kontiainen S., Feldmann M. Structural characteristics of antigen-specific suppressor factors: definition of 'constant' region and 'variable' region determinants. Thymus. 1979 Sep;1(1-2):59–79. [PubMed] [Google Scholar]
  16. Kontiainen S., Woody J. N., Rees A., Feldmann M. Induction of human antigen-specific suppressor factors in vitro. Clin Exp Immunol. 1981 Mar;43(3):517–525. [PMC free article] [PubMed] [Google Scholar]
  17. Menzoian J. O., Glasgow A. H., Nimberg R. D., Cooperband S. R., Schmid K., Saporoschetz I., Mannick J. A. Regulation of T lymphocyte function by immunoregulatory alphaglobulin (IRA). J Immunol. 1974 Jul;113(1):266–273. [PubMed] [Google Scholar]
  18. Mortensen R. F. Inhibition of the polyclonal antibody plaque-forming cell: response of human B lymphocytes by C-reactive protein (CRP) and CRP complexes. Cell Immunol. 1982 Jan 1;66(1):99–110. doi: 10.1016/0008-8749(82)90161-7. [DOI] [PubMed] [Google Scholar]
  19. Mortensen R. F., Osmand A. P., Gewurz H. Effects on C-reactive protein on the lymphoid system. I. Binding to thymus-dependent lymphocytes and alteration of their functions. J Exp Med. 1975 Apr 1;141(4):821–839. [PMC free article] [PubMed] [Google Scholar]
  20. Möller E., Ström H., Al-Balaghi S. Role of polyclonal activation in specific immune responses. Relevance for findings of antibody activity in various diseases. Scand J Immunol. 1980;12(3):177–182. doi: 10.1111/j.1365-3083.1980.tb00057.x. [DOI] [PubMed] [Google Scholar]
  21. Nordin A. A., Cosenza H., Sell S. Immunoglobulin classes of antibody-forming cells in mice. II. Class restriction of plaque-forming cells demonstrated by replica plating. J Immunol. 1970 Feb;104(2):495–501. [PubMed] [Google Scholar]
  22. Rich R. R., Pierce C. W. Biological expressions of lymphocyte activation. II. Generation of a population of thymus-derived suppressor lymphocytes. J Exp Med. 1973 Mar 1;137(3):649–659. doi: 10.1084/jem.137.3.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ringdén O., Rynnel-Dagö B., Waterfield E. M., Möller E., Möller G. Polyclonal antibody secretion in human lymphocytes induced by killed staphylococcal bacteria and by lipopolysaccharide. Scand J Immunol. 1977;6(11):1159–1169. doi: 10.1111/j.1365-3083.1977.tb00355.x. [DOI] [PubMed] [Google Scholar]
  24. Sheagren J. N., Block J. B., Trautman J. R., Wolff S. M. Immunologic reactivity in patients with leprosy. Ann Intern Med. 1969 Feb;70(2):295–302. doi: 10.7326/0003-4819-70-2-295. [DOI] [PubMed] [Google Scholar]
  25. Shou L., Schwartz S. A., Good R. A. Suppressor cell activity after concanavalin A treatment of lymphocytes from normal donors. J Exp Med. 1976 May 1;143(5):1100–1110. doi: 10.1084/jem.143.5.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tadakuma T., Pierce C. W. Mode of action of a soluble immune response suppressor (SIRS) produced by concanavalin a-activated spleen cells. J Immunol. 1978 Feb;120(2):481–486. [PubMed] [Google Scholar]
  27. Wangel A. G., Milton A., Egan J. B. Spontaneous plaque forming cells in the peripheral blood of patients with systemic lupus erythematosus. Clin Exp Immunol. 1982 Jul;49(1):41–49. [PMC free article] [PubMed] [Google Scholar]

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