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. 1991 Jul;85(1):70–74. doi: 10.1111/j.1365-2249.1991.tb05684.x

Failure to detect deposition of complement and immunoglobulin in allergen-induced late-phase skin reaction in atopic subjects.

A J Frew 1, A B Kay 1
PMCID: PMC1535708  PMID: 2070563

Abstract

There still remains some controversy regarding the possible role of immune complexes in the pathogenesis of the late-phase skin reaction (LPSR). To assess this, skin biopsies were obtained from LPSR induced in atopic human subjects 6, 24 and 48 h after allergen challenge. Cryostat sections were stained by direct immunofluorescence for the presence of fibrinogen, immunoglobulin classes IgM and IgG and for the complement components C1q and C3c. Complement components were observed in only two of the 29 biopsies studied. In both instances, only C3c was detected. One of these subjects also had unequivocal IgG staining at 6 h. IgM staining was detected in two out of 10 subjects at 6 h but no significant deposition of immunoglobulins could be found at 24 or 48 h. Fibrinogen deposition was observed in about half of the biopsies at each time-point. This study suggests that substantial complement and immunoglobulin deposition are not overt features of the allergen-induced LPSR, although the presence of small amounts of immune complexes, below the sensitivity of the method employed cannot be excluded. Fibrin deposition occurs in the LPSR but does not appear to be a prerequisite for LPSR development.

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Selected References

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  1. Archer C. B., Page C. P., Paul W., Morley J., MacDonald D. M. Inflammatory cell accumulation in response to intracutaneous Paf-acether: a mediator of acute and persistent inflammation? Br J Dermatol. 1985 Jul;113 (Suppl 28):133–135. doi: 10.1111/j.1365-2133.1985.tb15642.x. [DOI] [PubMed] [Google Scholar]
  2. Atkins P., Green G. R., Zweiman B. Histologic studies of human skin test responses to ragweed, compound 48-80, and histamine. J Allergy Clin Immunol. 1973 May;51(5):263–273. doi: 10.1016/0091-6749(73)90128-0. [DOI] [PubMed] [Google Scholar]
  3. Camp R. D., Coutts A. A., Greaves M. W., Kay A. B., Walport M. J. Responses of human skin to intradermal injection of leukotrienes C4, D4 and B4. Br J Pharmacol. 1983 Nov;80(3):497–502. doi: 10.1111/j.1476-5381.1983.tb10721.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cockcroft D. W., Ruffin R. E., Dolovich J., Hargreave F. E. Allergen-induced increase in non-allergic bronchial reactivity. Clin Allergy. 1977 Nov;7(6):503–513. doi: 10.1111/j.1365-2222.1977.tb01481.x. [DOI] [PubMed] [Google Scholar]
  5. Colvin R. B., Johnson R. A., Mihm M. C., Jr, Dvorak H. F. Role of the clotting system in cell-mediated hypersensitivity. I. Fibrin deposition in delayed skin reactions in man. J Exp Med. 1973 Sep 1;138(3):686–698. doi: 10.1084/jem.138.3.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dolovich J., Hargreave F. E., Chalmers R., Shier K. J., Gauldie J., Bienenstock J. Late cutaneous allergic responses in isolated IgE-dependent reactions. J Allergy Clin Immunol. 1973 Jul;52(1):38–46. doi: 10.1016/0091-6749(73)90119-x. [DOI] [PubMed] [Google Scholar]
  7. Dor P. J., Vervloet D., Sapene M., Andrac L., Bonerandi J. J., Charpin J. Induction of late cutaneous reaction by kallikrein injection: comparison with allergic-like late response to compound 48/80. J Allergy Clin Immunol. 1983 Apr;71(4):363–370. doi: 10.1016/0091-6749(83)90063-5. [DOI] [PubMed] [Google Scholar]
  8. Dorsch W., Ring J. Induction of late cutaneous reactions by skin-blister fluid from allergen-tested and normal skin. J Allergy Clin Immunol. 1981 Feb;67(2):117–123. doi: 10.1016/0091-6749(81)90006-3. [DOI] [PubMed] [Google Scholar]
  9. Faux J. A., Wide L., Hargreave F. E., Longbottom J. L., Pepys J. Immunological aspects of respiratory allergy in budgerigar (Melopsittacus undulatus) fanciers. Clin Allergy. 1971 Jun;1(2):149–158. doi: 10.1111/j.1365-2222.1971.tb03014.x. [DOI] [PubMed] [Google Scholar]
  10. Forman S. R., Fink J. N., Moore V. L., Wang J., Patterson R. Humoral and cellular immune responses in Aspergillus fumigatus pulmonary disease. J Allergy Clin Immunol. 1978 Sep;62(3):131–136. doi: 10.1016/0091-6749(78)90096-9. [DOI] [PubMed] [Google Scholar]
  11. Frew A. J., Kay A. B. The relationship between infiltrating CD4+ lymphocytes, activated eosinophils, and the magnitude of the allergen-induced late phase cutaneous reaction in man. J Immunol. 1988 Dec 15;141(12):4158–4164. [PubMed] [Google Scholar]
  12. James M. P., Kennedy A. R., Eady R. A. A microscopic study of inflammatory reactions in human skin induced by histamine and compound 48/80. J Invest Dermatol. 1982 May;78(5):406–413. doi: 10.1111/1523-1747.ep12507592. [DOI] [PubMed] [Google Scholar]
  13. Kay A. B., Ying S., Varney V., Gaga M., Durham S. R., Moqbel R., Wardlaw A. J., Hamid Q. Messenger RNA expression of the cytokine gene cluster, interleukin 3 (IL-3), IL-4, IL-5, and granulocyte/macrophage colony-stimulating factor, in allergen-induced late-phase cutaneous reactions in atopic subjects. J Exp Med. 1991 Mar 1;173(3):775–778. doi: 10.1084/jem.173.3.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Leiferman K. M., Fujisawa T., Gray B. H., Gleich G. J. Extracellular deposition of eosinophil and neutrophil granule proteins in the IgE-mediated cutaneous late phase reaction. Lab Invest. 1990 May;62(5):579–589. [PubMed] [Google Scholar]
  15. Medof M. E., Iida K., Mold C., Nussenzweig V. Unique role of the complement receptor CR1 in the degradation of C3b associated with immune complexes. J Exp Med. 1982 Dec 1;156(6):1739–1754. doi: 10.1084/jem.156.6.1739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pepys J. Immunopathology of allergic lung disease. Clin Allergy. 1973 Mar;3(1):1–22. doi: 10.1111/j.1365-2222.1973.tb01304.x. [DOI] [PubMed] [Google Scholar]
  17. Richerson H. B., Rajtora D. W., Penick G. D., Dick F. R., Yoo T. J., Kammermeyer J. K., Anuras J. S. Cutaneous and nasal allergic responses in ragweed hay fever: lack of clinical and histopathologic correlations with late phase reactions. J Allergy Clin Immunol. 1979 Jul;64(1):67–77. doi: 10.1016/0091-6749(79)90085-x. [DOI] [PubMed] [Google Scholar]
  18. Robertson D. G., Kerigan A. T., Hargreave F. E., Chalmers R., Dolovich J. Late asthmatic responses induced by ragweed pollen allergen. J Allergy Clin Immunol. 1974 Oct;54(4):244–254. doi: 10.1016/0091-6749(74)90067-0. [DOI] [PubMed] [Google Scholar]
  19. Senior R. M., Skogen W. F., Griffin G. L., Wilner G. D. Effects of fibrinogen derivatives upon the inflammatory response. Studies with human fibrinopeptide B. J Clin Invest. 1986 Mar;77(3):1014–1019. doi: 10.1172/JCI112353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Solley G. O., Gleich G. J., Jordon R. E., Schroeter A. L. The late phase of the immediate wheal and flare skin reaction. Its dependence upon IgE antibodies. J Clin Invest. 1976 Aug;58(2):408–420. doi: 10.1172/JCI108485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Taylor G., Shivalkar P. R. 'Arthus-type' reactivity in the nasal airways and skin in pollen sensitive subjects. Clin Allergy. 1971 Dec;1(4):407–414. doi: 10.1111/j.1365-2222.1971.tb00792.x. [DOI] [PubMed] [Google Scholar]
  22. Warren C. P., Tse K. S., Cherniack R. M. Mechanical properties of the lung in extrinsic allergic alveolitis. Thorax. 1978 Jun;33(3):315–321. doi: 10.1136/thx.33.3.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yokota A., Kikutani H., Tanaka T., Sato R., Barsumian E. L., Suemura M., Kishimoto T. Two species of human Fc epsilon receptor II (Fc epsilon RII/CD23): tissue-specific and IL-4-specific regulation of gene expression. Cell. 1988 Nov 18;55(4):611–618. doi: 10.1016/0092-8674(88)90219-x. [DOI] [PubMed] [Google Scholar]
  24. Zweiman B. Mediators of allergic inflammation in the skin. Clin Allergy. 1988 Sep;18(5):419–433. doi: 10.1111/j.1365-2222.1988.tb02892.x. [DOI] [PubMed] [Google Scholar]
  25. deShazo R. D., Levinson A. I., Dvorak H. F., Davis R. W. The late phase skin reaction: evidence for activation of the coagulation system in an IgE-dependent reaction in man. J Immunol. 1979 Feb;122(2):692–698. [PubMed] [Google Scholar]

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