Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1983 May;52(2):297–304.

Radioimmunoassay for antibodies to human skeletal muscle myosin in serum from patients with polymyositis.

K Wada, S Ueno, T Hazama, S Ogasahara, J Kang, M Takahashi, S Tarui
PMCID: PMC1535834  PMID: 6861376

Abstract

Antibodies to human skeletal muscle myosin were detected in 90% of sera from patients with polymyositis by radioimmunoassay using purified human myosin as antigen, and the mean titre was 4.24 X 10(-10)M. The incidence and the mean titre of anti-myosin antibodies were significantly higher than in patients without polymyositis. Anti-myosin antibody titres correlated with steroid therapy in polymyositis patients. Titres in untreated patients with polymyositis correlated with the severity of muscle weakness. Radioimmunoassay for anti-myosin antibodies should prove to be useful in the diagnosis of polymyositis and the evaluation of clinical state.

Full text

PDF
297

Images in this article

300Fig. 1
on p.300

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARWICK D. D., WALTON J. N. POLYMYOSITIS. Am J Med. 1963 Nov;35:646–660. doi: 10.1016/0002-9343(63)90136-0. [DOI] [PubMed] [Google Scholar]
  2. Behan W. M., Behan P. O. Complement abnormalities in polymyositis. J Neurol Sci. 1977 Nov;34(2):241–246. doi: 10.1016/0022-510x(77)90072-7. [DOI] [PubMed] [Google Scholar]
  3. CASPARY E. A., GUBBAY S. S., STERN G. M. CIRCULATING ANTIBODIES IN POLYMYOSITIS AND OTHER MUSCLE-WASTING DISORDERS. Lancet. 1964 Oct 31;2(7366):941–941. doi: 10.1016/s0140-6736(64)90868-2. [DOI] [PubMed] [Google Scholar]
  4. Currie S., Saunders M., Knowles M., Brown A. E. Immunological aspects of polymyositis. The in vitro activity of lymphocytes on incubation with muscle antigen and with muscle cultures. Q J Med. 1971 Jan;40(157):63–84. [PubMed] [Google Scholar]
  5. Dawkins R. L., Mastaglia F. L. Cell-mediated cytotoxicity to muscle in polymyositis. Effect of immunosuppression. N Engl J Med. 1973 Mar 1;288(9):434–438. doi: 10.1056/NEJM197303012880903. [DOI] [PubMed] [Google Scholar]
  6. Fessel W. J., Raas M. C. Autoimmunity in the pathogenesis of muscle disease. Neurology. 1968 Nov;18(11):1137–1139. doi: 10.1212/wnl.18.11.1137. [DOI] [PubMed] [Google Scholar]
  7. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kakulas B. A., Shute G. H., Leclere A. L. In vitro destruction of human foetal muscle cultures by peripheral blood lymphocytes from patients with polymyositis and lupus erythematosus. Proc Aust Assoc Neurol. 1971;8:85–92. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Libera L. D., Margreth A., Mussini I., Cerri C., Scarlato G. Myosin polymorphism in human skeletal muscles. Muscle Nerve. 1978 Jul-Aug;1(4):280–291. doi: 10.1002/mus.880010404. [DOI] [PubMed] [Google Scholar]
  12. Lisak R. P., Zweiman B. Serum immunogloblin levels in myasthenia gravis, polymyositis, and dermatomyositis. J Neurol Neurosurg Psychiatry. 1976 Jan;39(1):34–37. doi: 10.1136/jnnp.39.1.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McDonald B. L., Dawkins R. L., Robinson J. Myosin autoantibodies reacting with selective muscle fiber types. Muscle Nerve. 1979 Jan-Feb;2(1):37–43. doi: 10.1002/mus.880020106. [DOI] [PubMed] [Google Scholar]
  14. Nishikai M., Homma M. Circulating autoantibody against human myoglobin in polymyositis. JAMA. 1977 Apr 25;237(17):1842–1844. [PubMed] [Google Scholar]
  15. Nishikai M., Reichlin M. Heterogeneity of precipitating antibodies in polymyositis and dermatomyositis. Characterization of the Jo-1 antibody system. Arthritis Rheum. 1980 Aug;23(8):881–888. doi: 10.1002/art.1780230802. [DOI] [PubMed] [Google Scholar]
  16. Nishikai M., Reichlin M. Purification and characterization of a nuclear non-histone basic protein (Mi-1) which reacts with anti-immunoglobulin sera and the sera of patients with dermatomyositis. Mol Immunol. 1980 Sep;17(9):1129–1141. doi: 10.1016/0161-5890(80)90109-1. [DOI] [PubMed] [Google Scholar]
  17. Offer G. The antigenicity of myosin and C-protein. Proc R Soc Lond B Biol Sci. 1976 Mar 16;192(1109):439–449. doi: 10.1098/rspb.1976.0022. [DOI] [PubMed] [Google Scholar]
  18. Reichlin M., Mattioli M. Description of a serological reaction characteristic of polymyositis. Clin Immunol Immunopathol. 1976 Jan;5(1):12–20. doi: 10.1016/0090-1229(76)90145-8. [DOI] [PubMed] [Google Scholar]
  19. Richards E. G., Chung C. S., Menzel D. B., Olcott H. S. Chromatography of myosin on diethylaminoethyl-Sephadex A-50. Biochemistry. 1967 Feb;6(2):528–540. doi: 10.1021/bi00854a022. [DOI] [PubMed] [Google Scholar]
  20. Samaha F. J., Gergely J. Biochemistry of normal and myotonic dystrophic human myosin. Arch Neurol. 1969 Aug;21(2):200–207. doi: 10.1001/archneur.1969.00480140100010. [DOI] [PubMed] [Google Scholar]
  21. Samaha F. J., Thies W. H. Myosin light chains in Duchenne dystrophy and paraplegic muscle. Neurology. 1979 Jan;29(1):122–125. doi: 10.1212/wnl.29.1.122. [DOI] [PubMed] [Google Scholar]
  22. Sreter F. A., Aström K. E., Romanul F. C., Young R. R., Jones H. R., Jr Characteristics of myosin in nemaline myopathy. J Neurol Sci. 1976 Jan;27(1):99–116. doi: 10.1016/0022-510x(76)90238-0. [DOI] [PubMed] [Google Scholar]
  23. Starr R., Offer G. Polypeptide chains of intermediate molecular weight in myosin preparations. FEBS Lett. 1971 Jun 2;15(1):40–44. doi: 10.1016/0014-5793(71)80075-3. [DOI] [PubMed] [Google Scholar]
  24. Stern G. M., Rose A. L., Jacobs K. Circulating antibodies in polymyositis. J Neurol Sci. 1967 Jul-Aug;5(1):181–183. doi: 10.1016/0022-510x(67)90015-9. [DOI] [PubMed] [Google Scholar]
  25. Wolfe J. F., Adelstein E., Sharp G. C. Antinuclear antibody with distinct specificity for polymyositis. J Clin Invest. 1977 Jan;59(1):176–178. doi: 10.1172/JCI108616. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES