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. 1983 Nov;54(2):567–572.

Stimulation of lectin-dependent cell-mediated cytotoxicity against adherent HEp-2 cells by carrageenan.

A Perl, R Gonzalez-Cabello, P Gergely
PMCID: PMC1535892  PMID: 6652971

Abstract

The effect of carrageenan (CGN) was studied on lectin-dependent cell-mediated cytotoxicity (LDCC) against adherent HEp-2 human epipharynx carcinoma target cells. Cytotoxicity was measured by detachment from the monolayer of 3H-TdR pre-labelled HEp-2 cells. LDCC was evaluated in a 24 h assay at 50:1 effector-target cell ratio in the presence of 25 micrograms/ml concanavalin A (Con A). Under these conditions but without Con A considerable cytotoxicity was not elicited by peripheral blood mononuclear cells (PBMC). Addition of CGN to the system significantly increased LDCC to HEp-2 targets. Monocyte depletion of effector cells had no major influence to the effect of CGN on LDCC activity. In parallel studies CGN also enhanced the Con A-induced proliferation of PBMC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonavida B., Bradley T. P. Studies on the induction and expression of T cell-mediated immunity. V. Lectin-induced nonspecific cell-mediated cytotoxicity by alloimmune lymphocytes. Transplantation. 1976 Feb;21(2):94–102. doi: 10.1097/00007890-197602000-00002. [DOI] [PubMed] [Google Scholar]
  2. Catanzaro P. J., Schwartz H. J., Graham R. C., Jr Spectrum and possible mechanism of carrageenan cytotoxicity. Am J Pathol. 1971 Aug;64(2):387–404. [PMC free article] [PubMed] [Google Scholar]
  3. Green W. R., Ballas Z. K., Henney C. S. Studies on the mechanism of lymphocyte-mediated cytolysis. XI. The role of lectin in lectin-dependent cell-mediated cytotoxicity. J Immunol. 1978 Oct;121(4):1566–1572. [PubMed] [Google Scholar]
  4. HOLM G., PERLMANN P., WERNER B. PHYTOHAEMAGGLUTININ-INDUCED CYTOTOXIC ACTION OF NORMAL LYMPHOID CELLS ON CELLS IN TISSUE CULTURE. Nature. 1964 Aug 22;203:841–843. doi: 10.1038/203841a0. [DOI] [PubMed] [Google Scholar]
  5. Horwitz D. A., Allison A. C., Ward P., Kight N. Identification of human mononuclear leucocyte populations by esterase staining. Clin Exp Immunol. 1977 Nov;30(2):289–298. [PMC free article] [PubMed] [Google Scholar]
  6. Huges-Law G., de Gast G. C., The T. H. PHA-induced cytotoxicity of human lymphocytes against adherent hela cells. J Immunol Methods. 1978;19(1):29–39. doi: 10.1016/0022-1759(78)90004-2. [DOI] [PubMed] [Google Scholar]
  7. Ishizaka S., Hasuma T., Otani S., Morisawa S. Lymphocyte activation by purified carrageenan. J Immunol. 1980 Nov;125(5):2232–2235. [PubMed] [Google Scholar]
  8. Jondal M., Targan S. Spontaneous and lectin-dependent cellular cytotoxicity by lymphocyte subpopulations against cell lines susceptible or resistant to spontaneous cytotoxicity. Clin Exp Immunol. 1978 Jul;33(1):121–127. [PMC free article] [PubMed] [Google Scholar]
  9. Kataoka T., Oh-hashi F., Sakurai Y., Gomi K. In vivo potentiation of concanavalin A-bound L1210 vaccine by antimacrophage agents. Cancer Res. 1980 Oct;40(10):3832–3838. [PubMed] [Google Scholar]
  10. MacDermott R. P., Kienker L. J., Bertovich M. J., Muchmore A. V. Inhibition of spontaneous but not antibody-dependent cell-mediated cytotoxicity by simple sugars: evidence that endogenous lectins may mediate spontaneous cell-mediated cytotoxicity. Immunology. 1981 Sep;44(1):143–152. [PMC free article] [PubMed] [Google Scholar]
  11. Parker B. M., Laux D. C. Selective induction and inhibition of direct and lectin-dependent cell-mediated cytotoxic reactivity. Cell Immunol. 1981 Jul 1;61(2):253–264. doi: 10.1016/0008-8749(81)90373-7. [DOI] [PubMed] [Google Scholar]
  12. Perl A., Pócsik E., Gonzalez-Cabello R., Láng I., Gergely P. Human lectin-dependent T cell-mediated cytotoxicity against Hep-2 cells. Acta Physiol Hung. 1983;61(4):225–231. [PubMed] [Google Scholar]
  13. Schubert M. S., Lucas D. O. Antiautologous lectin-dependent cell-mediated cytotoxicity: evidence for prekiller cells as effector cells. Cell Immunol. 1981 May 1;60(1):11–23. doi: 10.1016/0008-8749(81)90243-4. [DOI] [PubMed] [Google Scholar]
  14. Sugawara I., Ishizaka S. Role of various carrageenans in autologous and allogenic mixed lymphocyte reaction. Cell Immunol. 1982 Apr;68(2):402–409. doi: 10.1016/0008-8749(82)90124-1. [DOI] [PubMed] [Google Scholar]
  15. Vose B. M., Harding M., White W., Moore M., Gallagher J. Effect of simple sugars on natural killing: evidence against the involvement of a lectin like mechanism in target recognition. Clin Exp Immunol. 1983 Mar;51(3):517–524. [PMC free article] [PubMed] [Google Scholar]
  16. Weston P. A., Levy N. L., Koren H. S. Spontaneous cytoxicity against virus-infected cells: cellular immunoadsorption on infected cell monolayers. J Immunol. 1980 Sep;125(3):1387–1394. [PubMed] [Google Scholar]
  17. Yue C. L., Tanimoto K., Horiuchi Y. Characterization and possible mechanisms of mitogen-induced cell-mediated cytotoxicity. Scand J Immunol. 1981 Oct;14(4):397–408. doi: 10.1111/j.1365-3083.1981.tb00580.x. [DOI] [PubMed] [Google Scholar]

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