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. 1983 Nov;54(2):327–336.

Monoclonal antibodies to human leucocyte antigens in polymyositis and muscular dystrophy.

D Rowe, D A Isenberg, P C Beverley
PMCID: PMC1535911  PMID: 6228350

Abstract

Biopsy specimens from patients with treated or untreated polymyositis and muscular dystrophy controls were examined by indirect immunoperoxidase staining with a panel of monoclonal antibodies to human leucocyte antigens. In untreated polymyositis, helper/inducer T cells were the predominant T cell subset. In treated cases few T cells were seen. Overall, few T cells were seen in dystrophic cases, most infiltrating cells being dendritic and lacking T cell antigens. Staining of sarcolemma with anti-HLA class 1 antibody is weak or negative except in areas adjacent to infiltrating leucocytes or where muscle fibre damage is apparent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Bassat M., Machtey I. Picornavirus-like structures in acute dermatomyositis. Am J Clin Pathol. 1972 Sep;58(3):245–249. doi: 10.1093/ajcp/58.3.245. [DOI] [PubMed] [Google Scholar]
  2. Beverley P. C., Callard R. E. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981 Apr;11(4):329–334. doi: 10.1002/eji.1830110412. [DOI] [PubMed] [Google Scholar]
  3. Bohan A., Peter J. B. Polymyositis and dermatomyositis (second of two parts). N Engl J Med. 1975 Feb 20;292(8):403–407. doi: 10.1056/NEJM197502202920807. [DOI] [PubMed] [Google Scholar]
  4. Brodsky F. M., Parham P., Barnstable C. J., Crumpton M. J., Bodmer W. F. Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979;47:3–61. doi: 10.1111/j.1600-065x.1979.tb00288.x. [DOI] [PubMed] [Google Scholar]
  5. Bunch T. W., Worthington J. W., Combs J. J., Ilstrup D. M., Engel A. G. Azathioprine with prednisone for polymyositis. A controlled, clinical trial. Ann Intern Med. 1980 Mar;92(3):365–369. doi: 10.7326/0003-4819-92-3-365. [DOI] [PubMed] [Google Scholar]
  6. Chou S. M., Gutmann L. Picornavirus-like crystals in subacute polymyositis. Neurology. 1970 Mar;20(3):205–213. doi: 10.1212/wnl.20.3.205. [DOI] [PubMed] [Google Scholar]
  7. DeVere R., Bradley W. G. Polymyositis: its presentation, morbidity and mortality. Brain. 1975 Dec;98(4):637–666. doi: 10.1093/brain/98.4.637. [DOI] [PubMed] [Google Scholar]
  8. Duke O., Panayi G. S., Janossy G., Poulter L. W. An immunohistological analysis of lymphocyte subpopulations and their microenvironment in the synovial membranes of patients with rheumatoid arthritis using monoclonal antibodies. Clin Exp Immunol. 1982 Jul;49(1):22–30. [PMC free article] [PubMed] [Google Scholar]
  9. Fox R. I., Carstens S. A., Fong S., Robinson C. A., Howell F., Vaughan J. H. Use of monoclonal antibodies to analyze peripheral blood and salivary gland lymphocyte subsets in Sjögren's syndrome. Arthritis Rheum. 1982 Apr;25(4):419–426. doi: 10.1002/art.1780250410. [DOI] [PubMed] [Google Scholar]
  10. Janossy G., Panayi G., Duke O., Bofill M., Poulter L. W., Goldstein G. Rheumatoid arthritis: a disease of T-lymphocyte/macrophage immunoregulation. Lancet. 1981 Oct 17;2(8251):839–842. doi: 10.1016/s0140-6736(81)91107-7. [DOI] [PubMed] [Google Scholar]
  11. Kessler H. A., Trenholme G. M., Harris A. A., Levin S. Acute myopathy associated with influenza A/Texas/1/77 infection. Isolation of virus from a muscle biopsy specimen. JAMA. 1980 Feb 1;243(5):461–462. [PubMed] [Google Scholar]
  12. Ledbetter J. A., Evans R. L., Lipinski M., Cunningham-Rundles C., Good R. A., Herzenberg L. A. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981 Feb 1;153(2):310–323. doi: 10.1084/jem.153.2.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mastaglia F. L., Walton J. N. Coxsackie virus-like particles in skeletal muscle from a case of polymyositis. J Neurol Sci. 1970 Dec;11(6):593–599. doi: 10.1016/0022-510x(70)90109-7. [DOI] [PubMed] [Google Scholar]
  14. McMichael A. HLA restriction of human cytotoxic T lymphocytes specific for influenza virus. Poor recognition of virus associated with HLA A2. J Exp Med. 1978 Dec 1;148(6):1458–1467. doi: 10.1084/jem.148.6.1458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Medsger T. A., Jr, Robinson H., Masi A. T. Factors affecting survivorship in polymyositis. A life-table study of 124 patients. Arthritis Rheum. 1971 Mar-Apr;14(2):249–258. doi: 10.1002/art.1780140210. [DOI] [PubMed] [Google Scholar]
  16. Morimoto C., Reinherz E. L., Nadler L. M., Distaso J. A., Steinberg A. D., Schlossman S. F. Comparison in T- and B-cell markers in patients with Sjögren's syndrome and systemic lupus erythematosus. Clin Immunol Immunopathol. 1982 Feb;22(2):270–278. doi: 10.1016/0090-1229(82)90043-5. [DOI] [PubMed] [Google Scholar]
  17. Riddoch D., Morgan-Hughes J. A. Prognosis in adult polymyositis. J Neurol Sci. 1975 Sep;26(1):71–80. doi: 10.1016/0022-510x(75)90115-x. [DOI] [PubMed] [Google Scholar]
  18. Rowe D. J., Isenberg D. A., McDougall J., Beverley P. C. Characterization of polymyositis infiltrates using monoclonal antibodies to human leucocyte antigens. Clin Exp Immunol. 1981 Aug;45(2):290–298. [PMC free article] [PubMed] [Google Scholar]
  19. Sato T., Walker D. L., Peters H. A., Resse H. H., Chou S. M. Chronic polymyositis and myxovirus-like inclusions. Electron microscopic and viral studies. Arch Neurol. 1971 May;24(5):409–418. doi: 10.1001/archneur.1971.00480350043004. [DOI] [PubMed] [Google Scholar]
  20. Wallach D., Fellous M., Revel M. Preferential effect of gamma interferon on the synthesis of HLA antigens and their mRNAs in human cells. Nature. 1982 Oct 28;299(5886):833–836. doi: 10.1038/299833a0. [DOI] [PubMed] [Google Scholar]

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