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. 1984 Mar;55(3):502–508.

Cross-reactivity of anti-DNA antibodies with proteoglycans.

P Faaber, P J Capel, G P Rijke, G Vierwinden, L B van de Putte, R A Koene
PMCID: PMC1535916  PMID: 6608423

Abstract

Ten sera of patients with systemic lupus erythematosus (SLE) were tested in an enzyme linked immunosorbent assay for their ability to react with glycosaminoglycans, constituents of proteoglycans, in relation to their anti-DNA reactivity. The SLE sera reacted with hyaluronic acid and chondroitin sulphate and this reactivity correlated with the anti-DNA activity of these sera. By contrast, sera obtained from patients with other autoimmune diseases or normal sera lacked any of these reactivities. Anti-DNA antibodies purified by affinity chromatography with either oligo dT cellulose or Cibracon blue F3Ga Sepharose reacted with DNA as well as with hyaluronic acid. The cross-reactivity of anti-DNA antibodies could be confirmed by the reaction of a mouse monoclonal anti-DNA antibody with DNA, hyaluronic acid, and chondroitin sulphate. This pattern of cross-reactivities of anti-DNA antibodies suggests that several compounds can function as antigenic targets for these antibodies provided that their structures contain repeating negatively charged groups.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aarden L. A., Lakmaker F., Feltkamp T. E. Immunology of DNA. I. The influence of reaction conditions on the Farr assay as used for the detection of anti-ds DNA. J Immunol Methods. 1976;10(1):27–37. doi: 10.1016/0022-1759(76)90004-1. [DOI] [PubMed] [Google Scholar]
  2. Aarden L. A., de Groot E. R., Feltkamp T. E. Immunology of DNA. III. Crithidia luciliae, a simple substrate for the determination of anti-dsDNA with the immunofluorescence technique. Ann N Y Acad Sci. 1975 Jun 30;254:505–515. doi: 10.1111/j.1749-6632.1975.tb29197.x. [DOI] [PubMed] [Google Scholar]
  3. Arana R., Seligmann M. Antibodies to native and denatured deoxyribonucleic acid in systemic lupus erythematosus. J Clin Invest. 1967 Nov;46(11):1867–1882. doi: 10.1172/JCI105677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DEICHER H. R., HOLMAN H. R., KUNKEL H. G. The precipitin reaction between DNA and a serum factor in systemic lupus erythematosus. J Exp Med. 1959 Jan 1;109(1):97–114. doi: 10.1084/jem.109.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eaton R. B., Schnneider G., Schur P. H. Enzyme immunoassay for antibodies to native DNA. Specificity and quality of antibodies. Arthritis Rheum. 1983 Jan;26(1):52–62. doi: 10.1002/art.1780260109. [DOI] [PubMed] [Google Scholar]
  6. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  7. Kanwar Y. S., Farquhar M. G. Anionic sites in the glomerular basement membrane. In vivo and in vitro localization to the laminae rarae by cationic probes. J Cell Biol. 1979 Apr;81(1):137–153. doi: 10.1083/jcb.81.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koffler D., Carr R., Agnello V., Thoburn R., Kunkel H. G. Antibodies to polynucleotides in human sera: antigenic specificity and relation to disease. J Exp Med. 1971 Jul 1;134(1):294–312. doi: 10.1084/jem.134.1.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Koike T., Tomioka H., Kumagai A. Antibodies cross-reactive with DNA and cardiolipin in patients with systemic lupus erythematosus. Clin Exp Immunol. 1982 Nov;50(2):298–302. [PMC free article] [PubMed] [Google Scholar]
  10. Lafer E. M., Rauch J., Andrzejewski C., Jr, Mudd D., Furie B., Furie B., Schwartz R. S., Stollar B. D. Polyspecific monoclonal lupus autoantibodies reactive with both polynucleotides and phospholipids. J Exp Med. 1981 Apr 1;153(4):897–909. doi: 10.1084/jem.153.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pollard K. M., Webb J. Partial purification of anti-DNA antibodies from systemic lupus erythematosus serum by dye-ligand chromatography. J Immunol Methods. 1982 Oct 15;54(1):81–94. doi: 10.1016/0022-1759(82)90116-8. [DOI] [PubMed] [Google Scholar]
  12. STOLLAR D., LEVINE L., LEHRER H. I., VAN VUNAKIS H. The antigenic determinants of denatured DNA reactive with lupus erythematosus serum. Proc Natl Acad Sci U S A. 1962 May 15;48:874–880. doi: 10.1073/pnas.48.5.874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shoenfeld Y., Rauch J., Massicotte H., Datta S. K., André-Schwartz J., Stollar B. D., Schwartz R. S. Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas. N Engl J Med. 1983 Feb 24;308(8):414–420. doi: 10.1056/NEJM198302243080802. [DOI] [PubMed] [Google Scholar]
  14. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]

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