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. 1984 Mar;55(3):553–560.

Diversity of autoantibodies in primary biliary cirrhosis and chronic active hepatitis.

R M Bernstein, J M Neuberger, C C Bunn, M E Callender, G R Hughes, R Williams
PMCID: PMC1535919  PMID: 6368058

Abstract

The presence of autoantibodies in the serum of 110 patients with primary biliary cirrhosis (PBC), 50 with HBsAg negative chronic active hepatitis (HBsAg- CAH) and 30 with HBsAg positive chronic active hepatitis (HBsAg+ CAH) was assessed using two methods: indirect immunofluorescence on cells grown in tissue culture (HEp-2 cell line) or standard mouse tissue sections, and counter immunoelectrophoresis (CIE) with soluble tissue extracts. Anti-nuclear antibodies (ANA) were found in 38% of sera from patients with PBC using HEp-2 cells compared with 10% using mouse tissue. A variety of staining patterns were detected including a pattern of multiple nuclear dots. In contrast, ANA was detected in 70% of sera from patients with HBsAg- CAH and 27% with HBsAg+ CAH. Using CIE four distinct antibody antigen systems were detected: Ro (SS-A), La (SS-B) and two new systems, designated XH and XR, reacting with extracts of human spleen and rabbit thymus, respectively. Correlation of the presence of antibody with clinical conditions confirmed the close association between anti-centromere antibody and sclerodactyly in patients with PBC and indicated an association between 'multiple nuclear dot' staining and the sicca syndrome in PBC. No association was found between the presence of either Ro or La antibody and the sicca syndrome in patients with PBC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agutter P. S., Richardson J. C. Nuclear non-chromatin proteinaceous structures: their role in the organization and function of the interphase nucleus. J Cell Sci. 1980 Aug;44:395–435. doi: 10.1242/jcs.44.1.395. [DOI] [PubMed] [Google Scholar]
  2. Alspaugh M., Maddison P. Resolution of the identity of certain antigen-antibody systems in systemic lupus erythematosus and Sjögren's syndrome: an interlaboratory collaboration. Arthritis Rheum. 1979 Jul;22(7):796–798. doi: 10.1002/art.1780220719. [DOI] [PubMed] [Google Scholar]
  3. Bernstein R. M., Bunn C. C., Hughes G. R. Identification of antibodies to acidic antigens by counterimmunoelectrophoresis. Ann Rheum Dis. 1982 Oct;41(5):554–555. doi: 10.1136/ard.41.5.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bernstein R. M., Callender M. E., Neuberger J. M., Hughes G. R., Williams R. Anticentromere antibody in primary biliary cirrhosis. Ann Rheum Dis. 1982 Dec;41(6):612–614. doi: 10.1136/ard.41.6.612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bernstein R. M., Steigerwald J. C., Tan E. M. Association of antinuclear and antinucleolar antibodies in progressive systemic sclerosis. Clin Exp Immunol. 1982 Apr;48(1):43–51. [PMC free article] [PubMed] [Google Scholar]
  6. Burnham T. K., Bank P. W. Antinuclear antibodies. I. Patterns of nuclear immunofluorescence. J Invest Dermatol. 1974 May;62(5):526–534. doi: 10.1111/1523-1747.ep12681110. [DOI] [PubMed] [Google Scholar]
  7. De Groote J., Desmet V. J., Gedigk P., Korb G., Popper H., Poulsen H., Scheuer P. J., Schmid M., Thaler H., Uehlinger E. A classification of chronic hepatitis. Lancet. 1968 Sep 14;2(7568):626–628. doi: 10.1016/s0140-6736(68)90710-1. [DOI] [PubMed] [Google Scholar]
  8. Doniach D., Roitt I. M., Walker J. G., Sherlock S. Tissue antibodies in primary biliary cirrhosis, active chronic (lupoid) hepatitis, cryptogenic cirrhosis and other liver diseases and their clinical implications. Clin Exp Immunol. 1966 Jul;1(3):237–262. [PMC free article] [PubMed] [Google Scholar]
  9. Hamlyn A. N., Adams D., Sherlock S. Primary or secondary sicca complex? Investigation in primary biliary cirrhosis by histocompatibility testing. Br Med J. 1980 Aug 9;281(6237):425–426. doi: 10.1136/bmj.281.6237.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miyachi K., Gupta R. C., Dickson E. R., Tan E. M. Preciitating antibodies to mitochondrial antigens in patients with primary biliary cirrhosis. Clin Exp Immunol. 1980 Mar;39(3):599–606. [PMC free article] [PubMed] [Google Scholar]
  11. Moroi Y., Hartman A. L., Nakane P. K., Tan E. M. Distribution of kinetochore (centromere) antigen in mammalian cell nuclei. J Cell Biol. 1981 Jul;90(1):254–259. doi: 10.1083/jcb.90.1.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moutsopoulos H. M., Mann D. L., Johnson A. H., Chused T. M. Genetic differences between primary and secondary sicca syndrome. N Engl J Med. 1979 Oct 4;301(14):761–763. doi: 10.1056/NEJM197910043011405. [DOI] [PubMed] [Google Scholar]
  13. Schaffner F., Popper H. Clinical-pathologic relations in primary biliary cirrhosis. Prog Liver Dis. 1982;7:529–554. [PubMed] [Google Scholar]
  14. Tan E. M. Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol. 1982;33:167–240. doi: 10.1016/s0065-2776(08)60836-6. [DOI] [PubMed] [Google Scholar]

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