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. 1984 Mar;55(3):643–650.

Enhanced interleukin activity following asbestos inhalation.

D P Hartmann, M M Georgian, Y Oghiso, E Kagan
PMCID: PMC1535921  PMID: 6608427

Abstract

Asbestos inhalation can cause pulmonary fibrosis and is associated with a variety of immunological abnormalities. The purpose of this study was to evaluate the effects of asbestos inhalation on interleukin-1 (IL-1) and interleukin-2 (IL-2) production in a rodent model. Two groups of rats were exposed, by intermittent inhalation, to either amphibole (crocidolite) or serpentine (chrysotile) asbestos. A third (control) group of rats was sham exposed to clean air. Animals from the three exposure groups were thereafter immunized (or not immunized) with fetal calf serum antigens. In order to assay interleukin activity, supernatants were generated from cultures containing alveolar macrophages and autologous splenic lymphocytes, and from cultures containing alveolar macrophages alone. Using assay systems designed to detect IL-1 and IL-2 functional activity, the supernatants were evaluated for their capacity to stimulate lymphoproliferation and fibroblast DNA synthesis. Macrophage-lymphocyte co-culture supernatants, when obtained from immunized, asbestos exposed rats, contained greater IL-1 and IL-2 activity than identical supernatants from immunized, sham exposed animals. These between group differences were not, however, observed in supernatants from unimmunized rats, or when supernatants were generated in the absence of immune lymphocytes. These observations suggest that asbestos exposure is associated with enhanced activation of lymphocytes by antigens. The possible relevance of these findings to asbestos related fibrogenesis and immunological stimulation is discussed.

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Selected References

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  1. Bateman E. D., Emerson R. J., Cole P. J. A study of macrophage-mediated initiation of fibrosis by asbestos and silica using a diffusion chamber technique. Br J Exp Pathol. 1982 Aug;63(4):414–425. [PMC free article] [PubMed] [Google Scholar]
  2. Becklake M. R. Asbestos-related diseases of the lungs and pleura: current clinical issues. Am Rev Respir Dis. 1982 Aug;126(2):187–194. doi: 10.1164/arrd.1982.126.2.187. [DOI] [PubMed] [Google Scholar]
  3. Bitterman P. B., Rennard S. I., Hunninghake G. W., Crystal R. G. Human alveolar macrophage growth factor for fibroblasts. Regulation and partial characterization. J Clin Invest. 1982 Oct;70(4):806–822. doi: 10.1172/JCI110677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davies P., Allison A. C., Ackerman J., Butterfield A., Williams S. Asbestos induces selective release of lysosomal enzymes from mononuclear phagocytes. Nature. 1974 Oct 4;251(5474):423–425. doi: 10.1038/251423a0. [DOI] [PubMed] [Google Scholar]
  5. Farr A. G., Kiely J. M., Unanue E. R. Macrophage-T cell interactions involving Listeria monocytogenes--role of the H-2 gene complex. J Immunol. 1979 Jun;122(6):2395–2404. [PubMed] [Google Scholar]
  6. Farrar J. J., Benjamin W. R., Hilfiker M. L., Howard M., Farrar W. L., Fuller-Farrar J. The biochemistry, biology, and role of interleukin 2 in the induction of cytotoxic T cell and antibody-forming B cell responses. Immunol Rev. 1982;63:129–166. doi: 10.1111/j.1600-065x.1982.tb00414.x. [DOI] [PubMed] [Google Scholar]
  7. Gaumer H. R., Doll N. J., Kaimal J., Schuyler M., Salvaggio J. E. Diminished suppressor cell function in patients with asbestosis. Clin Exp Immunol. 1981 Apr;44(1):108–116. [PMC free article] [PubMed] [Google Scholar]
  8. Gery I., Davies P., Derr J., Krett N., Barranger J. A. Relationship between production and release of lymphocyte-activating factor (interleukin 1) by murine macrophages. 1. Effects of various agents. Cell Immunol. 1981 Nov 1;64(2):293–303. doi: 10.1016/0008-8749(81)90481-0. [DOI] [PubMed] [Google Scholar]
  9. Heppleston A. G., Styles J. A. Activity of a macrophage factor in collagen formation by silica. Nature. 1967 Apr 29;214(5087):521–522. doi: 10.1038/214521a0. [DOI] [PubMed] [Google Scholar]
  10. Kagan E., Jacobson R. J. Lymphoid and plasma cell malignancies: asbestos-related disorders of long latency. Am J Clin Pathol. 1983 Jul;80(1):14–20. doi: 10.1093/ajcp/80.1.14. [DOI] [PubMed] [Google Scholar]
  11. Kagan E., Oghiso Y., Hartmann D. P. Enhanced release of a chemoattractant for alveolar macrophages after asbestos inhalation. Am Rev Respir Dis. 1983 Oct;128(4):680–687. doi: 10.1164/arrd.1983.128.4.680. [DOI] [PubMed] [Google Scholar]
  12. Kagan E., Solomon A., Cochrane J. C., Kuba P., Rocks P. H., Webster I. Immunological studies of patients with asbestosis. II, Studies of circulating lymphoid cell numbers and humoral immunity. Clin Exp Immunol. 1977 May;28(2):268–275. [PMC free article] [PubMed] [Google Scholar]
  13. Kagan E. The alveolar macrophage: immune derangement and asbestos-related malignancy. Semin Oncol. 1981 Sep;8(3):258–267. [PubMed] [Google Scholar]
  14. Lachman L. B. Human interleukin 1: purification and properties. Fed Proc. 1983 Jun;42(9):2639–2645. [PubMed] [Google Scholar]
  15. Lange A., Smolik R., Zatoński W., Szymańska J. Autoantibodies and serum immunoglobulin levels in asbestos workers. Int Arch Arbeitsmed. 1974 Jan 31;32(4):313–325. doi: 10.1007/BF02178970. [DOI] [PubMed] [Google Scholar]
  16. Miller K., Kagan E. Immune adherence reactivity of rat alveolar macrophages following inhalation of crocidolite asbestos. Clin Exp Immunol. 1977 Jul;29(1):152–158. [PMC free article] [PubMed] [Google Scholar]
  17. Miller K., Kagan E. Manifestations of cellular immunity in the rat after prolonged asbestos inhalation. II. Alveolar macrophage-induced splenic lymphocyte proliferation. Environ Res. 1981 Oct;26(1):182–194. doi: 10.1016/0013-9351(81)90197-3. [DOI] [PubMed] [Google Scholar]
  18. Miller K., Weintraub Z., Kagan E. Manifestations of cellular immunity in the rat after prolonged asbestos inhalation. I. Physical interactions between alveolar macrophages and splenic lymphocytes. J Immunol. 1979 Sep;123(3):1029–1038. [PubMed] [Google Scholar]
  19. Miller L. G., Sparrow D., Ginns L. C. Asbestos exposure correlates with alterations in circulating T cell subsets. Clin Exp Immunol. 1983 Jan;51(1):110–116. [PMC free article] [PubMed] [Google Scholar]
  20. Oppenheim J. J., Stadler B. M., Siraganian R. P., Mage M., Mathieson B. Lymphokines: their role in lymphocyte responses. Properties of interleukin 1. Fed Proc. 1982 Feb;41(2):257–262. [PubMed] [Google Scholar]
  21. Pernis B., Vigliani E. C. The role of macrophages and immunocytes in the pathogenesis of pulmonary diseases due to mineral dusts. Am J Ind Med. 1982;3(2):133–137. doi: 10.1002/ajim.4700030203. [DOI] [PubMed] [Google Scholar]
  22. Pinkerton K. E., Brody A. R., McLaurin D. A., Adkins B., Jr, O'Connor R. W., Pratt P. C., Crapo J. D. Characterization of three types of chrysotile asbestos after aerosolization. Environ Res. 1983 Jun;31(1):32–53. doi: 10.1016/0013-9351(83)90060-9. [DOI] [PubMed] [Google Scholar]
  23. Rendall R. E. Physical and chemical characteristics of UICC reference samples. IARC Sci Publ. 1980;(30):87–96. [PubMed] [Google Scholar]
  24. Ross R., Dworsky R., Nichols P., Paganini-Hill A., Wright W., Koss M., Lukes R., Henderson B. Asbestos exposure and lymphomas of the gastrointestinal tract and oral cavity. Lancet. 1982 Nov 20;2(8308):1118–1120. doi: 10.1016/s0140-6736(82)92783-0. [DOI] [PubMed] [Google Scholar]
  25. Schmidt J. A., Mizel S. B., Cohen D., Green I. Interleukin 1, a potential regulator of fibroblast proliferation. J Immunol. 1982 May;128(5):2177–2182. [PubMed] [Google Scholar]
  26. Turner-Warwick M. Immunology and asbestosis. Proc R Soc Med. 1973 Sep;66(9):927–930. doi: 10.1177/003591577306600949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Weinberg D. S., Unanue E. R. Antigen-presenting function of alveolar macrophages: uptake and presentation of Listeria monocytogenes. J Immunol. 1981 Feb;126(2):794–799. [PubMed] [Google Scholar]

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