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. 1984 Mar;55(3):541–545.

Thymus-dependence of autoantibody responses to liver specific lipoprotein in the mouse.

W N Bartholomaeus, H O'Donoghue, W D Reed
PMCID: PMC1535937  PMID: 6323073

Abstract

Proposed mechanisms for the induction of autoantibodies to liver specific lipoprotein (LSP) assume that the autoantibody response is T-dependent. This hypothesis was tested in the athymic nude mouse. Athymic homozygote (nu/nu) nude mice and appropriate control mouse strains were immunized with rabbit or human LSP and infected with murine cytomegalovirus (MCMV) in an attempt to induce autoantibodies to LSP. Antibodies to LSP were measured by passive haemagglutination and by an enzyme linked immunosorbent assay. Nude mice did not produce antibodies to either foreign LSP species or autoantibodies to mouse LSP when immunized with either rabbit or human LSP. Control heterozygote (nu/+) mice and C57BL/6J mice produced antibody to foreign LSP and autoantibody to mouse LSP when immunized with xenogeneic LSP. Athymic nu/nu mice also failed to produce autoantibody to mouse LSP following infection with MCMV, in contrast to control nu/+ and C57BL/6J mice which produced LSP autoantibody after infection with MCMV. It is concluded that the autoantibody response to LSP is T-dependent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allan J. E., Shellam G. R., Grundy J. E. Effect of murine cytomegalovirus infection on mitogen responses in genetically resistant and susceptible mice. Infect Immun. 1982 Apr;36(1):235–242. doi: 10.1128/iai.36.1.235-242.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allison A. C. Unresponsiveness to self antigens. Lancet. 1971 Dec 25;2(7739):1401–1403. doi: 10.1016/s0140-6736(71)90673-8. [DOI] [PubMed] [Google Scholar]
  3. Andersen P., Andersen H. K. Smooth-muscle antibodies and other tissue antibodies in cytomegalovirus infection. Clin Exp Immunol. 1975 Oct;22(1):22–29. [PMC free article] [PubMed] [Google Scholar]
  4. Bartholomaeus W. N., Reed W. D., Joske R. A., Shilkin K. B. Autoantibody responses to liver-specific lipoprotein in mice. Immunology. 1981 Jun;43(2):219–226. [PMC free article] [PubMed] [Google Scholar]
  5. Bartholomaeus W. N., Shellam G. R., Allan J. E., Reed W. D., Joske R. A. Autoantibodies to liver-specific lipoprotein following hepatitis induced by mouse cytomegalovirus. Clin Exp Immunol. 1983 Apr;52(1):89–97. [PMC free article] [PubMed] [Google Scholar]
  6. Chalmer J. E., Mackenzie J. S., Stanley N. F. Resistance to murine cytomegalovirus linked to the major histocompatibility complex of the mouse. J Gen Virol. 1977 Oct;37(1):107–114. doi: 10.1099/0022-1317-37-1-107. [DOI] [PubMed] [Google Scholar]
  7. Cunningham A. J. Self-tolerance maintained by active suppressor mechanisms. Transplant Rev. 1976;31:23–43. doi: 10.1111/j.1600-065x.1976.tb01451.x. [DOI] [PubMed] [Google Scholar]
  8. Eddleston A. L., Williams R. Inadequate antibody response to hBAg or suppressor T-cell defect in development of active chronic hepatitis. Lancet. 1974 Dec 28;2(7896):1543–1545. doi: 10.1016/s0140-6736(74)90287-6. [DOI] [PubMed] [Google Scholar]
  9. Howard R. J., Miller J., Najarian J. S. Cytomegalovirus-induced immune suppression. II. Cell-mediated immunity. Clin Exp Immunol. 1974 Sep;18(1):119–126. [PMC free article] [PubMed] [Google Scholar]
  10. Howard R. J., Najarian J. S. Cytomegalovirus-induced immune suppression. I. Humoral immunity. Clin Exp Immunol. 1974 Sep;18(1):109–118. [PMC free article] [PubMed] [Google Scholar]
  11. Jensen D. M., McFarlane I. G., Portmann B. S., Eddleston A. L., Williams R. Detection of antibodies directed against a liver-specific membrane lipoprotein in patients with acute and chronic active hepatitis. N Engl J Med. 1978 Jul 6;299(1):1–7. doi: 10.1056/NEJM197807062990101. [DOI] [PubMed] [Google Scholar]
  12. Manns M., Meyer zum Büschenfelde K. H., Hess G. Autoantibodies against liver-specific membrane lipoprotein in acute and chronic liver diseases: studies on organ-, species-, and disease-specificity. Gut. 1980 Nov;21(11):955–961. doi: 10.1136/gut.21.11.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McFarlane I. G., Wojcicka B. M., Zucker G. M., Eddleston A. L., Williams R. Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol. 1977 Mar;27(3):381–390. [PMC free article] [PubMed] [Google Scholar]
  14. Meliconi R., Perperas A., Jensen D., Alberti A., McFarlane I. G., Eddleston A. L., Williams R. Anti-LSP antibodies in acute liver disease. Gut. 1982 Jul;23(7):603–607. doi: 10.1136/gut.23.7.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Olding L. B., Kingsbury D. T., Oldstone M. B. Pathogenesis of cytomegalovirus infection. Distribution of viral products, immune complexes and autoimmunity during latent murine infection. J Gen Virol. 1976 Nov;33(2):267–280. doi: 10.1099/0022-1317-33-2-267. [DOI] [PubMed] [Google Scholar]
  16. Starr S. E., Allison A. C. Role of T lymphocytes in recovery from murine cytomegalovirus infection. Infect Immun. 1977 Aug;17(2):458–462. doi: 10.1128/iai.17.2.458-462.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tinghitella T. J., Booss J. Enhanced immune response late in primary cytomegalovirus infection of mice. J Immunol. 1979 Jun;122(6):2442–2446. [PubMed] [Google Scholar]
  18. Wager O., Räsänen J. A., Hagman A., Klemola E. Mixed cryoimmunoglobulinaemia in infectious mononucleois and Cytomegalovirus mononucleosis. Int Arch Allergy Appl Immunol. 1968;34(4):345–361. doi: 10.1159/000230129. [DOI] [PubMed] [Google Scholar]
  19. Weigle W. O. Recent observations and concepts in immunological unresponsiveness and autoimmunity. Clin Exp Immunol. 1971 Oct;9(4):437–447. [PMC free article] [PubMed] [Google Scholar]

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