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. 1984 Apr;56(1):175–184.

Immunological studies of human placentae: complement components in pre-eclamptic chorionic villi.

D Sinha, M Wells, W P Faulk
PMCID: PMC1535962  PMID: 6370518

Abstract

Forty human placentae were studied by immunofluorescence for the presence of complement (C) components C1q, C4, C3d, C6 and C9 with the use of characterized antisera. The tissues were grouped as control placentae from 20 normal pregnancies and 20 from cases of pre-eclampsia (PE): the PE samples were sub-grouped as being obtained from patients with mild or severe PE. All of the C components studied were found in the same distribution for test and control samples, but statistical analysis of each pattern of distribution revealed that the deposition of C1q, C3d and C9 were increased in PE as compared to normal tissues. This impression was strengthened by the finding that the deposition of these C components was further increased when chorionic villus immunopathology was compared between mild and severe PE. These data indicate that immunological mechanisms are operating in PE chorionic villi, and they suggest that, among other mechanisms, immune processes may be operative in the pathophysiology of this clinical disease, and that more studies along these lines are in order to rule either in or out this possibility.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bokisch V. A., Dierich M. P., Mūller-Eberhard H. J. Third component of complement (C3): structural properties in relation to functions. Proc Natl Acad Sci U S A. 1975 Jun;72(6):1989–1993. doi: 10.1073/pnas.72.6.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Faulk W. P., Hijmans W. Recent developments in immunofluorescence. Prog Allergy. 1972;16:9–39. doi: 10.1159/000393067. [DOI] [PubMed] [Google Scholar]
  3. Faulk W. P., Jarret R., Keane M., Johnson P. M., Boackle R. J. Immunological studies of human placentae: complement components in immature and mature chorionic villi. Clin Exp Immunol. 1980 May;40(2):299–305. [PMC free article] [PubMed] [Google Scholar]
  4. Faulk W. P., Johnson P. M. Immunological studies of human placentae: identification and distribution of proteins in mature chorionic villi. Clin Exp Immunol. 1977 Feb;27(2):365–375. [PMC free article] [PubMed] [Google Scholar]
  5. Faulk W. P., McIntyre J. A. Immunological studies of human trophoblast: markers, subsets and functions. Immunol Rev. 1983;75:139–175. doi: 10.1111/j.1600-065x.1983.tb01094.x. [DOI] [PubMed] [Google Scholar]
  6. Faulk W. P., McIntyre J. A. Trophoblast survival. Transplantation. 1981 Jul;32(1):1–5. [PubMed] [Google Scholar]
  7. Faulk W. P., Pondman K. W. Characterization of rabbit fast and slow 6-6S IgG. Immunochemistry. 1969 Mar;6(2):215–228. doi: 10.1016/0019-2791(69)90158-x. [DOI] [PubMed] [Google Scholar]
  8. Faulk W. P., Temple A. Distribution of beta2 microglobulin and HLA in chorionic villi of human placentae. Nature. 1976 Aug 26;262(5571):799–802. doi: 10.1038/262799a0. [DOI] [PubMed] [Google Scholar]
  9. Faulk W. P., Temple A., Lovins R. E., Smith N. Antigens of human trophoblasts: a working hypothesis for their role in normal and abnormal pregnancies. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1947–1951. doi: 10.1073/pnas.75.4.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Faulk W. P., van Loghem E., Stickler G. B. Maternal antibody to fetal light chain (Inv) antigens. Am J Med. 1974 Mar;56(3):393–397. doi: 10.1016/0002-9343(74)90621-4. [DOI] [PubMed] [Google Scholar]
  11. Gaugas J. M., Curzen P. Complement fixing antibody against solubilized placental microsomal fraction in pre-eclampsia sera. Br J Exp Pathol. 1974 Dec;55(6):570–574. [PMC free article] [PubMed] [Google Scholar]
  12. IKLE F. A. DISSEMINATION VON SYNCYTIOTROPHOBLASTZELLEN IM MUETTERLICHEN BLUT WAEHREND DER GRAVIDITAET. Bull Schweiz Akad Med Wiss. 1964 Mar;20:62–72. [PubMed] [Google Scholar]
  13. Jeannet M., Werner C., Ramirez E., Vassalli P., Faulk W. P. Anti-HLA, anti-human "Ia-like" and MLC blocking activity of human placental IgG. Transplant Proc. 1977 Jun;9(2):1417–1422. [PubMed] [Google Scholar]
  14. Johnson P. M., Faulk W. P. Immunological studies of human placentae: identification and distribution of proteins in immature chorionic villi. Immunology. 1978 Jun;34(6):1027–1035. [PMC free article] [PubMed] [Google Scholar]
  15. Johnson P. M., Natvig J. B., Ystehede U. A., Faulk W. P. Immunological studies of human placentae: the distribution and character of immunoglobulins in chorionic villi. Clin Exp Immunol. 1977 Oct;30(1):145–153. [PMC free article] [PubMed] [Google Scholar]
  16. Jämeri K. E., Koivuniemi A. P., Carpén E. O. Occurrence of trophoblasts in the blood of toxaemic patients. Gynaecologia. 1965;160(5):315–320. doi: 10.1159/000303403. [DOI] [PubMed] [Google Scholar]
  17. Linder E. Binding of C1q and complement activation by vascular endothelium. J Immunol. 1981 Feb;126(2):648–658. [PubMed] [Google Scholar]
  18. MACGILLIVRAY I. Hypertension in pregnancy and its consequences. J Obstet Gynaecol Br Commonw. 1961 Aug;68:557–569. doi: 10.1111/j.1471-0528.1961.tb02771.x. [DOI] [PubMed] [Google Scholar]
  19. Masson P. L., Delire M., Cambiaso C. L. Circulating immune complexes in normal human pregnancy. Nature. 1977 Apr 7;266(5602):542–543. doi: 10.1038/266542a0. [DOI] [PubMed] [Google Scholar]
  20. McCormick J. N., Faulk W. P., Fox H., Fudenberg H. H. Immunohistological and elution studies of the human placenta. J Exp Med. 1971 Jan 1;133(1):1–18. doi: 10.1084/jem.133.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McIntyre J. A., Faulk W. P. Trophoblast modulation of maternal allogeneic recognition. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4029–4032. doi: 10.1073/pnas.76.8.4029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. NELSON T. R. A clinical study of pre-eclampsia. I. J Obstet Gynaecol Br Emp. 1955 Feb;62(1):48–57. doi: 10.1111/j.1471-0528.1955.tb14094.x. [DOI] [PubMed] [Google Scholar]
  23. O'Sullivan M. J., McIntyre J. A., Prior M., Warriner G., Faulk W. P. Identification of human trophoblast membrane antigens in maternal blood during pregnancy. Clin Exp Immunol. 1982 Apr;48(1):279–287. [PMC free article] [PubMed] [Google Scholar]
  24. Redman C. W., Bodmer J. G., Bodmer W. F., Beilin L. J., Bonnar J. HLA antigens in severe pre-eclampsia. Lancet. 1978 Aug 19;2(8086):397–399. doi: 10.1016/s0140-6736(78)91866-4. [DOI] [PubMed] [Google Scholar]
  25. Robertson W. B., Brosens I., Dixon H. G. The pathological response of the vessels of the placental bed to hypertensive pregnancy. J Pathol Bacteriol. 1967 Apr;93(2):581–592. doi: 10.1002/path.1700930219. [DOI] [PubMed] [Google Scholar]
  26. Robertson W. B. Uteroplacental vasculature. J Clin Pathol Suppl (R Coll Pathol) 1976;10:9–17. doi: 10.1136/jcp.s3-10.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Scott J. S., Jenkins D. M., Need J. A. Immunology of pre-eclampsia. Lancet. 1978 Apr 1;1(8066):704–706. doi: 10.1016/s0140-6736(78)90813-9. [DOI] [PubMed] [Google Scholar]
  28. Stirrat G. M., Redman C. W., Levinsky R. J. Circulating immune complexes in pre-eclampsia. Br Med J. 1978 Jun 3;1(6125):1450–1451. doi: 10.1136/bmj.1.6125.1450. [DOI] [PMC free article] [PubMed] [Google Scholar]

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