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. 1984 Apr;56(1):58–66.

Polyclonal B cell activation, circulating immune complexes and autoimmunity in human american visceral leishmaniasis.

B Galvão-Castro, J A Sá Ferreira, K F Marzochi, M C Marzochi, S G Coutinho, P H Lambert
PMCID: PMC1535977  PMID: 6424987

Abstract

In a prospective study, serum and plasma samples from 17 patients with kala-azar were collected in Rio de Janeiro. High levels of immune complexes (IC) were detected in serum by means of 125I-Clq and conglutinin binding assays. The Clq binding material had a sedimentation coefficient of 19-25S, as determined by ultracentrifugation on sucrose gradient. Plasma levels of C3 and C3 breakdown products were measured and the C3d levels were increased in six out of 11 patients. The occurrence of polyclonal B cell activation was suggested by (a) a marked increase of serum IgG and IgM levels and (b) of the presence of antibodies against various proteins and haptens (SRBC, DNP-BSA, FITC-BSA,KLH). There was a close association between the presence of IC and anti-immunoglobulin antibodies. Anti-smooth muscle antibodies were also observed. These data are consistent with a major role of polyclonal B cell activation in the induction of IC during visceral leishmaniasis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrade Z. A., Iabuki K. A nefropatía do calazar. Rev Inst Med Trop Sao Paulo. 1972 Jan-Feb;14(1):51–54. [PubMed] [Google Scholar]
  2. Campos-Neto A., Bunn-Moreno M. M. Polyclonal B cell activation in hamsters infected with parasites of the genus Leishmania. Infect Immun. 1982 Dec;38(3):871–876. doi: 10.1128/iai.38.3.871-876.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carvalho E. M., Andrews B. S., Martinelli R., Dutra M., Rocha H. Circulating immune complexes and rheumatoid factor in schistosomiasis and visceral leishmaniasis. Am J Trop Med Hyg. 1983 Jan;32(1):61–68. doi: 10.4269/ajtmh.1983.32.61. [DOI] [PubMed] [Google Scholar]
  4. Carvalho E. M., Teixeira R. S., Johnson W. D., Jr Cell-mediated immunity in American visceral leishmaniasis: reversible immunosuppression during acute infection. Infect Immun. 1981 Aug;33(2):498–500. doi: 10.1128/iai.33.2.498-500.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casali P., Bossus A., Carpentier N. A., Lambert P. H. Solid-phase enzyme immunoassay or radioimmunoassay for the detection of immune complexes based on their recognition by conglutinin: conglutinin-binding test. A comparative study with 125I-labelled Clq binding and Raji-cell RIA tests. Clin Exp Immunol. 1977 Aug;29(2):342–354. [PMC free article] [PubMed] [Google Scholar]
  6. Casali P., Lambert P. H. Purification of soluble immune complexes from serum using polymethylmetacrylate beads coated with conglutinin or C1q. Application to the analysis of the components of in vitro formed immune complexes and of immune complexes occurring in vivo during leishmaniasis. Clin Exp Immunol. 1979 Aug;37(2):295–309. [PMC free article] [PubMed] [Google Scholar]
  7. Chavas J., Guimarães Ferri R. Immunoglobulins in visceral leishmaniasis. Rev Inst Med Trop Sao Paulo. 1966 Sep-Oct;8(5):225–226. [PubMed] [Google Scholar]
  8. Chiller J. M., Weigle W. O. Termination of tolerance to human gamma globulin in mice by antigen and bacterial lipopolysaccharide (endotoxin). J Exp Med. 1973 Mar 1;137(3):740–750. doi: 10.1084/jem.137.3.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DE Brito T., Hoshino-Shimizu S., Neto V. A., Duarte I. S., Penna D. O. Glomerular involvement in human kala-azar. A light, immunofluorescent, and electron microscopic study based on kidney biopsies. Am J Trop Med Hyg. 1975 Jan;24(1):9–18. [PubMed] [Google Scholar]
  10. Diamantstein T., Keppler W., Blitstein-Willinger E. Suppression of the primary immune response in vivo to sheep red blood cells by B-cell mitogens. Immunology. 1976 Mar;30(3):401–407. [PMC free article] [PubMed] [Google Scholar]
  11. Goldman M., Rose L. M., Hochmann A., Lambert P. H. Deposition of idiotype-anti-idiotype immune complexes in renal glomeruli after polyclonal B cell activation. J Exp Med. 1982 May 1;155(5):1385–1399. doi: 10.1084/jem.155.5.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  13. Ho M., Koech D. K., Iha D. W., Bryceson A. D. Immunosuppression in Kenyan visceral leishmaniasis. Clin Exp Immunol. 1983 Feb;51(2):207–214. [PMC free article] [PubMed] [Google Scholar]
  14. Izui S., Kobayakawa T., Louis J., Lambert P. H. Induction of thymocytotoxic autoantibodies after injection of bacterial lipopolysaccharides in mice. Eur J Immunol. 1979 Apr;9(4):338–341. doi: 10.1002/eji.1830090416. [DOI] [PubMed] [Google Scholar]
  15. Izui S., Kobayakawa T., Zryd M. J., Louis J., Lambert P. H. Mechanism for induction of anti-DNA antibodies by bacterial lipopolysaccharides in mice; II. Correlation between anti-DNA induction and polyclonal antibody formation by various polyclonal B lymphocyte activators. J Immunol. 1977 Dec;119(6):2157–2162. [PubMed] [Google Scholar]
  16. Izui S., Lambert P. H., Miescher P. A. Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent. Clin Exp Immunol. 1976 Dec;26(3):425–430. [PMC free article] [PubMed] [Google Scholar]
  17. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  18. Mannik M., Arend M. P., Hall A. P., Gilliland B. C. Studies on antigen-antibody complexes. I. Elimination of soluble complexes from rabbit circulation. J Exp Med. 1971 Apr 1;133(4):713–739. doi: 10.1084/jem.133.4.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. ODDO F. G., CASCIO G. IL TEST DI IMMUNO-FLUORESCENZA NELLE LEISHMANIOSI VISCERALE E CUTANEA. Riv Ist Sieroter Ital. 1963 May-Jun;38:139–145. [PubMed] [Google Scholar]
  20. Perrin L. H., Lambert P. H., Miescher P. A. Complement breakdown products in plasma from patients with systemic lupus erythematosus and patients with membranoproliferative or other glomerulonephritis. J Clin Invest. 1975 Jul;56(1):165–176. doi: 10.1172/JCI108065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pugin P., Miescher P. A. Le kala-azar. Etude clinique et physiopathologique à propos d'un nouveau cas observé en Suisse. Schweiz Med Wochenschr. 1979 Feb 24;109(8):265–269. [PubMed] [Google Scholar]
  22. Ramos-Niembro F., Fournié G., Lambert P. H. Induction of circulating immune complexes and their renal localization after acute or chronic polyclonal B-cell activation in mice. Kidney Int Suppl. 1982 May;11:S29–S38. [PubMed] [Google Scholar]
  23. Rose L. M., Goldman M., Lambert P. H. Simultaneous induction of an idiotype, corresponding anti-idiotypic antibodies, and immune complexes during African trypanosomiasis in mice. J Immunol. 1982 Jan;128(1):79–85. [PubMed] [Google Scholar]
  24. Rosenberg Y. J. Autoimmune and polyclonal B cell responses during murine malaria. Nature. 1978 Jul 13;274(5667):170–172. doi: 10.1038/274170a0. [DOI] [PubMed] [Google Scholar]
  25. Santoro F., Vandemeulebrouke B., Capron A. The use of the radioimmunoprecipitation-PEG assay (RIPEGA) to quantify circulating antigens in human and experimental schistosomiasis. J Immunol Methods. 1978;24(3-4):229–237. doi: 10.1016/0022-1759(78)90127-8. [DOI] [PubMed] [Google Scholar]
  26. Sehgal S., Aikat B. K., Pathania A. G. Immune complexes in Indian kala-azar. Bull World Health Organ. 1982;60(6):945–950. [PMC free article] [PubMed] [Google Scholar]
  27. Szarfman A., Cossio P. M., Diez C., Arana R. M., Sadun E. Antibodies against endocardium, vascular structures, and interstitium of striated muscle that cross-react with T. cruzi and T. rhodesiense. J Parasitol. 1974 Dec;60(6):1024–1024. [PubMed] [Google Scholar]
  28. Thomas H. C., De Villiers D., Potter B., Hodgson H., Jain S., Jewell D. P., Sherlock S. Immune complexes in acute and chronic liver disease. Clin Exp Immunol. 1978 Feb;31(2):150–157. [PMC free article] [PubMed] [Google Scholar]
  29. Weintraub J., Gottlieb M., Weinbaum F. I. Leishmania tropica: association of a B-cell mitogen with hypergammaglobulinemia in mice. Exp Parasitol. 1982 Feb;53(1):87–96. doi: 10.1016/0014-4894(82)90095-9. [DOI] [PubMed] [Google Scholar]
  30. Wyler D. J. Circulating factor from a kala-azar patient suppresses in vitro antileishmanial T cell proliferation. Trans R Soc Trop Med Hyg. 1982;76(3):304–306. doi: 10.1016/0035-9203(82)90174-2. [DOI] [PubMed] [Google Scholar]
  31. Zubler R. H., Lange G., Lambert P. H., Miescher P. A. Detection of immune complexes in unheated sera by modified 125I-Clq binding test. Effect of heating on the binding of Clq by immune complexes and application of the test to systemic lupus erythematosus. J Immunol. 1976 Jan;116(1):232–235. [PubMed] [Google Scholar]

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