Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1983 Jun;52(3):472–476.

Diminished active T rosette levels and increased spontaneous B lymphocyte blastogenesis in hepatitis B virus positive chronic active hepatitis.

G Budillon, G Scala, C D'Onofrio, S Cassano, F De Ritis
PMCID: PMC1536045  PMID: 6603293

Abstract

The present investigation was designed to detect abnormalities in CMI and the presence of polyclonally activated B cells in patients with HBV positive CAH. We studied the peripheral levels and 3H-thymidine incorporation of three lymphocyte subsets: B lymphocytes, as well as two T cell subsets that are either active or late rosetters with high and low affinity receptors respectively for sheep red blood cells (SRBC). In patients the level of peripheral T active cells was decreased, but they exhibited elevated B cell activation. There was also a significant correlation between the decreased levels of T active cells and increased 3H-thymidine incorporation by B lymphocytes. Taken together, our results are consistent with the hypothesis that patients with HBV positive CAH have a severe impairment of T cell function that may lead to an abnormal B cell activation. The increased B cell activity may account for the presence of circulating immune complexes and the variety of autoantibodies often observed in patients with HBV positive CAH.

Full text

PDF
472

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chisari F. V., Bieber M. S., Josepho C. A., Xavier C., Anderson D. S. Functional properties of lymphocyte subpopulations in hepatitis B virus infection. II. Cytotoxic effector cell killing of targets that naturally express hepatitis B surface antigen and liver-specific lipoprotein. J Immunol. 1981 Jan;126(1):45–49. [PubMed] [Google Scholar]
  2. Colombo M., Vernace S. J., Paronetto F. T and B lymphocytes in patients with chronic active hepatitis (CAH). Clin Exp Immunol. 1977 Oct;30(1):4–9. [PMC free article] [PubMed] [Google Scholar]
  3. De Bruyere M., Lachapelle J. M., Anguita T. Spontaneous 3H-thymidine incorporation by circulating lymphocytes of normal subjects: relative proportion of T and B cells. Int Arch Allergy Appl Immunol. 1977;54(1):50–57. doi: 10.1159/000231807. [DOI] [PubMed] [Google Scholar]
  4. Dienstag J. L., Isselbacher K. J. Liver-specific protein: more questions than answers. N Engl J Med. 1978 Jul 6;299(1):40–42. doi: 10.1056/NEJM197807062990110. [DOI] [PubMed] [Google Scholar]
  5. Galant S. P., Lundak R. L., Eaton L. Enhancement of early human E rosette formation by cholinergic stimuli. J Immunol. 1976 Jul;117(1):48–51. [PubMed] [Google Scholar]
  6. Hanjan S. N., Talwar G. P., Kidwai Z., Nath I. Delineation and quantitation of human peripheral blood lymphocyte subpopulations by electrophoretic mobility and role of surface charge in cell to cell interaction. J Immunol. 1977 Jan;118(1):235–241. [PubMed] [Google Scholar]
  7. Horowitz S., Groshong T., Albrecht R., Hong R. The "active" rosette test in immunodeficiency diseases. Clin Immunol Immunopathol. 1975 Sep;4(3):405–414. doi: 10.1016/0090-1229(75)90009-4. [DOI] [PubMed] [Google Scholar]
  8. Hotta R., Kuriki J., Kakumu S. Loss of suppressor T cell function and circulating immune complexes in chronic active liver diseases. Clin Exp Immunol. 1981 Nov;46(2):375–381. [PMC free article] [PubMed] [Google Scholar]
  9. Keystone J. S., Murdoch J. K. Mebendazole. Ann Intern Med. 1979 Oct;91(4):582–586. doi: 10.7326/0003-4819-91-4-582. [DOI] [PubMed] [Google Scholar]
  10. Koller C. A., King G. W., Hurtubise P. E., Sagone A. L., LoBuglio A. F. Characterization of glass adherent human mononuclear cells. J Immunol. 1973 Nov;111(5):1610–1612. [PubMed] [Google Scholar]
  11. Lee W. M., Reed W. D., Mitchell C. G., Galbraith R. M., Eddleston A. L., Zuckerman A. J., Williams R. Cellular and humoral immunity to hepatitis-B surface antigen in active chronic hepatitis. Br Med J. 1975 Mar 29;1(5960):705–708. doi: 10.1136/bmj.1.5960.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Meyer zum Büschenfelde K. H., Manns M., Hütteroth T. H., Hopf U., Arnold W. LM-Ag and LSP--two different target antigens involved in the immunopathogenesis of chronic active hepatitis? Clin Exp Immunol. 1979 Aug;37(2):205–212. [PMC free article] [PubMed] [Google Scholar]
  13. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4061–4065. doi: 10.1073/pnas.76.8.4061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Taniguchi N., Miyawaki T., Moriya N., Nagaoki T., Kato E. Mitogenic responsiveness and monocyte-lymphocyte interaction of early and late rosette-forming cell populations of human peripheral blood lymphocytes. J Immunol. 1977 Jan;118(1):193–197. [PubMed] [Google Scholar]
  15. Wands K. R., Isselbacher K. J. Lymphocyte cytotoxicity to autologous liver cells in chronic active hepatitis. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1301–1303. doi: 10.1073/pnas.72.4.1301. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES