Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1984 Jul;57(1):171–177.

Diffusible suppressor factor from splenic macrophages in murine plasmacytoma.

Y H Chen, P Heller
PMCID: PMC1536073  PMID: 6378461

Abstract

The immunosuppressive effect of splenic macrophages (M phi) in mice bearing plasmacytoma was previously shown to be mediated by a diffusible factor. This diffusible suppressor factor (DSF) was found to be non-dialysable and sensitive to heating to 56 degrees C and to the proteolytic action of trypsin. The suppressor factor could be removed from culture supernatants by binding to ligands that specifically bind to corresponding myeloma proteins. DSF from splenic suppressor M phi of mice bearing MOPC 315 was capable of binding dinitrophenyl L-lysine, and that from mice bearing MOPC 104E, dextran S. The suppressor factor apparently cross-reacted with anti-idiotypic antibody to the corresponding myeloma protein, but did not interact with anti-isotypic antibody to mouse immunoglobulins (Ig). A higher concentration of mouse Ig than that found in DSF preparations did not have a suppressive effect. Metabolic inhibitors for RNA and protein, but not DNA synthesis effectively blocked the production of DSF. These findings suggest that DSF is a non-Ig protein that may have a structural similarity to myeloma idiotype. Continuous RNA and protein synthesis is required for the elaboration of DSF by splenic suppressor M phi in cultures.

Full text

PDF
171

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Broder S., Humphrey R., Durm M., Blackman M., Meade B., Goldman C., Strober W., Waldmann T. Impaired synthesis of polyclonal (non-paraprotein) immunoglobulins by circulating lymphocytes from patients with multiple myeloma Role of suppressor cells. N Engl J Med. 1975 Oct 30;293(18):887–892. doi: 10.1056/NEJM197510302931801. [DOI] [PubMed] [Google Scholar]
  2. Chen Y. H., Heller P. Suppressor activity of splenic macrophages in murine plasmacytoma (PC) is inhibited by PC specific ligands. Clin Exp Immunol. 1982 Nov;50(2):366–373. [PMC free article] [PubMed] [Google Scholar]
  3. Chen Y., Heller P. The mode of action of splenic suppressor cells in murine plasmacytoma. Clin Exp Immunol. 1981 Sep;45(3):514–522. [PMC free article] [PubMed] [Google Scholar]
  4. Chen Y., Yakulis V., Heller P. Passive immunity to murine plasmacytoma by rabbit antiidiotypic antibody to myeloma protein. Proc Soc Exp Biol Med. 1976 Jan;151(1):121–125. doi: 10.3181/00379727-151-39157. [DOI] [PubMed] [Google Scholar]
  5. JERNE N. K., NORDIN A. A. Plaque formation in agar by single antibody-producing cells. Science. 1963 Apr 26;140(3565):405–405. [PubMed] [Google Scholar]
  6. Joskowicz M., Rabourdin-Combe C., Neauport-Sautes C., Fridman W. H. Characterization of suppressive immunoglobulin-binding factor (IBF). III. Biochemical and immunochemical characteristics of IBF produced by activated T cells. J Immunol. 1978 Aug;121(2):777–783. [PubMed] [Google Scholar]
  7. Kamo I., Patel C., Kateley J., Friedman H. Immunosuppression induced in vitro by mastocytoma tumor cells and cell-free extracts. J Immunol. 1975 Jun;114(6):1749–1756. [PubMed] [Google Scholar]
  8. Kennard J., Zolla-Pazner S. Origin and function of suppressor macrophages in myeloma. J Immunol. 1980 Jan;124(1):268–273. [PubMed] [Google Scholar]
  9. Kolb J. P., Arrian S., Zolla-Pazner S. Suppression of the humoral immune response by plasmacytomas: mediation by adherent mononuclear cells. J Immunol. 1977 Feb;118(2):702–709. [PubMed] [Google Scholar]
  10. Lei H. Y., Ju S. T., Dorf M. E., Waltenbaugh C. Regulation of immune responses by I-J gene products. III. GT-specific suppressor factor is composed of separate I-J and idiotype-bearing chains. J Immunol. 1983 Mar;130(3):1274–1279. [PubMed] [Google Scholar]
  11. Lindström F. D., Hardy W. R., Eberle B. J., Williams R. C., Jr Multiple myeloma and benign monoclonal gammopathy: differentiation by immunofluorescence of lymphocytes. Ann Intern Med. 1973 Jun;78(6):837–844. doi: 10.7326/0003-4819-78-6-837. [DOI] [PubMed] [Google Scholar]
  12. Marbrook J. Primary immune response in cultures of spleen cells. Lancet. 1967 Dec 16;2(7529):1279–1281. doi: 10.1016/s0140-6736(67)90393-5. [DOI] [PubMed] [Google Scholar]
  13. Potter M. Antigen-binding myeloma proteins of mice. Adv Immunol. 1977;25:141–211. [PubMed] [Google Scholar]
  14. Sjöberg O., Andersson J., Möller G. Requirement for adherent cells in the primary and secondary immune response in vitro. Eur J Immunol. 1972 Apr;2(2):123–126. doi: 10.1002/eji.1830020206. [DOI] [PubMed] [Google Scholar]
  15. Taniguchi M., Saito T., Takei I., Tokuhisa T. Presence of interchain disulfide bonds between two gene products that compose the secreted form of an antigen-specific suppressor factor. J Exp Med. 1981 Jun 1;153(6):1672–1677. doi: 10.1084/jem.153.6.1672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Theze J., Kapp J. A., Benacerraf B. Immunosuppressive factor(s) extracted from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) III. Immunochemical properties of the GAT-specific suppressive factor. J Exp Med. 1977 Apr 1;145(4):839–856. doi: 10.1084/jem.145.4.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yakulis V., Bhoopalam N., Schade S., Heller P. Surface immunoglobulins of circulating lymphocytes in mouse plasmacytoma. I. Characteristics of lymphocyte surface immunoglobulins. Blood. 1972 Apr;39(4):453–464. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES