Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1984 Aug;57(2):351–357.

J chain synthesis and ultrastructural changes in chronic lymphocytic leukaemia cells during in vitro differentiation.

N Yasuda, H Shimada, T Kanoh, H Uchino
PMCID: PMC1536113  PMID: 6432382

Abstract

The synthesis of J chain and the ultrastructural changes in chronic lymphocytic leukaemia (CLL) cells during in vitro cultures stimulated with PWM and/or allogenic T cells were investigated by immunoelectron microscopy, radioimmunoassay and anti-idiotype antibodies, in an attempt to demonstrate changes in the maturation stage of CLL cells. The cells from four patients showed the induction of J chain synthesis in parallel with the increase of Ig synthesis. Two of revealed cytological transformations, in which CLL cells resembled immunoblasts or plasmacytoid cells. These findings suggest that some CLL cells are not 'frozen' within the original maturation stage and can be induced to differentiate to a more mature stage.

Full text

PDF
351

Images in this article

354Fig. 3
on p.354
355Fig. 4
on p.355

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brandtzaeg P. Presence of J chain in human immunocytes containing various immunoglobulin classes. Nature. 1974 Nov 29;252(5482):418–420. doi: 10.1038/252418a0. [DOI] [PubMed] [Google Scholar]
  2. Douglas S. D., Hoffman P. F., Borjeson J., Chessin L. N. Studies on human peripheral blood lymphocytes in vitro. 3. Fine structural features of lymphocyte transformation by pokeweed mitogen. J Immunol. 1967 Jan;98(1):17–30. [PubMed] [Google Scholar]
  3. Fu S. M., Chiorazzi N., Kunkel H. G., Halper J. P., Harris S. R. Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes. J Exp Med. 1978 Dec 1;148(6):1570–1578. doi: 10.1084/jem.148.6.1570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Janossy G., Gomez De La Concha E., Waxdal M. J., Platts-Mills T. The effects of purified mitogenic proteins (Pa-1 and Pa-2) from pokeweed on human T and B lymphocytes in vitro. Clin Exp Immunol. 1976 Oct;26(1):108–117. [PMC free article] [PubMed] [Google Scholar]
  5. Lieberman M. M., Ayala E. Active and passive immunity against Pseudomonas aeruginosa with a ribosomal vaccine and antiserum in C3H/HeJ mice. J Immunol. 1983 Jul;131(1):1–3. [PubMed] [Google Scholar]
  6. Melchers F., Andersson J. IgM in bone marrow-derived lymphocytes. Changes in synthesis, turnover and secretion, and in numbers of molecules on the surface of B cells after mitogenic stimulation. Eur J Immunol. 1974 Mar;4(3):181–188. doi: 10.1002/eji.1830040306. [DOI] [PubMed] [Google Scholar]
  7. Mestecky J., Winchester R. J., Hoffman T., Kunkel H. G. Parallel synthesis of immunoglobulins and J chain in pokeweed mitogen-stimulated normal cells and in lymphoblastoid cell lines. J Exp Med. 1977 Mar 1;145(3):760–765. doi: 10.1084/jem.145.3.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Murphy M. J., Hay J. B., Morris B., Bessis M. C. Ultrastructural analysis of antibody synthesis in cells from lymph and lymph nodes. Am J Pathol. 1972 Jan;66(1):25–42. [PMC free article] [PubMed] [Google Scholar]
  9. Newell D. G., Harris A. H., Smith J. L. The ultrastructural localization of immunoglobulin in chronic lymphocytic lymphoma cells: changes in light and heavy chain distribution induced by mitogen stimulation. Blood. 1983 Mar;61(3):511–519. [PubMed] [Google Scholar]
  10. Robèrt K. H., Möller E., Gahrton G., Eriksson H., Nilsson B. B-cell activation of peripheral blood lymphocytes from patients with chronic lymphatic leukaemia. Clin Exp Immunol. 1978 Aug;33(2):302–308. [PMC free article] [PubMed] [Google Scholar]
  11. Saiki O., Kishimoto T., Kuritani T., Muraguchi A., Yamamura Y. In vitro induction of IgM secretion and switching to IgG production in human B leukemic cells with the help of T cells. J Immunol. 1980 Jun;124(6):2609–2614. [PubMed] [Google Scholar]
  12. Salmon S. E., Seligmann M. B-cell neoplasia in man. Lancet. 1974 Nov 23;2(7891):1230–1233. doi: 10.1016/s0140-6736(74)90748-x. [DOI] [PubMed] [Google Scholar]
  13. Stevenson F. K., Hamblin T. J., Stevenson G. T., Tutt A. L. Extracellular idiotypic immunoglobulin arising from human leukemic B lymphocytes. J Exp Med. 1980 Dec 1;152(6):1484–1496. doi: 10.1084/jem.152.6.1484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Yasuda N., Kanoh T., Shirakawa S., Uchino H. Intracellular immunoglobulin in lymphocytes from patients with chronic lymphocytic leukemia: an immunoelectron microscopic study. Leuk Res. 1982;6(5):659–667. doi: 10.1016/0145-2126(82)90082-0. [DOI] [PubMed] [Google Scholar]
  15. Yasuda N., Kanoh T., Uchino H. J chain synthesis in human myeloma cells: light and electron microscopic studies. Clin Exp Immunol. 1980 Jun;40(3):573–580. [PMC free article] [PubMed] [Google Scholar]
  16. Yasuda N., Kanoh T., Uchino H. J chain synthesis in lymphocytes from patients with selective IgA deficiency. Clin Exp Immunol. 1981 Oct;46(1):142–148. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES