Abstract
Rat X rat hybridomas secreting antibodies with tumour specificity have been prepared using cells from the mesenteric nodes of rats bearing syngeneic sarcomata in their Peyer's patches. The antibodies obtained, which embraced all of the major immunoglobulin classes, varied in cellular reactivity from the individually tumour specific to cross-reactive with both normal and tumour cells. Several IgA producing hybridomas were prepared using this protocol but IgG secretors were obtained more frequently and they accounted for about half of the specific hybridomas. Specific IgG producers were found to predominate also when hybridomas were prepared from the mesenteric nodes of a rat immunized by injection of horseradish peroxidase into the Peyer's patches. Comparison of these data with our earlier results using spleen cells taken from rats that were either hyperimmunized with, or were bearing the same tumours in the leg, show that mesenteric nodes draining a tumour growing in the Peyer's patches are a much better source specific IgG producing B cells.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andrew E., Hall J. G. IgA antibodies in the bile of rats. I. Some characteristics of the primary response. Immunology. 1982 Jan;45(1):169–175. [PMC free article] [PubMed] [Google Scholar]
- Currie G. A., Gage J. O. Influence of tumour growth on the evolution of cytotoxic lymphoid cells in rats bearing a spontaneously metastasizing syngeneic fibrosarcoma. Br J Cancer. 1973 Aug;28(2):136–146. doi: 10.1038/bjc.1973.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dean C. J., Gyure L. A., Styles J. M., Hobbs S. M., North S. M., Hall J. G. Production of IgA secreting hybridomas: a monoclonal rat antibody of the IgA class with specificity for RT1c. J Immunol Methods. 1982 Sep 30;53(3):307–312. doi: 10.1016/0022-1759(82)90177-6. [DOI] [PubMed] [Google Scholar]
- Eccles S. A., Heckford S. E., Alexander P. Effect of cyclosporin A on the growth and spontaneous metastasis of syngeneic animal tumours. Br J Cancer. 1980 Aug;42(2):252–259. doi: 10.1038/bjc.1980.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Galfrè G., Milstein C., Wright B. Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature. 1979 Jan 11;277(5692):131–133. doi: 10.1038/277131a0. [DOI] [PubMed] [Google Scholar]
- Gyure L. A., Dean C. J., Hall J. G., Styles J. M. Tumour-specific antibodies of the IgA class in rats after the implantation of a syngeneic tumour in the gut. Br J Cancer. 1980 Apr;41(4):640–641. doi: 10.1038/bjc.1980.108. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jarrett E. E., Bazin H. Serum immunoglobulin levels in N. brasiliensis infection. Clin Exp Immunol. 1977 Nov;30(2):330–332. [PMC free article] [PubMed] [Google Scholar]
- Mayrhofer G. Sites of synthesis and localization of IgE in rats infested with Nippostrongylus brasiliensis. Ciba Found Symp. 1977 Apr 26;(46):155–182. doi: 10.1002/9780470720288.ch8. [DOI] [PubMed] [Google Scholar]
- North S. M., Dean C. J. Monoclonal antibodies to rat sarcomata. II. A syngeneic IgG2b antibody with anti-tumour activity. Immunology. 1983 Aug;49(4):667–671. [PMC free article] [PubMed] [Google Scholar]
- North S. M., Styles J. M., Hobbs S. M., Dean C. J. Monoclonal antibodies to rat sarcomata. I. Immunization procedures and source of lymphoid cells for hybridoma production. Immunology. 1982 Nov;47(3):397–405. [PMC free article] [PubMed] [Google Scholar]
- Rose M. E., Peppard J. V., Hobbs S. M. Coccidiosis: characterization of antibody responses to infection with Eimeria nieschulzi. Parasite Immunol. 1984 Jan;6(1):1–12. doi: 10.1111/j.1365-3024.1984.tb00777.x. [DOI] [PubMed] [Google Scholar]