Abstract
Lymphocytes derived from spleens of traffic trauma victims do not appear to produce human interferon (IFN) activity, spontaneously, in vitro. However, lymphocytes derived from spleens of four ITP patients were found to produce significant amounts of human IFN activity. The IFN activity produced by the splenic lymphocytes of ITP patients was neutralized by anti-gamma-IFN antisera but not anti-alpha or anti-beta antisera. The IFN activity was found to be unstable at pH 2.0 and at 56 degrees C. Thus the human IFN activity of splenic lymphocytes is characterized as human gamma-IFN. No human IFN activity was detectable in the serum of the ITP patients and it is not known whether the splenic lymphocytes of ITP patients also produce human gamma-IFN in vivo. The observations suggest that conditions prevail in the ITP state that predispose the splenic lymphocytes to produce human gamma-IFN without stimulation by exogenously added inducer.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Armstrong J. A. Semi-micro, dye-binding assay for rabbit interferon. Appl Microbiol. 1971 Apr;21(4):723–725. doi: 10.1128/am.21.4.723-725.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brey O., Garner E. P., Wells D. Cyclic thrombocytopenia associated with multiple autoantibodies. Br Med J. 1969 Aug 16;3(5667):397–398. doi: 10.1136/bmj.3.5667.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clancy R. L., Muller H. K., Ward H. A. Immunodeficiency and grouping with thyrogastric autoimmune disease in patients with chronic idiopathic thrombocytopenic purpura. Aust N Z J Med. 1974 Jun;4(3):243–246. doi: 10.1111/j.1445-5994.1974.tb03182.x. [DOI] [PubMed] [Google Scholar]
- Conley C. L. Immunologic precursors of autoimmune hematologic disorders. Autoimmune hematologic disorders. Autoimmune hemolytic anemia and thrombocytopenic purpura. Johns Hopkins Med J. 1981 Sep;149(3):101–109. [PubMed] [Google Scholar]
- Favre H., Chatelanat F., Miescher P. A. Autoimmune hematologic diseases associated with infraclinical systemic lupus erythematosus in four patients. A human equivalent of the NZB mice. Am J Med. 1979 Jan;66(1):91–95. doi: 10.1016/0002-9343(79)90487-x. [DOI] [PubMed] [Google Scholar]
- Ho M., Armstrong J. A. Interferon. Annu Rev Microbiol. 1975;29:131–161. doi: 10.1146/annurev.mi.29.100175.001023. [DOI] [PubMed] [Google Scholar]
- Hooks J. J., Moutsopoulos H. M., Geis S. A., Stahl N. I., Decker J. L., Notkins A. L. Immune interferon in the circulation of patients with autoimmune disease. N Engl J Med. 1979 Jul 5;301(1):5–8. doi: 10.1056/NEJM197907053010102. [DOI] [PubMed] [Google Scholar]
- Huhn D., Hiller E. Graves' disease and idiopathic thrombocytopenic purpura. Arch Intern Med. 1980 Sep;140(9):1252–1253. [PubMed] [Google Scholar]
- Merigan T. C., Rand K. H., Pollard R. B., Abdallah P. S., Jordan G. W., Fried R. P. Human leukocyte interferon for the treatment of herpes zoster in patients with cancer. N Engl J Med. 1978 May 4;298(18):981–987. doi: 10.1056/NEJM197805042981801. [DOI] [PubMed] [Google Scholar]
- Preble O. T., Black R. J., Friedman R. M., Klippel J. H., Vilcek J. Systemic lupus erythematosus: presence in human serum of an unusual acid-labile leukocyte interferon. Science. 1982 Apr 23;216(4544):429–431. doi: 10.1126/science.6176024. [DOI] [PubMed] [Google Scholar]
- Stuart M. J., Tomar R. H., Miller M. L., Davey F. R. Chronic idiopathic thrombocytopenic purpura. A familial immunodeficiency syndrome? JAMA. 1978 Mar 6;239(10):939–942. [PubMed] [Google Scholar]
- Tan Y. H. Purification of human fibroblast interferon prepared from modified fibroblasts. Methods Enzymol. 1981;78(Pt A):422–430. doi: 10.1016/0076-6879(81)78152-7. [DOI] [PubMed] [Google Scholar]