Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1984 May;56(2):272–280.

Association of elevated serum glycoprotein gp70 with increased gp70 immune complex formation and accelerated lupus nephritis in autoimmune male BXSB mice.

S Izui, I Hara, L M Hang, J H Elder, P J McConahey, F J Dixon
PMCID: PMC1536233  PMID: 6733971

Abstract

BXSB male mice, which spontaneously develop a systemic lupus erythematosus (SLE) like disease, were the only strain to have a significant incidence of abnormally elevated levels of gp70 in sera. Concentrations of gp70 in some mice were more than 10 times (greater than 500 micrograms/ml) those of young BXSB and any other murine strain. The presence of high serum levels of gp70 was significantly associated with hepatic sinusoidal lymphocytosis, a high incidence of which was only observed in male BXSB mice. Serum levels of gp70-anti-gp70 immune complexes were greatly increased in mice with high levels of gp70, presumably associated with increased anti-gp70 antibody production. Such mice developed fatal glomerulonephritis significantly earlier than those with lower levels of gp70. These results suggest that (1) hepatic inflammation of unknown aetiology occurring uniquely in male BXSB mice during the course of their SLE may be responsible for the enhanced expression of serum gp70 antigen, because of its nature as an acute phase reactant and (2) enhanced expression of gp70 antigen is associated with increased formation of anti-gp70 antibodies and exacerbation of lupus nephritis in male BXSB mice.

Full text

PDF
272

Images in this article

276Fig. 1
on p.276

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews B. S., Eisenberg R. A., Theofilopoulos A. N., Izui S., Wilson C. B., McConahey P. J., Murphy E. D., Roths J. B., Dixon F. J. Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestations in several strains. J Exp Med. 1978 Nov 1;148(5):1198–1215. doi: 10.1084/jem.148.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Elder J. H., Gautsch J. W., Jensen F. C., Lerner R. A., Chused T. M., Morse H. C., Hartley J. W., Rowe W. P. Differential expression of two distinct xenotropic viruses in NZB mice. Clin Immunol Immunopathol. 1980 Mar;15(3):493–501. doi: 10.1016/0090-1229(80)90061-6. [DOI] [PubMed] [Google Scholar]
  3. Elder J. H., Jensen F. C., Bryant M. L., Lerner R. A. Polymorphism of the major envelope glycoprotein (gp70) of murine C-type viruses: virion associated and differentiation antigens encoded by a multi-gene family. Nature. 1977 May 5;267(5606):23–28. doi: 10.1038/267023a0. [DOI] [PubMed] [Google Scholar]
  4. Hara I., Izui S., Dixon F. J. Murine serum glycoprotein gp70 behaves as an acute phase reactant. J Exp Med. 1982 Feb 1;155(2):345–357. doi: 10.1084/jem.155.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hara I., Izui S., McConahey P. J., Elder J. H., Jensen F. C., Dixon F. J. Induction of high serum levels of retroviral env gene products (gp70) in mice by bacterial lipopolysaccharide. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4397–4401. doi: 10.1073/pnas.78.7.4397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Izui S., Elder J. H., McConahey P. J., Dixon F. J. Identification of retroviral gp70 and anti-gp70 antibodies involved in circulating immune complexes in NZB X NZW mice. J Exp Med. 1981 May 1;153(5):1151–1160. doi: 10.1084/jem.153.5.1151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Izui S., Fernandes G., Hara I., McConahey P. J., Jensen F. C., Dixon F. J., Good R. A. Low-calorie diet selectively reduces expression of retroviral envelope glycoprotein gp70 in sera of NZB x NZW F1 hybrid mice. J Exp Med. 1981 Oct 1;154(4):1116–1124. doi: 10.1084/jem.154.4.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Izui S., Kelley V. E., McConahey P. J., Dixon F. J. Selective suppression of retroviral gp70-anti-gp70 immune complex formation by prostaglandin E1 in murine systemic lupus erythematosus. J Exp Med. 1980 Dec 1;152(6):1645–1658. doi: 10.1084/jem.152.6.1645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Izui S., Lambert P. H., Miescher P. A. Determination of anti-DNA antibodies by a modified 125I-labelled DNA-binding test. Elimination of non-specific binding of DNA to non-immunoglobulin basic proteins by using an anionic detergent. Clin Exp Immunol. 1976 Dec;26(3):425–430. [PMC free article] [PubMed] [Google Scholar]
  10. Izui S., McConahey P. J., Clark J. P., Hang L. M., Hara I., Dixon F. J. Retroviral gp70 immune complexes in NZB x NZW F2 mice with murine lupus nephritis. J Exp Med. 1981 Aug 1;154(2):517–528. doi: 10.1084/jem.154.2.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Izui S., McConahey P. J., Theofilopoulos A. N., Dixon F. J. Association of circulating retroviral gp70-anti-gp70 immune complexes with murine systemic lupus erythematosus. J Exp Med. 1979 May 1;149(5):1099–1116. doi: 10.1084/jem.149.5.1099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lerner R. A., Wilson C. B., Villano B. C., McConahey P. J., Dixon F. J. Endogenous oncornaviral gene expression in adult and fetal mice: quantitative, histologic, and physiologic studies of the major viral glycorprotein, gp70. J Exp Med. 1976 Jan 1;143(1):151–166. doi: 10.1084/jem.143.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MARSDEN P. D., HUTT M. S., WILKS N. E., VOLLER A., BLACKMAN V., SHAH K. K., CONNOR D. H., HAMILTON P. J., BANWELL J. G., LUNN H. F. AN INVESTIGATION OF TROPICAL SPLENOMEGALY AT MULAGO HOSPITAL, KAMPALA, UGANDA. Br Med J. 1965 Jan 9;1(5427):89–92. doi: 10.1136/bmj.1.5427.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maruyama N., Lindstrom C. O. H-2-linked regulation of serum gp70 production in mice. Immunogenetics. 1983;17(5):507–521. doi: 10.1007/BF00696874. [DOI] [PubMed] [Google Scholar]
  15. Obata Y., Ikeda H., Stockert E., Boyse E. A. Relation of GIX antigen of thymocytes to envelope glycoprotein of murine leukemia virus. J Exp Med. 1975 Jan 1;141(1):188–197. doi: 10.1084/jem.141.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Obata Y., Stockert E., Boyse E. A., Tung J. S., Litman G. W. Spontaneous autoimmunization to GIX cell surface antigen in hybrid mice. J Exp Med. 1976 Aug 1;144(2):533–542. doi: 10.1084/jem.144.2.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Obata Y., Stockert E., Yamaguchi M., Boyse E. A. Source and hormone-dependence of Gix-gp70 in mouse serum. J Exp Med. 1978 Sep 1;148(3):793–798. doi: 10.1084/jem.148.3.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stockert E., Old L. J., Boyse E. A. The G-IX system. A cell surface allo-antigen associated with murine leukemia virus; implications regarding chromosomal integration of the viral genome. J Exp Med. 1971 Jun 1;133(6):1334–1355. doi: 10.1084/jem.133.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Strand M., August J. T. Oncornavirus envelope glycoprotein in serum of mice. Virology. 1976 Nov;75(1):130–144. doi: 10.1016/0042-6822(76)90012-x. [DOI] [PubMed] [Google Scholar]
  20. Tung J. S., Vitetta E. S., Fleissner E., Boyse E. A. Biochemical evidence linking the GIX thymocyte surface antigen to the gp69/71 envelope glycoprotein of murine leukemia virus. J Exp Med. 1975 Jan 1;141(1):198–205. doi: 10.1084/jem.141.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES