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. 1984 Sep;57(3):548–556.

Antibody-dependent cellular cytotoxicity of human vascular endothelium in systemic sclerosis.

C A Penning, J Cunningham, M A French, G Harrison, N R Rowell, P Hughes
PMCID: PMC1536266  PMID: 6467679

Abstract

Sera from 39 patients with systemic sclerosis were examined for a cytotoxic effect on human umbilical vein endothelium. Although none of the sera produced direct cytotoxicity of 51Cr-labelled endothelial cells, even with added complement, nine sera did produce increased 51Cr release when co-cultured with endothelial cells and normal human peripheral blood mononuclear cells. The effector cells involved in this cytotoxicity possessed Fc receptors but were non-T and non-adherent while the responsible serum factor(s) was present in IgG containing fractions. This cytotoxicity tended to occur in patients with both circulating immune complexes and precipitating antibodies to nuclear and cytoplasmic antigens who, as a group, had more severe and extensive visceral disease than those without such serological abnormalities. Control studies using sera from both 27 normal controls and 19 patients with either diabetes or extensive athero-sclerotic vascular disease failed to reveal any similar cytotoxicity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BECK J. S., ANDERSON J. R., GRAY K. G., ROWELL N. R. ANTINUCLEAR AND PRECIPITATING AUTOANTIBODIES IN PROGRESSIVE SYSTEMIC SCLEROSIS. Lancet. 1963 Dec 7;2(7319):1188–1190. [PubMed] [Google Scholar]
  2. Dau P. C., Kahaleh M. B., Sagebiel R. W. Plasmapheresis and immunosuppressive drug therapy in scleroderma. Arthritis Rheum. 1981 Sep;24(9):1128–1136. doi: 10.1002/art.1780240903. [DOI] [PubMed] [Google Scholar]
  3. Horneland M., Rekvig O. P., Jørgensen L., Hannestad K. Cultured human endothelial cells display an antigen that is recognized by certain human anti-chromatin autoantibodies. Clin Exp Immunol. 1983 Nov;54(2):373–377. [PMC free article] [PubMed] [Google Scholar]
  4. Hughes P., Cunningham J., Day M., Fitzgerald J. C., French M. A., Wright J. K., Rowell N. R. Immune complexes in systemic sclerosis; detection by C1q binding, K-cell inhibition and Raji cell radioimmunoassays. J Clin Lab Immunol. 1983 Mar;10(3):133–138. [PubMed] [Google Scholar]
  5. Hughes P., Gelsthorpe K., Doughty R. W., Rowell N. R., Rosenthal F. D., Sneddon I. B. The association of HLA-B8 with visceral disease in systemic sclerosis. Clin Exp Immunol. 1978 Mar;31(3):351–356. [PMC free article] [PubMed] [Google Scholar]
  6. Jaffe E. A., Nachman R. L., Becker C. G., Minick C. R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973 Nov;52(11):2745–2756. doi: 10.1172/JCI107470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kahaleh M. B., Leroy E. C. Endothelial injury in scleroderma. A protease mechanism. J Lab Clin Med. 1983 Apr;101(4):553–560. [PubMed] [Google Scholar]
  8. Kahaleh M. B., Sherer G. K., LeRoy E. C. Endothelial injury in scleroderma. J Exp Med. 1979 Jun 1;149(6):1326–1335. doi: 10.1084/jem.149.6.1326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MacLennan I. C. Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant Rev. 1972;13:67–90. doi: 10.1111/j.1600-065x.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  10. McCarty G. A., Rice J. R., Bembe M. L., Barada F. A., Jr Anticentromere antibody. Clinical Correlations and association with favorable prognosis in patients with scleroderma variants. Arthritis Rheum. 1983 Jan;26(1):1–7. doi: 10.1002/art.1780260101. [DOI] [PubMed] [Google Scholar]
  11. Norton W. L., Nardo J. M. Vascular disease in progressive systemic sclerosis (scleroderma). Ann Intern Med. 1970 Aug;73(2):317–324. doi: 10.7326/0003-4819-73-2-317. [DOI] [PubMed] [Google Scholar]
  12. Penning C. A., Hughes P., Rowell N. R. The production of antibody-dependent cellular cytotoxicity by immune complexes in systemic lupus erythematosus. J Clin Lab Immunol. 1984 Mar;13(3):123–127. [PubMed] [Google Scholar]
  13. Rekvig O. P., Hannestad K. The specificity of human autoantibodies that react with both cell nuclei and plasma membranes: the nuclear antigen is present on core mononucleosomes. J Immunol. 1979 Dec;123(6):2673–2681. [PubMed] [Google Scholar]
  14. Tan E. M., Rodnan G. P., Garcia I., Moroi Y., Fritzler M. J., Peebles C. Diversity of antinuclear antibodies in progressive systemic sclerosis. Anti-centromere antibody and its relationship to CREST syndrome. Arthritis Rheum. 1980 Jun;23(6):617–625. doi: 10.1002/art.1780230602. [DOI] [PubMed] [Google Scholar]
  15. Yanase K., Imamura S. Detection of circulating immune complexes in some skin diseases by platelet aggregation test. Br J Dermatol. 1979 Feb;100(2):227–228. doi: 10.1111/j.1365-2133.1979.tb05567.x. [DOI] [PubMed] [Google Scholar]
  16. van der Maulen J., Wouda A. A., Mandema E., The T. H. Immune complexes in peripheral blood polymorphonuclear leucocytes of patients with Raynaud's phenomenon. Clin Exp Immunol. 1979 Jan;35(1):62–66. [PMC free article] [PubMed] [Google Scholar]

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