Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1981 Dec;46(3):565–574.

Autoantibody against fibroblasts in renal transplant recipients: analysis by 51Cr-release assays.

M Aprile, P Halloran, G Haddad, M Robinette
PMCID: PMC1536313  PMID: 7039893

Abstract

In testing for donor-specific alloantibody in renal transplantation we discovered that sera of many recipients lysed their own fibroblasts in complement-dependent cytotoxicity. The incidence of this autoreactivity before and during the first month post-transplant was 15 and 10% respectively, but rose to 48% after 2 months. There was a significant association between autoantibody formation and the presence of donor-specific alloantibody, but no association between autoantibody and graft survival. Autoantibody reactive with fibroblasts was three times more frequent than lymphocytotoxic autoantibody, and showed no association with the presence of such antibody, indicating that these autoantibodies were directed against different antigens. Reduction with 2-mercaptoethanol indicated that much of the autoreactivity was IgM. By absorption tests the autoantigen was shown to be expressed extensively on fibroblasts, but not on lymphocytes, platelets or red cells. Susceptibility to lysis by the autoantibody varied from day to day in the fibroblasts of a particular individual, and also varied from individual to individual. These results are of interest because they indicate that autoantibody formation in human renal allografts may result from chronic allogeneic stimulation, and that the antibody may be detecting a previously unknown fibroblast antigen.

Full text

PDF
565

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ayoub G., Park M. S., Terasaki P. I., Iwaki Y., Opelz G. B cell antibodies and crossmatching. Transplantation. 1980 Mar;29(3):227–229. doi: 10.1097/00007890-198003000-00013. [DOI] [PubMed] [Google Scholar]
  2. Chalopin J. M., Rifle G. Role of cold lymphocytotoxic antibodies (CLA) in human allograft recipients. Transplant Proc. 1979 Jun;11(2):1271–1273. [PubMed] [Google Scholar]
  3. Cross D. E., Greiner R., Whittier F. C. Importance of the autocontrol crossmatch in human renal transplantation. Transplantation. 1976 Apr;21(4):307–311. doi: 10.1097/00007890-197604000-00006. [DOI] [PubMed] [Google Scholar]
  4. Harrison P. B., Jansson K., Kronenberg H., Mahony J. F., Tiller D. Cold agglutinin formation in patients undergoing haemodialysis. A possible relationship to dialyser re-use. Aust N Z J Med. 1975 Jun;5(3):195–197. doi: 10.1111/j.1445-5994.1975.tb04567.x. [DOI] [PubMed] [Google Scholar]
  5. Hart D. N., Fabre J. W. Kidney-specific allo- and autoantibodies in the alloantibody response to rat kidney: the use of kidney homogenate as a target for serological analysis. Clin Exp Immunol. 1980 Apr;40(1):111–119. [PMC free article] [PubMed] [Google Scholar]
  6. Iwaki Y., Terasaki P. I., Park M. S., Billing R. Enhancement of human kidney allografts by cold B-lymphocyte cytotoxins. Lancet. 1978 Jun 10;1(8076):1228–1229. doi: 10.1016/s0140-6736(78)92464-9. [DOI] [PubMed] [Google Scholar]
  7. Klassen J., Kano K., Milgrom F., Menno A. B., Anthone S., Anthone R., Sepulveda M., Elwood C. M., Andres G. A. Tubular lesions produced by autoantibodies to tubular basement membrane in human renal allografts. Int Arch Allergy Appl Immunol. 1973;45(5):675–689. doi: 10.1159/000231067. [DOI] [PubMed] [Google Scholar]
  8. Kreisler M. J., Hirata A. A., Terasaki P. I. Cytotoxins in disease. 3. Antibodies against lymphocytes produced by vaccination. Transplantation. 1970 Nov;10(5):411–415. [PubMed] [Google Scholar]
  9. MacLennan I. C. Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant Rev. 1972;13:67–90. doi: 10.1111/j.1600-065x.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  10. Milgrom F., Klassen J., Kano K. Autoimmunity and homograft rejection. J Reticuloendothel Soc. 1970 Feb;7(2):264–279. [PubMed] [Google Scholar]
  11. Naito S., Mickey M. R., Hirata A., Terasaki P. I. Autolymphocytotoxins following immunization by pregnancy, transplantation, and disease. Tissue Antigens. 1971;1(5):219–228. doi: 10.1111/j.1399-0039.1971.tb00099.x. [DOI] [PubMed] [Google Scholar]
  12. Ozturk G., Terasaki P. I. Non-HLA lymphocyte cytotoxins in various diseases. Tissue Antigens. 1979 Jul;14(1):52–58. doi: 10.1111/j.1399-0039.1979.tb00821.x. [DOI] [PubMed] [Google Scholar]
  13. Park M. S., Terasaki P. I., Bernoco D. Autoantibody against B lymphocytes. Lancet. 1977 Sep 3;2(8036):465–467. doi: 10.1016/s0140-6736(77)91598-7. [DOI] [PubMed] [Google Scholar]
  14. Paul L. C., Van Es L. A., Kalff M. W., Fleuren G. J. Antibodies directed against renal collecting ducts in sera of human renal allograft recipients. Clin Exp Immunol. 1979 Nov;38(2):235–242. [PMC free article] [PubMed] [Google Scholar]
  15. Reekers P., Lucassen-Hermans R., Koene R. A., Kunst V. A. Autolymphocytotoxic antibodies and kidney transplantation. Lancet. 1977 May 14;1(8020):1063–1064. doi: 10.1016/s0140-6736(77)91308-3. [DOI] [PubMed] [Google Scholar]
  16. Schmidt G. M., Bross K. J., Blume K. G., Beutler E. Demonstration of HLA antigens on human skin fibroblasts by the peroxidase-antiperoxidase method. Transplantation. 1980 Apr;29(4):344–347. doi: 10.1097/00007890-198004000-00019. [DOI] [PubMed] [Google Scholar]
  17. Stastny P., Austin C. L. Successful kidney trasnplant in patient with positive crossmatch due to autoantibodies. Transplantation. 1976 May;21(5):399–402. doi: 10.1097/00007890-197605000-00007. [DOI] [PubMed] [Google Scholar]
  18. Ting A., Morris P. J. Development of donor-specific B lymphocyte antibodies after renal transplantation. No correlation with graft outcome. Transplantation. 1979 Jul;28(1):13–17. doi: 10.1097/00007890-197907000-00004. [DOI] [PubMed] [Google Scholar]
  19. Ting A., Morris P. J. Reactivity of autolymphocytotoxic antibodies from dialysis patients with lymphocytes from chronic lymphocytic leukemia (CLL) patients. Transplantation. 1978 Jan;25(1):31–33. doi: 10.1097/00007890-197801000-00007. [DOI] [PubMed] [Google Scholar]
  20. Ting A., Morris P. J. Renal transplantation and B-cell cross-matches with autoantibodies and alloantibodies. Lancet. 1977 Nov 26;2(8048):1095–1097. doi: 10.1016/s0140-6736(77)90544-x. [DOI] [PubMed] [Google Scholar]
  21. Waldmann T. A., Blaese R. M., Broder S., Krakauer R. S. Disorders of suppressor immunoregulatory cells in the pathogenesis of immunodeficiency and autoimmunity. Ann Intern Med. 1978 Feb;88(2):226–238. doi: 10.7326/0003-4819-88-2-226. [DOI] [PubMed] [Google Scholar]
  22. Weigle W. O. Recent observations and concepts in immunological unresponsiveness and autoimmunity. Clin Exp Immunol. 1971 Oct;9(4):437–447. [PMC free article] [PubMed] [Google Scholar]
  23. Wernet P. Human Ia-type alloantigens: methods of detection, aspects of chemistry and biology, markers for disease states. Transplant Rev. 1976;30:271–298. doi: 10.1111/j.1600-065x.1976.tb00223.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES