Skip to main content
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1981 Nov;46(2):301–304.

IgE plasma cells in human jejunum demonstrated by immune electron microscopy

S Patterson, Pamela Roebuck, T A E Platts-Mills, Margot Shiner, D Kingston, Joy R Pearson
PMCID: PMC1536401  PMID: 7039884

Abstract

An immune electron microscopy technique has been employed to detect IgE plasma cells in histologically normal human jejunal biopsies. Anti-IgE-labelled plasma cells have been found in the lamina propria of three out of eight biopsies studied. Thus this report ends speculation regarding the presence of IgE plasma cells in human jejunum and supports other data indicating local IgE production in mucosal tissues.

Full text

PDF
301

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S., Ternynck T. Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971 Dec;8(12):1175–1179. doi: 10.1016/0019-2791(71)90395-8. [DOI] [PubMed] [Google Scholar]
  2. Brandtzaeg P., Baklien K. Inconclusive immunohistochemistry of human IgE in mucosal pathology. Lancet. 1976 Jun 12;1(7972):1297–1298. doi: 10.1016/s0140-6736(76)91767-0. [DOI] [PubMed] [Google Scholar]
  3. Brown W. R., Borthistle B. K., Chen S. T. Immunoglobulin E (IgE) and IgE-containing cells in human gastrointestinal fluids and tissues. Clin Exp Immunol. 1975 May;20(2):227–237. [PMC free article] [PubMed] [Google Scholar]
  4. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  5. Kilby A., Walker-Smith J. A., Wood C. B. Letter: Small intestinal mucosa in cow's milk allergy. Lancet. 1975 Mar 1;1(7905):531–531. doi: 10.1016/s0140-6736(75)92887-1. [DOI] [PubMed] [Google Scholar]
  6. Mayrhofer G., Bazin H., Gowans J. L. Nature of cells binding anti-IgE in rats immunized with Nippostrongylus brasiliensis: IgE synthesis in regional nodes and concentration in mucosal mast cells. Eur J Immunol. 1976 Aug;6(8):537–545. doi: 10.1002/eji.1830060803. [DOI] [PubMed] [Google Scholar]
  7. Platts-Mills T. A. Local production of IgG, IgA and IgE antibodies in grass pollen hay fever. J Immunol. 1979 Jun;122(6):2218–2225. [PubMed] [Google Scholar]
  8. Platts-Mills T. A., Snajdr M. J., Ishizaka K., Frankland A. W. Measurement of IgE antibody by an antigen-binding assay: correlation with PK activity and IgG and IgA antibodies to allergens. J Immunol. 1978 Apr;120(4):1201–1210. [PubMed] [Google Scholar]
  9. Rosekrans P. C., Meijer C. J., Cornelisse C. J., van der Wal A. M., Lindeman J. Use of morphometry and immunohistochemistry of small intestinal biopsy specimens in the diagnosis of food allergy. J Clin Pathol. 1980 Feb;33(2):125–130. doi: 10.1136/jcp.33.2.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Savilahti E. Immunoglobulin-containing cells in the intestinal mucosa and immunoglobulins in the intestinal juice in children. Clin Exp Immunol. 1972 Jul;11(3):415–425. [PMC free article] [PubMed] [Google Scholar]
  11. Shiner M., Ballard J., Smith M. E. The small-intestinal mucosa in cow's milk allergy. Lancet. 1975 Jan 18;1(7899):136–134. doi: 10.1016/s0140-6736(75)91431-2. [DOI] [PubMed] [Google Scholar]
  12. Skinner J. M., Whitehead R. The plasma cells in inflammatory disease of the colon: a quantitative study. J Clin Pathol. 1974 Aug;27(8):643–646. doi: 10.1136/jcp.27.8.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tada T., Ishizaka K. Distribution of gamma E-forming cells in lymphoid tissues of the human and monkey. J Immunol. 1970 Feb;104(2):377–387. [PubMed] [Google Scholar]
  14. Webb J. A., Dorling J. The use of peroxidase-labelled antiglobulin for ultrastructural localization of tissue antigens reacting with serum antibodies. J Immunol Methods. 1973 Jan;2(2):145–157. doi: 10.1016/0022-1759(73)90012-4. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES