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. 1982 Mar;47(3):725–734.

Generation of a monoclonal antibody against facteur thymique serique (FTS).

K Ohga, G S Incefy, C Y Wang, R A Good
PMCID: PMC1536426  PMID: 7044627

Abstract

A monoclonal antibody against one of the thymic hormones, facteur thymique serique (FTS), was generated by hybridization between mouse NS-1 myeloma cells and BALB/c splenocytes, the latter obtained from BALB/c mice immunized with synthetic FTS coupled to mouse IgG. Enzyme-linked immunosorbent assay (ELISA) was developed and employed to detect the hybridoma secreting specific antibody. The monoclonal antibody (MA-FTS) was highly specific for FTS and did not cross-react with other thymic hormones or other unrelated peptides. MA-FTS could recognize FTS (or FTS-like molecule) in human serum and could absorb completely the FTS-like activity from human serum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach J. F., Bach M. A., Charreire J., Dardenne M., Pleau J. M. The mode of action of thymic hormones. Ann N Y Acad Sci. 1979;332:23–32. doi: 10.1111/j.1749-6632.1979.tb47094.x. [DOI] [PubMed] [Google Scholar]
  2. Bach J., Bardenne M., Pleau J., Rosa J. Biochemical characterisation of a serum thymic factor. Nature. 1977 Mar 3;266(5597):55–57. doi: 10.1038/266055a0. [DOI] [PubMed] [Google Scholar]
  3. Burton P., Iden S., Mitchell K., White A. Thymic hormone-like restoration by human prealbumin of azathioprine sensitivity of spleen cells from thymectomized mice. Proc Natl Acad Sci U S A. 1978 Feb;75(2):823–827. doi: 10.1073/pnas.75.2.823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dardenne M., Bach J. F. Demonstration and characterization of a serum factor produced by activated T cells. Immunology. 1977 Nov;33(5):643–651. [PMC free article] [PubMed] [Google Scholar]
  5. Dardenne M., Pleau J. M., Man N. K., Bach J. F. Structural study of circulating thymic factor: a peptide isolated from pig serum. I. Isolation and purification. J Biol Chem. 1977 Nov 25;252(22):8040–8044. [PubMed] [Google Scholar]
  6. Dardenne M., Pléau J. M., Bach J. F. Evidence of the presence in normal serum of a carrier of the serum thymic factor (FTS). Eur J Immunol. 1980 Feb;10(2):83–86. doi: 10.1002/eji.1830100203. [DOI] [PubMed] [Google Scholar]
  7. Dardenne M., Pléau J. M., Blouquit J. Y., Bach J. F. Characterization of facteur thymique sérique (FTS) in the thymus. II. Direct demonstration of the presence of FTS in thymosin fraction V. Clin Exp Immunol. 1980 Dec;42(3):477–482. [PMC free article] [PubMed] [Google Scholar]
  8. Goldstein A. L., Low T. L., McAdoo M., McClure J., Thurman G. B., Rossio J., Lai C. Y., Chang D., Wang S. S., Harvey C. Thymosin alpha1: isolation and sequence analysis of an immunologically active thymic polypeptide. Proc Natl Acad Sci U S A. 1977 Feb;74(2):725–729. doi: 10.1073/pnas.74.2.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goldstein G. Isolation of bovine thymin: a polypeptide hormone of the thymus. Nature. 1974 Jan 4;247(5435):11–14. doi: 10.1038/247011a0. [DOI] [PubMed] [Google Scholar]
  10. Incefy G. S., Dardenne M., Pahwa S., Grimes E., Pahwa R. N., Smithwick E., O'Reilly R., Good R. A. Thymic activity in severe combined immunodeficiency diseases. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1250–1253. doi: 10.1073/pnas.74.3.1250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Iwata T., Incefy G. S., Cunningham-Rundles S., Cunningham-Rundles C., Smithwick E., Geller N., O'Reilly R., Good R. A. Circulating thymic hormone activity in patients with primary and secondary immunodeficiency diseases. Am J Med. 1981 Sep;71(3):385–394. doi: 10.1016/0002-9343(81)90164-9. [DOI] [PubMed] [Google Scholar]
  12. Iwata T., Incefy G. S., Good R. A. Interaction between thymopoietin and facteur thymique serique in the rosette inhibition assay. Biochem Biophys Res Commun. 1979 Jun 27;88(4):1419–1424. doi: 10.1016/0006-291x(79)91138-0. [DOI] [PubMed] [Google Scholar]
  13. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  14. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  15. Low T. L., Thurman G. B., Chincarini C., McClure J. E., Marshall G. D., Hu S. K., Goldstein A. L. Current status of thymosin research: evidence for the existence of a family of thymic factors that control T-cell maturation. Ann N Y Acad Sci. 1979;332:33–48. doi: 10.1111/j.1749-6632.1979.tb47095.x. [DOI] [PubMed] [Google Scholar]
  16. Monier J. C., Dardenne M., Pléau J. M., Schmitt D., Deschaux P., Bach J. F. Characterization of facteur thymique sérique (FTS) in the thymus. I. Fixation of anti-FTS antibodies on thymic reticulo-epithelial cells. Clin Exp Immunol. 1980 Dec;42(3):470–476. [PMC free article] [PubMed] [Google Scholar]
  17. Safai B., Dardenne M., Incefy G. S., Bach J. F., Good R. A. Circulating thymic factor, facteur thymique serique (FTS), in mycosis fungoides and Sezary syndrome. Clin Immunol Immunopathol. 1979 Aug;13(4):402–412. doi: 10.1016/0090-1229(79)90081-3. [DOI] [PubMed] [Google Scholar]
  18. Twomey J. J., Goldstein G., Lewis V. M., Bealmear P. M., Good R. A. Bioassay determinations of thymopoietin and thymic hormone levels in human plasma. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2541–2545. doi: 10.1073/pnas.74.6.2541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Van Weemen B. K., Schuurs A. H.W.M. Immunoassay using antigen-enzyme conjugates. FEBS Lett. 1971 Jun 24;15(3):232–236. doi: 10.1016/0014-5793(71)80319-8. [DOI] [PubMed] [Google Scholar]
  20. Voller A., Bidwell D. E. A simple method for detecting antibodies to rubella. Br J Exp Pathol. 1975 Aug;56(4):338–339. [PMC free article] [PubMed] [Google Scholar]

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