Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1982 May;48(2):505–512.

Immunological reactions to liver-specific membrane lipoprotein (LSP) in experimental autoimmune liver disease in rabbits.

R M Uibo, H J Helin, K J Krohn
PMCID: PMC1536450  PMID: 7105494

Abstract

Humoral and cellular immunity to a liver-specific membrane lipoprotein (LSP) was followed in rabbits immunized either with allogenic rabbit LSP (RLSP) or with xenogenic human (HLSP) or bovine (BLSP) antigens. After 4 months of immunization, all rabbits showed histological changes in liver resembling those found in chronic aggressive hepatitis and occasionally also those seen in primary biliary cirrhosis in man. Antibodies reacting to both allogenic as well as xenogenic LSP preparations were found with an enzyme-linked solid phase immunosorbent assay (ELISA) in all immunized rabbits. Lymphocyte transformation tests in unimmunized rabbits showed an occasional response to some of the LSP preparations and a strong response towards an equivalent rabbit kidney preparation (RKSP). After immunization, reactions to LSP preparations increased significantly, while no response to RKSP was observed. The results confirm previous findings and further demonstrate that similar LSP containing preparations from human, bovine and rabbit sources share autoantigenic, liver-specific determinants. In addition, species specific determinants seem to be present in each of the LSP preparations studied.

Full text

PDF
505

Images in this article

510Fig. 4
on p.510

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Behrens U. J., Paronetto F. Studies on "liver-specific" antigens. I. Evaluation of the liver specificity of "LSP" and "LP-2". Gastroenterology. 1979 Nov;77(5):1045–1052. [PubMed] [Google Scholar]
  2. Cochrane A. M., Moussouros A., Thomsom A. D., Eddleston A. L., Wiiliams R. Antibody-dependent cell-mediated (K cell) cytotoxicity against isolated hepatocytes in chronic active hepatitis. Lancet. 1976 Feb 28;1(7957):441–444. doi: 10.1016/s0140-6736(76)91472-0. [DOI] [PubMed] [Google Scholar]
  3. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  4. Feighery C., McDonald G. S., Greally J. F., Weir D. G. Histological and immunological investigation of liver-specific protein (LSP) immunized rabbits compared with patients with liver disease. Clin Exp Immunol. 1981 Jul;45(1):143–151. [PMC free article] [PubMed] [Google Scholar]
  5. Galbraith R. M., Fudenberg H. H. Autoimmunity in chronic active hepatitis and diabetes mellitus. Clin Immunol Immunopathol. 1977 Jul;8(1):116–149. doi: 10.1016/0090-1229(77)90099-x. [DOI] [PubMed] [Google Scholar]
  6. Jensen D. M., McFarlane I. G., Portmann B. S., Eddleston A. L., Williams R. Detection of antibodies directed against a liver-specific membrane lipoprotein in patients with acute and chronic active hepatitis. N Engl J Med. 1978 Jul 6;299(1):1–7. doi: 10.1056/NEJM197807062990101. [DOI] [PubMed] [Google Scholar]
  7. Kakumu S., Arakawa Y., Goji H., Kashio T., Yata K. Occurrence and significance of antibody to liver-specific membrane lipoprotein by double-antibody immunoprecipitation method in sera of patients with acute and chronic liver diseases. Gastroenterology. 1979 Apr;76(4):665–672. [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Manns M., Meyer zum Büschenfelde K. H., Hütteroth T., Hopf U. The liver specific protein: evidence for species-specific and non-species-specific determinants. J Clin Lab Immunol. 1980 Jan;3(1):9–13. [PubMed] [Google Scholar]
  10. McFarlane I. G., Wojcicka B. M., Zucker G. M., Eddleston A. L., Williams R. Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol. 1977 Mar;27(3):381–390. [PMC free article] [PubMed] [Google Scholar]
  11. Meyer zum Büschenfelde K. H., Hopf U. Studies on the pathogenesis of experimental chronic active hepatitis in rabbits. I. Induction of the disease and protective effect of allogeneic liver specific proteins. Br J Exp Pathol. 1974 Oct;55(5):498–508. [PMC free article] [PubMed] [Google Scholar]
  12. Thomson A. D., Cochrane M. A., McFarlane I. G., Eddleston A. L., Williams R. Lymphocyte cytotoxicity to isolated hepatocytes in chronic active hepatitis. Nature. 1974 Dec 20;252(5485):721–722. doi: 10.1038/252721a0. [DOI] [PubMed] [Google Scholar]
  13. Uotila A., Marnela K. M., Hamblin A., Krohn K. Human transfer factor in vitro: identification of L-serine and glycine as components with augmenting effect in lymphocyte transformation assay. Scand J Immunol. 1978;7(6):495–502. doi: 10.1111/j.1365-3083.1978.tb00483.x. [DOI] [PubMed] [Google Scholar]
  14. Wands J. R., Perrotto J. L., Alpert E., Isselbacher K. J. Cell-mediated immunity in acute and chronic hepatitis. J Clin Invest. 1975 May;55(5):921–929. doi: 10.1172/JCI108021. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES