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. 1982 May;48(2):289–299.

Trypanosoma theileri: antibody-dependent killing by purified populations of bovine leucocytes.

J Townsend, W P Duffus
PMCID: PMC1536476  PMID: 7105486

Abstract

Bovine leucocytes were assayed for their cytotoxic activity against Trypanosoma theileri, a large haemoflagellate parasite of cattle. Cytotoxicity was assessed by 3H-uridine release from pre-labelled parasites and also by light microscopy. Cytotoxicity proved to be totally dependent upon the presence of specific antibody. Serum and the immunoglobulin fraction of colostrum from normal adult cattle and serum from normal colostrum fed calves mediated cytotoxicity; serum from SPF colostrum deprived calves possessed no cytotoxic activity. Neutrophils, eosinophils, monocytes and macrophages obtained from both the peripheral blood and mammary gland of heifers were cytotoxic to T. theileri epimastigotes. Lymphocytes failed to mediate cytotoxicity either in the presence or absence of specific antibody. Despite the large size of this trypanosome all effector cells phagocytosed T. theileri. Phagocytosis by macrophages and eosinophils preceded specific isotope release, however neutrophils mediated 50% specific isotope release during the phagocytic period, suggesting extracellular cell-mediated lysis.

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Selected References

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  1. Abrahamsohn I. A., Silva W. D. Antibody dependent cell-mediated cytotoxicity against Trypanosoma cruzi. Parasitology. 1977 Dec;75(3):317–323. doi: 10.1017/s0031182000051866. [DOI] [PubMed] [Google Scholar]
  2. Alcantara A., Brener Z. The in vitro interaction of Trypanosoma cruzi bloodstream forms and mouse peritoneal macrophages. Acta Trop. 1978 Sep;35(3):209–219. [PubMed] [Google Scholar]
  3. Alcantara A., Brener Z. Trypanosoma cruzi: role of macrophage membrane components in the phagocytosis of bloodstream forms. Exp Parasitol. 1980 Aug;50(1):1–6. doi: 10.1016/0014-4894(80)90002-8. [DOI] [PubMed] [Google Scholar]
  4. Babiuk L. A., Rouse B. T. Immune interferon production by lymphoid cells: role in the inhibition of herpesviruses. Infect Immun. 1976 Jun;13(6):1567–1578. doi: 10.1128/iai.13.6.1567-1578.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Baker J. R., Green S. M., Chaloner L. A., Gaborak M. Trypanosoma (Schizotrypanum) dionisii of pipistrellus pipistrellus (chiroptera): intra- and extracellular development in vitro. Parasitology. 1972 Oct;65(2):251–263. doi: 10.1017/s0031182000045030. [DOI] [PubMed] [Google Scholar]
  6. Cardoni R. L., Olabuenaga S. E., Riera N. E., de Bracco M. M. Mecanismos inmunológicos efectores en la infección con Trypanosoma cruzi. Actividad citotóxica dependiente de anticuerpo de los leucocitos polimorfonucleares humanos. Medicina (B Aires) 1980;40 (Suppl 1):77–84. [PubMed] [Google Scholar]
  7. Carlson G. P., Kaneko J. J. Isolation of leukocytes from bovine peripheral blood. Proc Soc Exp Biol Med. 1973 Mar;142(3):853–856. doi: 10.3181/00379727-142-37131. [DOI] [PubMed] [Google Scholar]
  8. Cook R. M. Attachment of Trypanosoma brucei to rabbit peritoneal exudate cells. Int J Parasitol. 1981 Apr;11(2):149–156. doi: 10.1016/0020-7519(81)90078-3. [DOI] [PubMed] [Google Scholar]
  9. Duffus W. P., Franks D. In vitro effect of immune serum and bovine granulocytes on juvenile Fasciola hepatica. Clin Exp Immunol. 1980 Sep;41(3):430–440. [PMC free article] [PubMed] [Google Scholar]
  10. Ferrante A., Jenkin C. R. The role of the macrophage in immunity to Trypanosoma lewisi infections in the rat. Cell Immunol. 1979 Feb;42(2):327–335. doi: 10.1016/0008-8749(79)90198-9. [DOI] [PubMed] [Google Scholar]
  11. GOBLE F. C., BOYD J. L. Reticulo-endothelial blockade in experimental Chagas' disease. J Parasitol. 1962 Apr;48:223–228. [PubMed] [Google Scholar]
  12. Grewal A. S., Babiuk L. A. Induction, isolation and surface marker studies on bovine eosinophils. J Immunol Methods. 1979;25(1):65–75. doi: 10.1016/0022-1759(79)90167-4. [DOI] [PubMed] [Google Scholar]
  13. Grewal A. S., Rouse B. T., Babiuk L. A. Mechanisms of resistant of herpesviruses: comparison of the effectiveness of different cell types in mediating antibody-dependent cell-mediated cytotoxicity. Infect Immun. 1977 Mar;15(3):698–703. doi: 10.1128/iai.15.3.698-703.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Grewal A. S., Rouse B. T. Characterization of bovine leukocytes involved in antibody-dependent cell cytotoxicity. Int Arch Allergy Appl Immunol. 1979;60(2):169–177. doi: 10.1159/000232339. [DOI] [PubMed] [Google Scholar]
  15. Guidry A. J., Paape M. J., Pearson R. E., Williams W. F. Effect of local immunization of the mammary gland on phagocytosis and intracellular kill of Staphylococcus aureus by polymorphonuclear neutrophils. Am J Vet Res. 1980 Sep;41(9):1427–1431. [PubMed] [Google Scholar]
  16. Kierszenbaum F. Antibody-dependent killing of bloodstream forms of Trypanosoma cruzi by human peripheral blood leukocytes. Am J Trop Med Hyg. 1979 Nov;28(6):965–968. doi: 10.4269/ajtmh.1979.28.965. [DOI] [PubMed] [Google Scholar]
  17. Kierszenbaum F., Knecht E., Budzko D. B., Pizzimenti M. C. Phagocytosis: a defense mechanism against infection with Trypanosoma cruzi. J Immunol. 1974 May;112(5):1839–1844. [PubMed] [Google Scholar]
  18. Kraft D. Characteristics and clinical importance of killer (K)-cells. Br J Dermatol. 1976 Oct;95(4):449–452. doi: 10.1111/j.1365-2133.1976.tb00850.x. [DOI] [PubMed] [Google Scholar]
  19. Kress Y., Tanowitz H., Bloom B., Wittner M. Trypanosoma cruzi: infection of normal and activated mouse macrophages. Exp Parasitol. 1977 Apr;41(2):385–396. doi: 10.1016/0014-4894(77)90110-2. [DOI] [PubMed] [Google Scholar]
  20. Kuhn R. E., Vaughn R. T. An immunocytotoxic assay for Trypanosoma cruzi. Int J Parasitol. 1976 Apr;6(2):129–134. doi: 10.1016/0020-7519(76)90069-2. [DOI] [PubMed] [Google Scholar]
  21. Kuhn R. E., Vaughn R. T., Iannuzzi N. P. The in vivo distribution of 51Cr-labeled Trypanosoma cruzi in mice. Int J Parasitol. 1974 Dec;4(6):585–588. doi: 10.1016/0020-7519(74)90022-8. [DOI] [PubMed] [Google Scholar]
  22. Kuntz M. M., Innes J. B., Weksler M. E. Lymphocyte transformation induced by autologous cells. IV. Human T-lymphocyte proliferation induced by autologous or allogeneic non-T lymphocytes. J Exp Med. 1976 May 1;143(5):1042–1054. doi: 10.1084/jem.143.5.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. LANGE D. E., LYSENKO M. G. In vitro phagocytosis of Trypanosoma lewisi by rat exudative cells. Exp Parasitol. 1960 Sep;10:39–42. doi: 10.1016/0014-4894(60)90081-3. [DOI] [PubMed] [Google Scholar]
  24. López A. F., Moreno M. M., Sanderson C. J. The lysis of Trypanosoma cruzi epimastigotes by eosinophils and neutrophils. Int J Parasitol. 1978 Dec;8(6):485–489. doi: 10.1016/0020-7519(78)90069-3. [DOI] [PubMed] [Google Scholar]
  25. Mach J. P., Pahud J. J. Secretory IgA, a major immunoglobulin in most bovine external secretions. J Immunol. 1971 Feb;106(2):552–563. [PubMed] [Google Scholar]
  26. Madeira E. D., de Andrade A. F., Bunn-Moreno M. M., Barcinski M. Antibody-dependent cellular cytotoxicity of Trypanosoma cruzi: characterization of the effector cell from normal human blood. Infect Immun. 1979 Jul;25(1):34–38. doi: 10.1128/iai.25.1.34-38.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Olabuenaga S. E., Cardoni R. L., Segura E. L., Riera N. E., de Bracco M. M. Antibody-dependent cytolysis of Trypanosoma cruzi by human polymorphonuclear leukocytes. Cell Immunol. 1979 Jun;45(1):85–93. doi: 10.1016/0008-8749(79)90364-2. [DOI] [PubMed] [Google Scholar]
  28. Patton C. L. Trypanosoma lewisi: influence of sera and peritoneal exudate cells. Exp Parasitol. 1972 Jun;31(3):370–377. doi: 10.1016/0014-4894(72)90099-9. [DOI] [PubMed] [Google Scholar]
  29. Rimoldi M. T., Cardoni R. L., Olabuenaga S. E., de Bracco M. M. Trypanosoma cruzi: sequence of phagocytosis and cytotoxicity by human polymorphonuclear leucocytes. Immunology. 1981 Apr;42(4):521–527. [PMC free article] [PubMed] [Google Scholar]
  30. Rouse B. T., Babiuk L. A. Host responses to infectious bovine rhinotracheitis virus. III. Isolation and immunologic activities of bovine T lymphocytes. J Immunol. 1974 Nov;113(5):1391–1398. [PubMed] [Google Scholar]
  31. Sanderson C. J., Lopez A. F., Moreno M. M. Eosinophils and not lymphoid K cells kill Trypanosoma cruzi epimastigotes. Nature. 1977 Jul 28;268(5618):340–341. doi: 10.1038/268340a0. [DOI] [PubMed] [Google Scholar]
  32. Sanderson C. J., Moreno M. M., Lopez A. F. Antibody dependent cell-mediated cytotoxicity of Trypanosoma cruzi: the release of tritium-labelled RNA, DNA and protein. Parasitology. 1978 Jun;76(3):299–307. doi: 10.1017/s0031182000048174. [DOI] [PubMed] [Google Scholar]
  33. Sanderson C. J., de Souza W. A morphological study of the interaction between Trypanosoma cruzi and rat eosinophils, neutrophils and macrophages in vitro. J Cell Sci. 1979 Jun;37:275–286. doi: 10.1242/jcs.37.1.275. [DOI] [PubMed] [Google Scholar]
  34. Stevens D. R., Moulton J. E. Ultrastructural and immunological aspects of the phagocytosis of Trypanosoma brucei by mouse peritoneal macrophages. Infect Immun. 1978 Mar;19(3):972–982. doi: 10.1128/iai.19.3.972-982.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Takayanagi T., Nakatake Y., Enriquez G. L. Attachment and ingestion of Trypanosoma gambiense to the rat macrophage by specific antiserum. J Parasitol. 1974 Apr;60(2):336–339. [PubMed] [Google Scholar]
  36. Takayanagi T., Nakatake Y. Trypanosoma gambiense: the binding activity of antiserum to macrophages. Exp Parasitol. 1977 Jun;42(1):21–26. doi: 10.1016/0014-4894(77)90057-1. [DOI] [PubMed] [Google Scholar]
  37. Thorne K. J., Glauert A. M., Svvennsen R. J., Franks D. Phagocytosis and killing of Trypanosoma dionisii by human neutrophils, eosinophils and monocytes. Parasitology. 1979 Dec;79(3):367–379. doi: 10.1017/s0031182000053762. [DOI] [PubMed] [Google Scholar]
  38. Tizard I. R., Soltys M. A. Macrophage cytophilic antibodies to Trypanosoma brucei in rabbits. Trans R Soc Trop Med Hyg. 1971;65(3):407–408. doi: 10.1016/0035-9203(71)90029-0. [DOI] [PubMed] [Google Scholar]
  39. Vasquez R., Cattan A., Herbert W. J. Proceedings: Macrophage-cytophilic antibodies in trypanosome infections in mice. Trans R Soc Trop Med Hyg. 1975;69(4):436–436. [PubMed] [Google Scholar]
  40. Wardley R. C., Rouse B. T., Babiuk L. A. Antibody dependent cytotoxicity mediated by neutrophils: a possible mechanism of antiviral defense. J Reticuloendothel Soc. 1976 May;19(5):323–332. [PubMed] [Google Scholar]
  41. Williams D. M., Remington J. S. Effect of human monocytes and macrophages on Trypanosoma cruzi. Immunology. 1977 Jan;32(1):19–23. [PMC free article] [PubMed] [Google Scholar]

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