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. 1982 May;48(2):307–314.

An adoptive cell transfer system for the evaluation of immunity to Herpes simplex virus in mice.

L Favila, E L Howes, W A Taylor, N A Mitchison
PMCID: PMC1536478  PMID: 7105488

Abstract

Lymphocytes adoptively transferred from syngeneic immune donors protect mice against challenge with Herpes simplex virus type 1. Normal mice require transfer 3 x 10(7) spleen cells for protection. Sublethal irradiation (450 rad) decreases the number required five-fold. Lymphocytes from non-immune donors do not protect, and hyperimmunization does not enhance the protection efficiency of donors. The viral LD50 varies through a range 10(5)-fold during the period of recovery from this amount of radiation, but over the same period there is little variation in the number of cells required for protection. Nor is there much variation in this number between strains of mice naturally susceptible (CBA) and resistant (C57) to the virus. We conclude that natural resistance operates at a level of virus handling prior to operation of the lymphocyte system, perhaps at the generation of interferon. As few as 1.3 x 10(6) immune T lymphocytes can protect against challenge provided that they are transferred together with normal spleen cells. We conclude that primed lymphocytes act in co-operation with non-immune cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chun M., Fernandes G., Hoffmann M. K. Mechanism of NK cell activation: relationship between Qa5+ NK cells and lymphocytes. J Immunol. 1981 Jan;126(1):331–334. [PubMed] [Google Scholar]
  2. Ennis F. A. Host defense mechanisms against herpes simplex virus. II. Protection conferred by sensitized spleen cells. J Infect Dis. 1973 Jun;127(6):632–638. doi: 10.1093/infdis/127.6.632. [DOI] [PubMed] [Google Scholar]
  3. Hirsch M. S., Zisman B., Allison A. C. Macrophages and age-dependent resistance to Herpes simplex virus in mice. J Immunol. 1970 May;104(5):1160–1165. [PubMed] [Google Scholar]
  4. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  5. Lopez C. Genetics of natural resistance to herpesvirus infections in mice. Nature. 1975 Nov 13;258(5531):152–153. doi: 10.1038/258152a0. [DOI] [PubMed] [Google Scholar]
  6. Lopez C., Ryshke R., Bennett M. Marrow-dependent cells depleted by 89Sr mediate genetic resistance to herpes simplex virus type 1 infection in mice. Infect Immun. 1980 Jun;28(3):1028–1032. doi: 10.1128/iai.28.3.1028-1032.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McDonald V., Phillips R. S. Plasmodium chabaudi in mice. Adoptive transfer of immunity with enriched populations of spleen T and B lymphocytes. Immunology. 1978 May;34(5):821–830. [PMC free article] [PubMed] [Google Scholar]
  8. Nash A. A., Phelan J., Wildy P. Cell-mediated immunity in herpes simplex virus-infected mice: H-2 mapping of the delayed-type hypersensitivity response and the antiviral T cell response. J Immunol. 1981 Apr;126(4):1260–1262. [PubMed] [Google Scholar]
  9. Oakes J. E. Role for cell-mediated immunity in the resistance of mice to subcutaneous herpes simplex virus infection. Infect Immun. 1975 Jul;12(1):166–172. doi: 10.1128/iai.12.1.166-172.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Piontek G. E., Weltzin R., Tompkins W. A. Enhanced cytotoxicity of mouse natural killer cells for vaccinia and herpes virus-infected targets. J Reticuloendothel Soc. 1980 Feb;27(2):175–188. [PubMed] [Google Scholar]
  11. Rager-Zisman B., Allison A. C. Mechanism of immunologic resistance to herpes simplex virus 1 (HSV-1) infection. J Immunol. 1976 Jan;116(1):35–40. [PubMed] [Google Scholar]
  12. Zawatzky R., Hilfenhaus J., Marcucci F., Kirchner H. Experimental infection of inbred mice with herpes simplex virus type 1. I. Investigation of humoral and cellular immunity and of interferon induction. J Gen Virol. 1981 Mar;53(Pt 1):31–38. doi: 10.1099/0022-1317-53-1-31. [DOI] [PubMed] [Google Scholar]
  13. Zinkernagel R. M., Althage A., Adler B., Blanden R. V., Davidson W. F., Kees U., Dunlop M. B., Shreffler D. C. H-2 restriction of cell-mediated immunity to an intracellular bacterium: effector T cells are specific for Listeria antigen in association with H-21 region-coded self-markers. J Exp Med. 1977 May 1;145(5):1353–1367. doi: 10.1084/jem.145.5.1353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zinkernagel R. M., Welsh R. M. H-2 compatibility requirement for virus-specific T cell-mediated effector functions in vivo. I. Specificity of T cells conferring antiviral protection against lymphocytic choriomeningitis virus is associated with H-2K and H-2D. J Immunol. 1976 Nov;117(5 Pt 1):1495–1502. [PubMed] [Google Scholar]

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