Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1982 Aug;49(2):355–363.

Cytoplasmic as opposed to surface Ia antigens expressed on human peripheral blood lymphocytes and monocytes.

F M Hofman, R J Billing, J W Parker, C R Taylor
PMCID: PMC1536486  PMID: 6181924

Abstract

Peripheral blood leucocytes were examined for the presence of HLA-Dr or Ia-like antigen on the cell surface and in the cytoplasm. Surface staining of viable cell suspensions, using monoclonal anti-Ia antibody in both immunofluorescence and immunoperoxidase methods gave similar results. Using a newly developed immunoperoxidase double staining procedure, which stains the cells when either viable or fixed, both the surface and cytoplasm of the individual cells were labelled and examined. Twenty to thirty percent of the mononuclear cells were positive for Ia staining on the cell surface and in the cytoplasm. Morphologically these positive cells were identified as both lymphocytes and monocytes. A small percentage (5-9%) of these monocytes and lymphocytes consistently labelled only cytoplasmic Ia determinants. In contrast, only surface binding was observed with monoclonal antibodies recognizing T cell subpopulations. The ability to detect intracellular Ia antigenic determinants is of value in distinguishing stages of lymphocyte and monocyte development.

Full text

PDF
355

Images in this article

358Fig. 2
on p.358

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beller D. I., Kiely J. M., Unanue E. R. Regulation of macrophage populations. I. Preferential induction of Ia-rich peritoneal exudates by immunologic stimuli. J Immunol. 1980 Mar;124(3):1426–1432. [PubMed] [Google Scholar]
  2. Beller D. I., Unanue E. R. Regulation of macrophage populations. II. Synthesis and expression of Ia antigens by peritoneal exudate macrophages is a transient event. J Immunol. 1981 Jan;126(1):263–269. [PubMed] [Google Scholar]
  3. Belzer M. B., Fitchen J. H., Ferrone S., Foon K. A., Billing R. J., Golde D. W. Expression of Ia-like antigens in human erythroid progenitor cells as determined by monoclonal antibodies and heteroantiserum to Ia-like antigens. Clin Immunol Immunopathol. 1981 Jul;20(1):111–115. doi: 10.1016/0090-1229(81)90167-7. [DOI] [PubMed] [Google Scholar]
  4. Bhan A. K., Reinherz E. L., Poppema S., McCluskey R. T., Schlossman S. F. Location of T cell and major histocompatibility complex antigens in the human thymus. J Exp Med. 1980 Oct 1;152(4):771–782. doi: 10.1084/jem.152.4.771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Billing R., Rafizadeh B., Drew I., Hartman G., Gale R., Terasaki P. Human B-lymphocyte antigens expressed by lymphocytic and myelocytic leukemia cells. I. Detection by rabbit antisera. J Exp Med. 1976 Jul 1;144(1):167–178. doi: 10.1084/jem.144.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Billing R., Safani M., Lesch J. Two-chain subunit structure of HLA-D antigens. Tissue Antigens. 1977 Oct;10(4):267–272. doi: 10.1111/j.1399-0039.1977.tb00756.x. [DOI] [PubMed] [Google Scholar]
  7. Cline M. J., Billing R. Antigens expressed by human B lymphocytes and myeloid stem cells. J Exp Med. 1977 Oct 1;146(4):1143–1145. doi: 10.1084/jem.146.4.1143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. De Cock W., De Cree J., Verhaegen H. An enzyme immunoassay for the enumeration of peripheral human T-lymphocytes with OKT3.PAN monoclonal antibody. J Immunol Methods. 1981;43(2):131–134. doi: 10.1016/0022-1759(81)90016-8. [DOI] [PubMed] [Google Scholar]
  9. Emerson S. G., Murphy D. B., Cone R. E. Ia antigen turnover. II. The kinetics of biosynthesis and release of Ia alpha- and beta-chains by murine spleen cells in culture. J Immunol. 1980 Jul;125(1):406–410. [PubMed] [Google Scholar]
  10. Greaves M. F., Verbi W., Festenstein H., Papasteriadis C., Jaraquemada D., Hayward A. "Ia-like" antigens on human T cells. Eur J Immunol. 1979 May;9(5):356–362. doi: 10.1002/eji.1830090504. [DOI] [PubMed] [Google Scholar]
  11. Hart D. N., Fabre J. W. Endogenously produced Ia antigens within cells of convoluted tubules of rat kidney. J Immunol. 1981 Jun;126(6):2109–2113. [PubMed] [Google Scholar]
  12. Hocking W., Billing R., Foon K., Golde D. Human alveolar macrophages express Ia-like antigens. Blood. 1981 Nov;58(5):1040–1042. [PubMed] [Google Scholar]
  13. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  14. Hämmerling G. J. Tissue distribution of Ia antigens and their expression on lymphocyte subpopulations. Transplant Rev. 1976;30:64–82. [PubMed] [Google Scholar]
  15. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  17. Lloyd K. O., Ng J., Dippold W. G. Analysis of the biosynthesis of HLA-DR glycoproteins in human malignant melanoma cell lines. J Immunol. 1981 Jun;126(6):2408–2413. [PubMed] [Google Scholar]
  18. Lu C. Y., Beller D. I., Unanue E. R. During ontogeny, Ia-bearing accessory cells are found early in the thymus but late in the spleen. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1597–1601. doi: 10.1073/pnas.77.3.1597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Natali P. G., De Martino C., Quaranta V., Nicotra M. R., Frezza F., Pellegrino M. A., Ferrone S. Expression of Ia-like antigens in normal human nonlymphoid tissues. Transplantation. 1981 Jan;31(1):75–78. doi: 10.1097/00007890-198101000-00017. [DOI] [PubMed] [Google Scholar]
  20. Niederhuber J. E., Frelinger J. A. Expression of Ia antigens on T and B cells and their relationship to immune-response functions. Transplant Rev. 1976;30:101–121. [PubMed] [Google Scholar]
  21. Owen J. J., Cooper M. D., Raff M. C. In vitro generation of B lymphocytes in mouse foetal liver, a mammalian 'bursa equivalent'. Nature. 1974 May 24;249(455):361–363. doi: 10.1038/249361a0. [DOI] [PubMed] [Google Scholar]
  22. Puri J., Lonai P. Mechanism of antigen binding by T cells. H-2(I-A)-restricted binding of antigen plus Ia by helper cells. Eur J Immunol. 1980 Apr;10(4):273–281. doi: 10.1002/eji.1830100410. [DOI] [PubMed] [Google Scholar]
  23. Schlossman S. F., Chess L., Humphreys R. E., Strominger J. L. Distribution of Ia-like molecules on the surface of normal and leukemic human cells. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1288–1292. doi: 10.1073/pnas.73.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Seymour G. J., Greaves M. F., Janossy G. Identification of cells expressing T and p28,33 (Ia-like) antigens in sections of human lymphoid tissue. Clin Exp Immunol. 1980 Jan;39(1):66–75. [PMC free article] [PubMed] [Google Scholar]
  25. Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
  26. Springer T. A., Kaufman J. F., Terhorst C., Strominger J. L. Purification and structural characterisation of human HLA-linked B-cell antigens. Nature. 1977 Jul 21;268(5617):213–218. doi: 10.1038/268213a0. [DOI] [PubMed] [Google Scholar]
  27. Taylor C. R. Immunoperoxidase techniques: practical and theoretical aspects. Arch Pathol Lab Med. 1978 Mar;102(3):113–121. [PubMed] [Google Scholar]
  28. Warnke R., Levy R. Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method. J Histochem Cytochem. 1980 Aug;28(8):771–776. doi: 10.1177/28.8.7003003. [DOI] [PubMed] [Google Scholar]
  29. Wekerle H., Ketelsen U. P. Thymic nurse cells--Ia-bearing epithelium involved in T-lymphocyte differentiation? Nature. 1980 Jan 24;283(5745):402–404. doi: 10.1038/283402a0. [DOI] [PubMed] [Google Scholar]
  30. Winchester R. J., Ross G. D., Jarowski C. I., Wang C. Y., Halper J., Broxmeyer H. E. Expression of Ia-like antigen molecules on human granulocytes during early phases of differentiation. Proc Natl Acad Sci U S A. 1977 Sep;74(9):4012–4016. doi: 10.1073/pnas.74.9.4012. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES