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. 1982 Aug;49(2):410–418.

Disorders of regulatory T cells in patients with selective IgA deficiency and its relationship to associated autoimmune phenomena.

E G De la Concha, J L Subiza, G Fontán, D Pascual-Salcedo, J Sequí, A Bootello
PMCID: PMC1536498  PMID: 6215200

Abstract

In vitro lymphocyte function of 13 patients with selective IgA deficiency was studied. IgG, IgA and IgM secretion by pokeweed mitogen-stimulated peripheral blood lymphocytes from normal donors and IgA deficient patients was measured by an enzyme-linked immunosorbent assay. Addition of concanavalin A or hydrocortisone and co-cultures of B and T cell enriched populations from patients and normal donors, allowed us to investigate B cell Ig production and T cell regulatory abnormalities. IgA production by these patients' B cells was either absent or very low, as compared to their total Ig production, even in the presence of optimal T cell help. Several T cell immunoregulatory abnormalities were seen in different patients. A moderate increase in suppressor activity selective for IgA production was observed in some, but does not appear to play a major role in the pathogenesis of the IgA deficiency. In others, with associated autoimmune phenomena, a decrease in suppressor T cell function was found.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atwater J. S., Tomasi T. B., Jr Suppressor cells and IgA deficiency. Clin Immunol Immunopathol. 1978 Mar;9(3):379–384. doi: 10.1016/0090-1229(78)90110-1. [DOI] [PubMed] [Google Scholar]
  2. Cassidy J. T., Oldham G., Platts-Mills T. A. Functional assessment of a B cell defect in patients with selective IgA deficiency. Clin Exp Immunol. 1979 Feb;35(2):296–305. [PMC free article] [PubMed] [Google Scholar]
  3. Cooper D. A., Duckett M., Petts V., Penny R. Corticosteroid enhancement of immunoglobulin synthesis by pokeweed mitogen-stimulated human lymphocytes. Clin Exp Immunol. 1979 Jul;37(1):145–151. [PMC free article] [PubMed] [Google Scholar]
  4. Delespesse G., Gausset P., Cauchie C., Govaerts A. Cellular aspects of selective IgA deficiency. Clin Exp Immunol. 1976 May;24(2):273–279. [PMC free article] [PubMed] [Google Scholar]
  5. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  6. Fauci A. S., Pratt K. R., Whalen G. Activation of human B lymphocytes. IV. Regulatory effects of corticosteroids on the triggering signal in the plaque-forming cell response of human peripheral blood B lymphocytes to polyclonal activation. J Immunol. 1977 Aug;119(2):598–603. [PubMed] [Google Scholar]
  7. Gupta S., Schwartz S. A., Good R. A. Subpopulations of human T lymphocytes. VII. Cellular basis of concanavalin A-induced T cell-mediated suppression of immunoglobulin production by B lymphocytes from normal humans. Cell Immunol. 1979 May;44(2):242–251. doi: 10.1016/0008-8749(79)90002-9. [DOI] [PubMed] [Google Scholar]
  8. Haynes B. F., Fauci A. S. Activation of human B lymphocytes. III. Concanavalin A-induced generation of suppressor cells of the plaque-forming cell response of normal human B lymphocytes. J Immunol. 1977 Jun;118(6):2281–2287. [PubMed] [Google Scholar]
  9. Hong R., Ammann A. J. Selective absence of IgA. Autoimmune phenomena and autoimmune disease. Am J Pathol. 1972 Dec;69(3):491–498. [PMC free article] [PubMed] [Google Scholar]
  10. Janossy G., Gomez De La Concha E., Waxdal M. J., Platts-Mills T. The effects of purified mitogenic proteins (Pa-1 and Pa-2) from pokeweed on human T and B lymphocytes in vitro. Clin Exp Immunol. 1976 Oct;26(1):108–117. [PMC free article] [PubMed] [Google Scholar]
  11. Janossy G., Gomez de la Concha E., Luquetti A., Snajdr M. J., Waxdal M. J., Platts-Mills T. A. T-cell regulation of immunoglobulin synthesis and proliferation in pokeweed (Pa-1)-stimulated human lymphocyte cultures. Scand J Immunol. 1977;6(1-2):109–123. doi: 10.1111/j.1365-3083.1977.tb00326.x. [DOI] [PubMed] [Google Scholar]
  12. King M. A., Wells J. V., Nelson D. S. IgA synthesis by peripheral blood mononuclear cells from normal and selectively IgA deficient subjects. Clin Exp Immunol. 1979 Nov;38(2):306–315. [PMC free article] [PubMed] [Google Scholar]
  13. Lawton A. R., Royal S. A., Self K. S., Cooper M. D. IgA determinants on B-lymphocytes in patients with deficiency of circulating IgA. J Lab Clin Med. 1972 Jul;80(1):26–33. [PubMed] [Google Scholar]
  14. Levitt D., Cooper M. D. Immunoregulatory defects in a family with selective IgA deficiency. J Pediatr. 1981 Jan;98(1):52–58. doi: 10.1016/s0022-3476(81)80532-x. [DOI] [PubMed] [Google Scholar]
  15. Schwartz S. A. Heavy chain-specific suppression of immunoglobulin synthesis and secretion by lymphocytes from patients with selective IgA deficiency. J Immunol. 1980 Apr;124(4):2034–2041. [PubMed] [Google Scholar]
  16. Thomas Y., Sosman J., Irigoyen O., Friedman S. M., Kung P. C., Goldstein G., Chess L. Functional analysis of human T cell subsets defined by monoclonal antibodies. I. Collaborative T-T interactions in the immunoregulation of B cell differentiation. J Immunol. 1980 Dec;125(6):2402–2408. [PubMed] [Google Scholar]
  17. Waldmann T. A., Broder S., Krakauer R., Durm M., Meade B., Goldman C. Defect in IgA secretion and in IgA specific suppressor cells in patients with selective IgA deficiency. Trans Assoc Am Physicians. 1976;89:215–224. [PubMed] [Google Scholar]
  18. de la Concha E. G., Oldham G., Webster A. D., Asherson G. L., Platts-Mills T. A. Quantitative measurements of T- and B-cell function in "variable" primary hypogammaglobulinaemia: evidence for a consistent B-cell defect. Clin Exp Immunol. 1977 Feb;27(2):208–215. [PMC free article] [PubMed] [Google Scholar]

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