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. 1982 Aug;49(2):433–440.

IgA containing immune complexes in dogs bearing a spontaneous mammary adenocarcinoma.

J Balint Jr, T Nagai, Y Ikeda, K Meek, D S Terman
PMCID: PMC1536514  PMID: 6982137

Abstract

Sera from dogs with mammary adenocarcinoma were assessed for the presence of immune complexes (IC) and the physicochemical composition of these complexes was investigated. Employing 125I-anti-canine IgG as indicator, elevated levels of C1q binding IgG were detected in sera of dogs with mammary adenocarcinoma. Polyacrylamide gel electrophoresis (PAGE) analysis of IC isolated by G-200 fractionation and protein A affinity chromatography revealed the presence of a dense polypeptide band corresponding to the alpha chain of IgA which was present in the mammary adenocarcinoma sera but not in normal dog sera or sera from dogs with other tumours. Employing monospecific radiolabelled anti-canine IgA as indicator in solid phase C1q binding radioimmunoassays, significantly elevated levels of C1q binding IgA were detected in five of eight mammary adenocarcinoma sera but not in sera of normal dogs or other tumour bearing dogs (P less than 0.05). Sera from mammary adenocarcinoma bearing dogs treated with 5% polyethylene glycol (PEG) and subjected to sucrose density gradient ultracentrifugation revealed IgA containing IC in fractions greater than 7S to greater than 19S. Findings suggest that IC are present in sera of dogs with mammary adenocarcinoma and that that IgA is a major and unique component of these complexes and, hence, may play a significant role in the development and evolution of the canine immune response to mammary adenocarcinoma.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balint J. P., Jr, Ikeda Y., Nagai T., Terman D. S. Isolation of human and canine IgM utilizing protein A affinity chromatography. Immunol Commun. 1981;10(6):533–540. doi: 10.3109/08820138109055703. [DOI] [PubMed] [Google Scholar]
  2. Balint J. R. Immune complexes with antiglobulin activity in sera of Moloney sarcoma-bearing rats. Clin Exp Immunol. 1982 Apr;48(1):70–78. [PMC free article] [PubMed] [Google Scholar]
  3. Hall R. P., Lawley T. J., Heck J. A., Katz S. I. IgA-containing circulating immune complexes in dermatitis herpetiformis, Henoch-Schönlein purpura, systemic lupus erythematosus and other diseases. Clin Exp Immunol. 1980 Jun;40(3):431–437. [PMC free article] [PubMed] [Google Scholar]
  4. Hoffken K., Meredith I. D., Robins R. A., Baldwin R. W., Davies C. J., Blamey R. W. Circulating immune complexes in patients with breast cancer. Br Med J. 1977 Jul 23;2(6081):218–220. doi: 10.1136/bmj.2.6081.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Howell-Saxton E., Wettstein F. O. Immunoglobulin M synthesized by human lymphoblastoid cells: interaction with Staphylococcus aureus and protein A. J Immunol. 1978 Oct;121(4):1334–1340. [PubMed] [Google Scholar]
  6. Jennette J. C., Feldman J. D. Sequential quantitation of circulating immune complexes in syngeneic and allogeneic rats bearing Moloney sarcomas. J Immunol. 1977 Jun;118(6):2269–2274. [PubMed] [Google Scholar]
  7. Jerry L. M., Rowden G., Cano P. O., Phillips T. M., Deutsch G. F., Capek A., Hartmann D., Lewis M. G. Immune complexes in human melanoma: a consequence of deranged immune regulation. Scand J Immunol. 1976;5(6-7):845–859. doi: 10.1111/j.1365-3083.1976.tb03034.x. [DOI] [PubMed] [Google Scholar]
  8. Kauffmann R. H., Herrmann W. A., Meÿer C. J., Daha M. R., Van Es L. A. Circulating IgA-immune complexes in Henoch-Schönlein purpura. A longitudinal study of their relationship to disease activity and vascular deposition of IgA. Am J Med. 1980 Dec;69(6):859–866. doi: 10.1016/s0002-9343(80)80011-8. [DOI] [PubMed] [Google Scholar]
  9. Kronvall G., Frommel D. Definition of staphylococcal protein A reactivity for human immunoglobulin G fragments. Immunochemistry. 1970 Jan;7(1):124–127. doi: 10.1016/0019-2791(70)90036-4. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Lewis M. G., Phillips T. M., Cook K. B., Blake J. Possible explanation for loss of detectable antibody in patients with disseminated malignant melanoma. Nature. 1971 Jul 2;232(5305):52–54. doi: 10.1038/232052a0. [DOI] [PubMed] [Google Scholar]
  13. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  14. Roberts-Thomson P. J., Kemp A. S. IgA and temperature dependent IgG complex formation in a patient with Waldenström's hypergammaglobulinaemic purpura. Clin Exp Immunol. 1980 Jan;39(1):164–169. [PMC free article] [PubMed] [Google Scholar]
  15. Rossen R. D., Reisberg M. A., Hersh E. M., Gutterman J. U. The C1q binding test for soluble immune complexes: clinical correlations obtained in patients with cancer. J Natl Cancer Inst. 1977 May;58(5):1205–1215. doi: 10.1093/jnci/58.5.1205. [DOI] [PubMed] [Google Scholar]
  16. Strandberg J. D., Goodman D. G. Animal model of human disease: canine mammary neoplasia. Am J Pathol. 1974 Apr;75(1):225–228. [PMC free article] [PubMed] [Google Scholar]
  17. Terman D. S., Moore D., Collins J., Johnston B., Person D., Templeton J., Poser R., Quinby F. Detection of immune complexes in sera of dogs with rheumatic and neoplastic diseases by 125I-Clq binding test. J Comp Pathol. 1979 Apr;89(2):221–227. doi: 10.1016/0021-9975(79)90061-6. [DOI] [PubMed] [Google Scholar]
  18. Terman D. S., Yamamoto T., Mattioli M., Cook G., Tillquist R., Henry J., Poser R., Daskal Y. Extensive necrosis of spontaneous canine mammary adenocarcinoma after extracorporeal perfusion over Staphylococcus aureus Cowans I. I. Description of acute tumoricidal response: morphologic, histologic, immunohistochemical, immunologic, and serologic findings. J Immunol. 1980 Feb;124(2):795–805. [PubMed] [Google Scholar]
  19. Yonemasu K., Stroud R. M. Clq: rapid purification method for preparation of monospecific antisera and for biochemical studies. J Immunol. 1971 Feb;106(2):304–313. [PubMed] [Google Scholar]

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