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. 1982 Apr;48(1):163–170.

The effects of T-cell growth factor and virus purification on virus-mediated inhibition of lymphocyte mitogenesis.

M A Wainberg, R G Margolese
PMCID: PMC1536554  PMID: 6282509

Abstract

Many different types of virus particles are able to non-specifically impede the ability of human peripheral blood lymphocytes to respond to mitogenic ro alloantigenic stimuli. This result is not obtained if ultra-purified virus is employed, although virus which has been banded only once through sucrose generally retains inhibitory potential. Ultra-pure virus is relatively unable to bind to cell surfaces, suggesting the importance of physical contact between viruses and cells in order for the observed inhibition to occur. Addition of exogenous T-cell growth factor (TCGF) to cultures containing virus, cells and stimulus causes a dose-dependent reversal of the usual inhibitory effect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolognesi D. P. Structural components of rna tumor viruses. Adv Virus Res. 1974;19:315–359. doi: 10.1016/s0065-3527(08)60663-6. [DOI] [PubMed] [Google Scholar]
  2. Böyum A. Isolation of leucocytes from human blood. A two-phase system for removal of red cells with methylcellulose as erythrocyte-aggregating agent. Scand J Clin Lab Invest Suppl. 1968;97:9–29. [PubMed] [Google Scholar]
  3. Caulfield M. J., Cerny J. Cell interactions in leukemia-associated immunosuppression: suppression of thymus-independent antibody responses by leukemia spleen cells (Moloney) in vitro is mediated by normal T cells. J Immunol. 1980 Jan;124(1):255–260. [PubMed] [Google Scholar]
  4. Fontana A., Weiner H. L. Interaction of reovirus with cell surface receptors. II. Generation of suppressor T cells by the hemagglutinin of reovirus type 3. J Immunol. 1980 Dec;125(6):2660–2664. [PubMed] [Google Scholar]
  5. Gillis S., Smith K. A. Long term culture of tumour-specific cytotoxic T cells. Nature. 1977 Jul 14;268(5616):154–156. doi: 10.1038/268154a0. [DOI] [PubMed] [Google Scholar]
  6. Gorczynski R. M. Immunity to murine sarcoma virus-inducted tumors. II. Suppression of T cell-mediated immunity by cells from progressor animals. J Immunol. 1974 May;112(5):1826–1838. [PubMed] [Google Scholar]
  7. Israel E., Wainberg M. A. Viral inhibition of lymphocyte mitogenesis: the role of macrophages as primary targets of virus-cell interaction. J Reticuloendothel Soc. 1981 Feb;29(2):105–116. [PubMed] [Google Scholar]
  8. Israel E., Yu M., Wainberg M. A. Non-specific effects of avian retrovirus co-incubation on lymphocyte function: abrogation of antigen- and mitogen-induced proliferative responsiveness. Immunology. 1979 Sep;38(1):41–50. [PMC free article] [PubMed] [Google Scholar]
  9. Kapusta M. A. The virus-activated suppressor cell hypothesis in experimental and human rheumatic diseases. J Rheumatol. 1980 May-Jun;7(3):309–315. [PubMed] [Google Scholar]
  10. Kirchner H., Chused T. M., Herberman R. B., Holden H. T., Lavrin D. H. Evidence of suppressor cell activity in spleens of mice bearing primary tumors induced by Moloney sarcoma virus. J Exp Med. 1974 Jun 1;139(6):1473–1487. doi: 10.1084/jem.139.6.1473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kupers T. A., Petrich J. M., Holloway A. W., St Geme J. W., Jr Depression of tuberculin delayed hypersensitivity by live attenuated mumps virus. J Pediatr. 1970 May;76(5):716–721. doi: 10.1016/s0022-3476(70)80290-6. [DOI] [PubMed] [Google Scholar]
  12. Livingston D. M., Parks W. P., Scolnick E. M., Ross J. Affinity chromatography of avian type C viral reverse transcriptase: studies with Rous sarcoma virus transformed rat cells. Virology. 1972 Nov;50(2):388–395. doi: 10.1016/0042-6822(72)90390-x. [DOI] [PubMed] [Google Scholar]
  13. Margolese R. G., Israel E., Wainberg M. A. Non-specific inhibition by virus particles of human lymphocytes mitogenesis. Clin Exp Immunol. 1980 Aug;41(2):243–251. [PMC free article] [PubMed] [Google Scholar]
  14. Olson G. B., Dent P. B., Rawls W. E., South M. A., Montgomery J. R., Melnick J. L., Good R. A. Abnormalities of in vitro lymphocyte responses during rubella virus infections. J Exp Med. 1968 Jul 1;128(1):47–68. doi: 10.1084/jem.128.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Poiesz B. J., Ruscetti F. W., Mier J. W., Woods A. M., Gallo R. C. T-cell lines established from human T-lymphocytic neoplasias by direct response to T-cell growth factor. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6815–6819. doi: 10.1073/pnas.77.11.6815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rogers H. J. Iron-binding catechols and virulence in Escherichia coli. Infect Immun. 1973 Mar;7(3):438–444. doi: 10.1128/iai.7.3.438-444.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ruscetti F. W., Gallo R. C. Human T-lymphocyte growth factor: regulation of growth and function of T lymphocytes. Blood. 1981 Mar;57(3):379–394. [PubMed] [Google Scholar]
  18. Ruscetti F. W., Morgan D. A., Gallo R. C. Functional and morphologic characterization of human T cells continuously grown in vitro. J Immunol. 1977 Jul;119(1):131–138. [PubMed] [Google Scholar]
  19. STARR S., BERKOVICH S. EFFECTS OF MEASLES, GAMMA-GLOBULIN-MODIFIED MEASLES AND VACCINE MEASLES ON THE TUBERCULIN TEST. N Engl J Med. 1964 Feb 20;270:386–391. doi: 10.1056/NEJM196402202700802. [DOI] [PubMed] [Google Scholar]
  20. Smith K. A., Gilbride K. J., Favata M. F. Lymphocyte activating factor promotes T-cell growth factor production by cloned murine lymphoma cells. Nature. 1980 Oct 30;287(5785):853–855. doi: 10.1038/287853a0. [DOI] [PubMed] [Google Scholar]
  21. Soontiëns F. J., van der Veen J. Evidence for a macrophage-mediated effect of poliovirus on the lymphocyte response to phytohemagglutinin. J Immunol. 1973 Nov;111(5):1411–1419. [PubMed] [Google Scholar]
  22. Stenson W. F., Parker C. W. Prostaglandins, macrophages, and immunity. J Immunol. 1980 Jul;125(1):1–5. [PubMed] [Google Scholar]
  23. TEMIN H. M., RUBIN H. Characteristics of an assay for Rous sarcoma virus and Rous sarcoma cells in tissue culture. Virology. 1958 Dec;6(3):669–688. doi: 10.1016/0042-6822(58)90114-4. [DOI] [PubMed] [Google Scholar]
  24. Umetsu D. T., Romano T. J., Bloom B. R., Thorbecke G. J. Diminution by vesicular stomatitis virus of acute graft vs host mortality in mice. Cell Immunol. 1979 Sep 1;46(2):416–421. doi: 10.1016/0008-8749(79)90429-5. [DOI] [PubMed] [Google Scholar]
  25. Wainberg M. A., Howe C. Infection-mediated resistance to cell fusion by inactive Sendai virus. Proc Soc Exp Biol Med. 1973 Mar;142(3):981–987. doi: 10.3181/00379727-142-37158. [DOI] [PubMed] [Google Scholar]
  26. Wainberg M. A., Israel E. Viral inhibition of lymphocyte mitogenesis. I. Evidence for the nonspecificity of the effect. J Immunol. 1980 Jan;124(1):64–70. [PubMed] [Google Scholar]
  27. Walzer P. D., Perl D. P., Krogstad D. J., Rawson P. G., Schultz M. G. Pneumocystis carinii pneumonia in the United States. Epidemiologic, diagnostic, and clinical features. Ann Intern Med. 1974 Jan;80(1):83–93. doi: 10.7326/0003-4819-80-1-83. [DOI] [PubMed] [Google Scholar]
  28. Willems F. T., Melnick J. L., Rawls W. E. Viral inhibition of the phytohemagglutinin response of human lymphocytes and application to viral hepatitis. Proc Soc Exp Biol Med. 1969 Feb;130(2):652–661. doi: 10.3181/00379727-130-33628. [DOI] [PubMed] [Google Scholar]
  29. Woodruff J. F., Woodruff J. J. T lymphocyte interaction with viruses and virus-infected tissues. Prog Med Virol. 1975;19:120–160. [PubMed] [Google Scholar]
  30. van Loon A. M., van der Logt J. T., van der Veen J. Poliovirus-induced suppression of lymphocyte stimulation: a macrophage-mediated effect. Immunology. 1979 May;37(1):135–143. [PMC free article] [PubMed] [Google Scholar]

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