Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1982 Apr;48(1):102–110.

Analysis of immunoglobulin G in multiple sclerosis brain: quantitative and isoelectric focusing studies.

P Glynn, H M Gilbert, J Newcombe, M L Cuzner
PMCID: PMC1536569  PMID: 7083640

Abstract

Immunoglobulin G (IgG) in soluble and particulate fractions of discrete tissue samples from multiple sclerosis (MS) brain was analysed. Supernatant IgG/albumin rations and particulate-bound IgG levels were highest in samples dissected from MS plaques and adjacent white matter. Acid extracts of particulate fractions from the equivalent of 1 g of MS plaque tissue contained up to 15 micrograms IgG, an order of magnitude less than the amount in extracts from a subacute sclerosing panencephatitis (SSPE) brain, but 20 times more than those from control brain. By contrast, supernatant fractions from SSPE brain and some MS plaques contained comparable amounts (100-200 micrograms/g tissue) of IgG, which were 10 times greater than those from control brain. Samples were subjected to isoelectric focusing (IEF), and IgG was visualized by immunoperoxidase staining. The IEF patterns of IgG from control samples were diffuse but samples from demyelinated MS tissue displayed distinct oligoclonal bands of IgG. A number of common IgG bands were apparent in extracts of supernatant and particulate fractions from the same plaque. The IEF spectra of plaque samples from three MS brains were different. Furthermore, quantitative variations in certain IgG bands were observed in different plaques from the same brain.

Full text

PDF
102

Images in this article

107Fig. 3
on p.107

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aarli J. A., Aparicio S. R., Lumsden C. E., Tönder O. Binding of normal human IgG to myelin sheaths, glia and neurons. Immunology. 1975 Jan;28(1):171–185. [PMC free article] [PubMed] [Google Scholar]
  2. Delmotte P., Gonsette R. Biochemical findings in multiple sclerosis IV. Isoelectric focusing of the CSF gamma globulins in multiple sclerosis (262 cases) and other neurological diseases (272 cases). J Neurol. 1977 Apr 28;215(1):27–37. doi: 10.1007/BF00312547. [DOI] [PubMed] [Google Scholar]
  3. Esiri M. M. Multiple sclerosis: a quantitative and qualitative study of immunoglobulin-containing cells in the central nervous system. Neuropathol Appl Neurobiol. 1980 Jan-Feb;6(1):9–21. doi: 10.1111/j.1365-2990.1980.tb00199.x. [DOI] [PubMed] [Google Scholar]
  4. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  5. Kjellin K. G., Vesterberg O. Isoelectric focusing of CSF proteins in neurological diseases. J Neurol Sci. 1974 Oct;23(2):199–213. doi: 10.1016/0022-510x(74)90223-8. [DOI] [PubMed] [Google Scholar]
  6. Kronvall G., Quie P. G., Williams R. C., Jr Quantitation of staphylococcal protein A: Determination of equilibrium constant and number of protein A residues on bacteria. J Immunol. 1970 Feb;104(2):273–278. [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Langone J. J. [125I]protein A: a tracer for general use in immunoassay. J Immunol Methods. 1978;24(3-4):269–285. doi: 10.1016/0022-1759(78)90131-x. [DOI] [PubMed] [Google Scholar]
  9. Mattson D. H., Roos R. P., Arnason B. G. Isoelectric focusing of IgG eluted from multiple sclerosis and subacute sclerosing panencephalitis brains. Nature. 1980 Sep 25;287(5780):335–337. doi: 10.1038/287335a0. [DOI] [PubMed] [Google Scholar]
  10. Nordal H. J., Vandvik B., Norrby E. Demonstration of electrophoretically restricted virus-specific antibodies in serum and cerebrospinal fluid by imprint electroimmunofixation. Scand J Immunol. 1978;7(5):381–388. doi: 10.1111/j.1365-3083.1978.tb00468.x. [DOI] [PubMed] [Google Scholar]
  11. Prineas J. W., Wright R. G. Macrophages, lymphocytes, and plasma cells in the perivascular compartment in chronic multiple sclerosis. Lab Invest. 1978 Apr;38(4):409–421. [PubMed] [Google Scholar]
  12. Ryberg B. Multiple specificities of antibrain antibodies in multiple sclerosis and chronic myelopathy. J Neurol Sci. 1978 Oct;38(3):357–382. doi: 10.1016/0022-510x(78)90142-9. [DOI] [PubMed] [Google Scholar]
  13. Sidén A. Crossed immunoelectrofocusing of cerebrospinal fluid immunoglobulins. J Neurol. 1977 Dec 13;217(2):103–109. doi: 10.1007/BF00312923. [DOI] [PubMed] [Google Scholar]
  14. Simpson J. F., Tourtellotte W. W., Kokmen E., Parker J. A., Itabashi H. H. Fluorescent protein tracing in multiple sclerosis brain tissue. Arch Neurol. 1969 Apr;20(4):373–377. doi: 10.1001/archneur.1969.00480100049007. [DOI] [PubMed] [Google Scholar]
  15. Tanaka R., Iwasaki Y., Koprowski H. Ultrastructural studies of perivascular cuffing cells in multiple sclerosis brain. Am J Pathol. 1975 Dec;81(3):467–478. [PMC free article] [PubMed] [Google Scholar]
  16. Tavolato B. F. Immunoglobulin G distribution in multiple sclerosis brain. An immunofluorescence study. J Neurol Sci. 1975 Jan;24(1):1–11. doi: 10.1016/0022-510x(75)90002-7. [DOI] [PubMed] [Google Scholar]
  17. Tourtellotte W. W., Ma B. I. Multiple sclerosis: the blood-brain-barrier and the measurement of de novo central nervous system IgG synthesis. Neurology. 1978 Sep;28(9 Pt 2):76–83. doi: 10.1212/wnl.28.9_part_2.76. [DOI] [PubMed] [Google Scholar]
  18. Tourtellotte W. On cerebrospinal fluid immunoglobulin-G (IgG) quotients in multiple sclerosis and other diseases. A review and a new formula to estimate the amount of IgG synthesized per day by the central nervous system. J Neurol Sci. 1970 Mar;10(3):279–304. doi: 10.1016/0022-510x(70)90156-5. [DOI] [PubMed] [Google Scholar]
  19. Traugott U., Snyder S., Raine C. S. Oligodendrocyte staining by multiple sclerosis serum is nonspecific. Ann Neurol. 1979 Jul;6(1):13–20. doi: 10.1002/ana.410060104. [DOI] [PubMed] [Google Scholar]
  20. Vandvik B., Norrby E. Oligoclonal IgG antibody response in the central nervous system to different measles virus antigens in subacute sclerosing panencephalitis. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1060–1063. doi: 10.1073/pnas.70.4.1060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vidal M. A., Conde F. P. Studies of the IgM and IgA contamination obtained by eluting IgG from protein A-Sepharose column with pH steps. J Immunol Methods. 1980;35(1-2):169–172. doi: 10.1016/0022-1759(80)90161-1. [DOI] [PubMed] [Google Scholar]
  22. Weil M. L., Norrby E., Itabashi H. H., Wilson R., Tourtellotte W. W., Heiner D. C. Immunoglobulin G from subacute sclerosing panencephalitis brain as an immunological reagent. Infect Immun. 1979 Apr;24(1):202–210. doi: 10.1128/iai.24.1.202-210.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES