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. 1982 Jul;49(1):41–49.

Spontaneous plaque forming cells in the peripheral blood of patients with systemic lupus erythematosus.

A G Wangel, A Milton, J B Egan
PMCID: PMC1536661  PMID: 6751633

Abstract

A reverse haemolytic plaque assay using staphylococcal protein A coupled to sheep red blood cells was set up in Cunningham chambers. Using this method, the numbers of Ficoll-Hypaque isolated peripheral blood lymphocytes (PBL) secreting IgG, IgA or IgM without preceding culture or mitogen stimulation were estimated in patients with systemic lupus erythematosus (SLE) and control subjects. Seven patients with clinically inactive SLE at the time of the study had values similar to those of the control subjects. In contrast, eight patients who had clinically active SLE had markedly increased numbers of PBL secreting IgG, IgA and IgM. Control experiments confirmed that the plaques were due to Ig secretion by lymphoid cells rather than to immune complexes adsorbed onto Fc receptor bearing cells or to passively adsorbed Ig. The results confirm the expected polyclonal B cell activation in patients with SLE and serial measurements showed that clinical relapses occurred only when the numbers of immunoglobulin secreting cells were high. Experiments in three patients with active SLE using native DNA prepared from T2 bacteriophage as the 'developing antigen' suggest that PBL secreting nDNA antibody can also be demonstrated by this method.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andres G. A., Accinni L., Beiser S. M., Christian C. L., Cinotti G. A., Erlanger B. F., Hsu K. C., Seegal B. C. Localization of fluorescein-labeled antinucleoside antibodies in glomeruli of patients with active systemic lupus erythematosus nephritis. J Clin Invest. 1970 Nov;49(11):2106–2118. doi: 10.1172/JCI106428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Appel A. E., Sablay L. B., Golden R. A., Barland P., Grayzel A. I., Bank N. The effect of normalization of serum complement and anti-DNA antibody on the course of lupus nephritis: a two year prospective study. Am J Med. 1978 Feb;64(2):274–283. doi: 10.1016/0002-9343(78)90056-6. [DOI] [PubMed] [Google Scholar]
  3. Bird A. G., Britton S. A new approach to the study of human B lymphocyte function using an indirect plaque assay and a direct B cell activator. Immunol Rev. 1979;45:41–67. doi: 10.1111/j.1600-065x.1979.tb00272.x. [DOI] [PubMed] [Google Scholar]
  4. Blaese R. M., Grayson J., Steinberg A. D. Increased immunoglobulin-secreting cells in the blood of patients with active systemic lupus erythematosus. Am J Med. 1980 Sep;69(3):345–350. doi: 10.1016/0002-9343(80)90003-0. [DOI] [PubMed] [Google Scholar]
  5. Bresnihan B., Jasin H. E. Suppressor function of peripheral blood mononuclear cells in normal individuals and in patients with systemic lupus erythematosus. J Clin Invest. 1977 Jan;59(1):106–116. doi: 10.1172/JCI108607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Budman D. R., Merchant E. B., Steinberg A. D., Doft B., Gershwin M. E., Lizzio E., Reeves J. P. Increased spontaneous activity of antibody-forming cells in the peripheral blood of patients with active SLE. Arthritis Rheum. 1977 Apr;20(3):829–833. doi: 10.1002/art.1780200312. [DOI] [PubMed] [Google Scholar]
  7. Chubick A., Sontheimer R. D., Gilliam J. N., Ziff M. An appraisal of tests for native DNA antibodies in connective tissue diseases. Clinical usefulness of Crithidia luciliae assay. Ann Intern Med. 1978 Aug;89(2):186–192. doi: 10.7326/0003-4819-89-2-186. [DOI] [PubMed] [Google Scholar]
  8. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  9. Davis P., Cumming R. H., Verrier-Jones J. Relationship between anti-DNA antibodies complement consumption and circulating immune complexes in systemic lupus erythematosus. Clin Exp Immunol. 1977 May;28(2):226–232. [PMC free article] [PubMed] [Google Scholar]
  10. Ginsburg W. W., Finkelman F. D., Lipsky P. E. Circulating and pokeweed mitogen-induced immunoglobulin-secreting cells in systemic lupus erythematosus. Clin Exp Immunol. 1979 Jan;35(1):76–88. [PMC free article] [PubMed] [Google Scholar]
  11. Goding J. W. The chromic chloride method of coupling antigens to erythrocytes: definition of some important parameters. J Immunol Methods. 1976;10(1):61–66. doi: 10.1016/0022-1759(76)90007-7. [DOI] [PubMed] [Google Scholar]
  12. Grennan D. M., Moseley A., Sloane D., Pumphrey R., Dick W. C., Buchanan W. W. The significance of serial measurement of serum anti-native DNA antibodies and complement C3 and C4 components in the management of patients with systemic lupus erythematosus. Aust N Z J Med. 1977 Dec;7(6):625–629. doi: 10.1111/j.1445-5994.1977.tb02319.x. [DOI] [PubMed] [Google Scholar]
  13. Gronowicz E., Coutinho A., Melchers F. A plaque assay for all cells secreting Ig of a given type or class. Eur J Immunol. 1976 Aug;6(8):588–590. doi: 10.1002/eji.1830060812. [DOI] [PubMed] [Google Scholar]
  14. Honig S., Gorevic P., Weissmann G. C-reactive protein in systemic lupus erythematosus. Arthritis Rheum. 1977 Jun;20(5):1065–1070. doi: 10.1002/art.1780200505. [DOI] [PubMed] [Google Scholar]
  15. Horowitz S., Borcherding W., Moorthy A. V., Chesney R., Schulte-Wisserman H., Hong R. Induction of suppressor T cells in systemic lupus erythematosus by thymosin and cultured thymic epithelium. Science. 1977 Sep 2;197(4307):999–1001. doi: 10.1126/science.302032. [DOI] [PubMed] [Google Scholar]
  16. Horwitz D. A., Lobo P. I. Characterizaiton of two populations of human lymphocytes bearing easily detectable surface immunoglobulin. J Clin Invest. 1975 Dec;56(6):1464–1472. doi: 10.1172/JCI108227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jasin H. E., Ziff M. Immunoglobulin synthesis by peripheral blood cells in systemic lupus erythematosus. Arthritis Rheum. 1975 May-Jun;18(3):219–228. doi: 10.1002/art.1780180305. [DOI] [PubMed] [Google Scholar]
  18. Kennedy J. C., Axelrad M. A. An improved assay for haemolytic plaque-forming cells. Immunology. 1971 Feb;20(2):253–257. [PMC free article] [PubMed] [Google Scholar]
  19. Koffler D., Agnello V., Thoburn R., Kunkel H. G. Systemic lupus erythematosus: prototype of immune complex nephritis in man. J Exp Med. 1971 Sep 1;134(3):169–179. [PMC free article] [PubMed] [Google Scholar]
  20. Krakauer R. S., Clough J. D., Alexander T., Sundeen J., Sauder D. N. Suppressor cell defect in SLE: relationship to native DNA binding. Clin Exp Immunol. 1980 Apr;40(1):72–76. [PMC free article] [PubMed] [Google Scholar]
  21. Lebman D. A., Calkins C. E. Demonstration of active suppressor cells in spleens of young NZB mice. Cell Immunol. 1980 May;51(2):419–423. doi: 10.1016/0008-8749(80)90274-9. [DOI] [PubMed] [Google Scholar]
  22. Maddison P. J., Reichlin M. Deposition of antibodies to a soluble cytoplasmic antigen in the kidneys of patients with systemic lupus erythematosus. Arthritis Rheum. 1979 Aug;22(8):858–863. doi: 10.1002/art.1780220808. [DOI] [PubMed] [Google Scholar]
  23. Manny N., Datta S. K., Schwartz R. S. Synthesis of IgM by cells of NZB and SWR mice and their crosses. J Immunol. 1979 Apr;122(4):1220–1227. [PubMed] [Google Scholar]
  24. Miller K. B., Schwartz R. S. Familial abnormalities of suppressor-cell function in systemic lupus erythematosus. N Engl J Med. 1979 Oct 11;301(15):803–809. doi: 10.1056/NEJM197910113011502. [DOI] [PubMed] [Google Scholar]
  25. Muchmore A. V., Koski I., Dooley N., Blaese R. M. Artifactual plaque formation in vitro and in vivo to passive transfer of specific antibody. J Immunol. 1976 Apr;116(4):1016–1019. [PubMed] [Google Scholar]
  26. Nies K. M., Louie J. S. Impaired immunoglobulin synthesis by peripheral blood lymphocytes in systemic lupus erythematosus. Arthritis Rheum. 1978 Jan-Feb;21(1):51–57. doi: 10.1002/art.1780210109. [DOI] [PubMed] [Google Scholar]
  27. Okudaira K., Tanimoto K., Nakamura T., Horiuchi Y. Spontaneously enhanced in vitro immunoglobulin synthesis by B cells in systemic lupus erythematosus. Clin Immunol Immunopathol. 1980 Jul;16(3):267–278. doi: 10.1016/0090-1229(80)90132-4. [DOI] [PubMed] [Google Scholar]
  28. Primi D., Lewis G. K., Goodman J. W. A hemolytic plaque assay for activated murine T cells. J Exp Med. 1979 Oct 1;150(4):987–1000. doi: 10.1084/jem.150.4.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Pryjma J., Muñoz J., Virella G., Fudenberg H. H. Evaluation of IgM, IgG, IgA, IgD, and IgE secretion by human peripheral blood lymphocytes in cultures stimulated with pokeweed mitogen and Staphylococcus aureus Cowan I. Cell Immunol. 1980 Mar 1;50(1):115–124. doi: 10.1016/0008-8749(80)90011-8. [DOI] [PubMed] [Google Scholar]
  30. Sagawa A., Abdou N. I. Suppressor-cell dysfunction in systemic lupus erythematosus. Cells involved and in vitro correction. J Clin Invest. 1978 Oct;62(4):789–796. doi: 10.1172/JCI109190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sakane T., Steinberg A. D., Green I. Studies of immune functions of patients with systemic lupus erythematosus. V. T cell suppressor function and autologous mixed lymphocyte reaction during active and inactive phases of disease. Arthritis Rheum. 1980 Feb;23(2):225–231. doi: 10.1002/art.1780230214. [DOI] [PubMed] [Google Scholar]
  32. Strelkauskas A. J., Callery R. T., McDowell J., Borel Y., Schlossman S. F. Direct evidence for loss of human suppressor cells during active autoimmune disease. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5150–5154. doi: 10.1073/pnas.75.10.5150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Webb J. Clinico-laboratory aspects of anti-nuclear and anti-native DNA antibody tests. Aust N Z J Med. 1978;8 (Suppl 1):61–67. doi: 10.1111/j.1445-5994.1978.tb04787.x. [DOI] [PubMed] [Google Scholar]
  34. Winchester R. J., Winfield J. B., Siegal F., Wernet P., Bentwich Z., Kunkel H. G. Analyses of lymphocytes from patients with rheumatoid arthritis and systemic lupus erythematosus. Occurrence of interfering cold-reactive antilymphocyte antibodies. J Clin Invest. 1974 Nov;54(5):1082–1092. doi: 10.1172/JCI107852. [DOI] [PMC free article] [PubMed] [Google Scholar]

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