Abstract
The cells in the K-562 line have been shown to express Fc receptors as demonstrated by rosette formation with sheep erythrocytes (E) sensitized with haemagglutinin (EA). Rosette formation is inhibited by prior incubation of the cells with goat or monkey anti-K-562 serum, gammaglobulin fraction, or Fab fragments. Alkaline aggregated human IgG also inhibits rosette formation. Furthermore, formed rosettes can be dissociated by goat anti-K-562 gammaglobulins indicating that the binding of the ligand to one entity interferes with the binding to the other. We also found that treatment of K-562 cells with specific anti-K-562 globulin leads to patching and capping of surface antigen. After patching and capping, the cells will not form rosettes. These findings suggest that the Fc receptor and the K-562 associated antigen are either located very close to one another or are the same entity.
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Selected References
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- Collins J. L., Wust C. J., Lozzio B. B., Lozzio C. B. Characterization of an antigen from the myelogenous leukemia cell line K-562. J Natl Cancer Inst. 1977 Dec;59(6):1667–1674. doi: 10.1093/jnci/59.6.1667. [DOI] [PubMed] [Google Scholar]
- Gattringer C., Wick G. Relationship between E receptors and a T-specific surface antigen on human T cells. Immunology. 1977 Feb;32(2):199–205. [PMC free article] [PubMed] [Google Scholar]
- Lozzio B. B., Lozzio C. B., Bamberger E. G., Feliu A. S. A multipotential leukemia cell line (K-562) of human origin. Proc Soc Exp Biol Med. 1981 Apr;166(4):546–550. doi: 10.3181/00379727-166-41106. [DOI] [PubMed] [Google Scholar]
- Lozzio B. B., Lozzio C. B., Krauss S., Wust C. J., Girardi A. Leukemia-associated antigens detected by a nonhuman primate antiserum to a Ph1+ myeologenous leukemia cell line. Blood. 1977 Jul;50(1):115–124. [PubMed] [Google Scholar]
- Lozzio B. B., Lozzio C. B. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk Res. 1979;3(6):363–370. doi: 10.1016/0145-2126(79)90033-x. [DOI] [PubMed] [Google Scholar]
- Lozzio C. B., Lozzio B. B., Yang W. K., Ichiki A. T., Bamberger E. G. Absence of thymus-derived lymphocyte markers in myelogenous leukemia (Ph1+) cell line K-562. Cancer Res. 1976 Dec;36(12):4657–4662. [PubMed] [Google Scholar]
- Walker L., Hay F. C., Roitt I. M. Characteristics of complexes for arming and inhibiting effector cells for antibody-dependent cell-mediated cytotoxicity. Clin Exp Immunol. 1979 Jun;36(3):397–407. [PMC free article] [PubMed] [Google Scholar]
- Wust C. J., Green M., Lozzio C. B., Lozzio B. B. Antibody-dependent cytolysis of the human leukemia cell line K-562 in the absence of effector cells or complement. Blood. 1982 Jan;59(1):133–140. [PubMed] [Google Scholar]