Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1982 Sep;49(3):670–676.

Functional assessment of T and B lymphocytes in patients with selective IgM deficiency.

E G De la Concha, M C Garcia-Rodriguez, J M Zabay, M T Laso, F Alonso, A Bootello, G Fontan
PMCID: PMC1536738  PMID: 6983403

Abstract

Two patients with selective IgM deficiency were studied. Both presented with dermatitis, chronic diarrhoea, recurrent respiratory infections, failure to thrive, elevated serum IgE levels and in vivo impairment of antibody production. No phagocytic or complement abnormalities were found. B lymphocytes with surface IgM were present in normal or high percentage in peripheral blood, and produced normal amounts of IgM in vitro when co-cultured with normal T cells. Patients' T cells did not show excess suppressor function in vitro but had a decreased helper activity for IgM, IgG and IgA production. It is suggested that both patients have an extensive humoral immune deficiency that might be caused by the immunoregulatory T cell defect.

Full text

PDF
670

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cassidy J. T., Oldham G., Platts-Mills T. A. Functional assessment of a B cell defect in patients with selective IgA deficiency. Clin Exp Immunol. 1979 Feb;35(2):296–305. [PMC free article] [PubMed] [Google Scholar]
  2. De la Concha E. G., Subiza J. L., Fontán G., Pascual-Salcedo D., Sequí J., Bootello A. Disorders of regulatory T cells in patients with selective IgA deficiency and its relationship to associated autoimmune phenomena. Clin Exp Immunol. 1982 Aug;49(2):410–418. [PMC free article] [PubMed] [Google Scholar]
  3. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
  4. Fauci A. S., Pratt K. R., Whalen G. Activation of human B lymphocytes. IV. Regulatory effects of corticosteroids on the triggering signal in the plaque-forming cell response of human peripheral blood B lymphocytes to polyclonal activation. J Immunol. 1977 Aug;119(2):598–603. [PubMed] [Google Scholar]
  5. Fontan G., Lorente F., Garcia Rodriguez M. C., Ojeda J. A. Defective neutrophil chemotaxis and hyperimmunoglobulinemia E-a reversible defect? Acta Paediatr Scand. 1976 Jul;65(4):509–511. doi: 10.1111/j.1651-2227.1976.tb04922.x. [DOI] [PubMed] [Google Scholar]
  6. Gelfand J. A., Fauci A. S., Green I., Frank M. M. A simple method for the determination of complement receptor-bearing mononuclear cells. J Immunol. 1976 Mar;116(3):595–599. [PubMed] [Google Scholar]
  7. Hobbs J. R., Milner R. D., Watt P. J. Gamma-M deficiency predisposing to meningococcal septicaemia. Br Med J. 1967 Dec 9;4(5579):583–586. doi: 10.1136/bmj.4.5579.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Holt G. H., Turner K. J., Holt B. J. In vitro synthesis of IgE by human peripheral blood leucocytes. II modulation of IgE B cell activity by isotype-specific regulatory T cells. Clin Exp Immunol. 1981 Sep;45(3):523–531. [PMC free article] [PubMed] [Google Scholar]
  9. Janossy G., Gomez De La Concha E., Waxdal M. J., Platts-Mills T. The effects of purified mitogenic proteins (Pa-1 and Pa-2) from pokeweed on human T and B lymphocytes in vitro. Clin Exp Immunol. 1976 Oct;26(1):108–117. [PMC free article] [PubMed] [Google Scholar]
  10. Lampson L. A., Levy R. Two populations of Ia-like molecules on a human B cell line. J Immunol. 1980 Jul;125(1):293–299. [PubMed] [Google Scholar]
  11. Lobo P. I., Westervelt F. B., Horwitz D. A. Identification of two populations of immunoglobulin-bearing lymphocytes in man. J Immunol. 1975 Jan;114(1 Pt 1):116–119. [PubMed] [Google Scholar]
  12. Moretta L., Ferrarini M., Mingari M. C., Moretta A., Webb S. R. Subpopulations of human T cells identified by receptors for immunoglobulins and mitogen responsiveness. J Immunol. 1976 Dec;117(6):2171–2174. [PubMed] [Google Scholar]
  13. Preisler H. D., Hasenclever H. F., Levitan A. A., Henderson E. S. Serologic diagnosis of disseminated candidiasis in patients with acute leukemia. Ann Intern Med. 1969 Jan;70(1):19–30. doi: 10.7326/0003-4819-70-1-19. [DOI] [PubMed] [Google Scholar]
  14. Record C. O., Shilkin K. B., Eddleston A. L., Williams R. Intrahepatic sclerosing cholangitis associated with a familial immunodeficiency syndrome. Lancet. 1973 Jul 7;2(7819):18–20. doi: 10.1016/s0140-6736(73)91949-1. [DOI] [PubMed] [Google Scholar]
  15. Reinherz E. L., Schlossman S. F. The differentiation and function of human T lymphocytes. Cell. 1980 Apr;19(4):821–827. doi: 10.1016/0092-8674(80)90072-0. [DOI] [PubMed] [Google Scholar]
  16. Royston I., Majda J. A., Baird S. M., Meserve B. L., Griffiths J. C. Human T cell antigens defined by monoclonal antibodies: the 65,000-dalton antigen of T cells (T65) is also found on chronic lymphocytic leukemia cells bearing surface immunoglobulin. J Immunol. 1980 Aug;125(2):725–731. [PubMed] [Google Scholar]
  17. Saxon A., Kobayashi R. H., Stevens R. H., Singer A. D., Stiehm E. R., Siegel S. C. In vitro analysis of humoral immunity in antibody deficiency with normal immunoglobulins. Clin Immunol Immunopathol. 1980 Oct;17(2):235–244. doi: 10.1016/0090-1229(80)90092-6. [DOI] [PubMed] [Google Scholar]
  18. Siegal F. P., Siegal M., Good R. A. Role of helper, suppressor and B-cell defects in the pathogenesis of the hypogammaglobulinemias. N Engl J Med. 1978 Jul 27;299(4):172–178. doi: 10.1056/NEJM197807272990404. [DOI] [PubMed] [Google Scholar]
  19. Stoelinga G. B., van Munster P. J. Antibody deficiency syndrome and autoimmune haemolytic anaemia in a boy with isolated IgM deficiency dysimmunoglobulinaemia type 5. Acta Paediatr Scand. 1969 Jul;58(4):352–362. doi: 10.1111/j.1651-2227.1969.tb04731.x. [DOI] [PubMed] [Google Scholar]
  20. Waldmann T. A., Broder S., Krakauer R., Durm M., Meade B., Goldman C. Defect in IgA secretion and in IgA specific suppressor cells in patients with selective IgA deficiency. Trans Assoc Am Physicians. 1976;89:215–224. [PubMed] [Google Scholar]
  21. Waldmann T. A., Durm M., Broder S., Blackman M., Blaese R. M., Strober W. Role of suppressor T cells in pathogenesis of common variable hypogammaglobulinaemia. Lancet. 1974 Sep 14;2(7881):609–613. doi: 10.1016/s0140-6736(74)91940-0. [DOI] [PubMed] [Google Scholar]
  22. Wybran J., Levin A. S., Spitler L. E., Fudenberg H. H. Rosette-forming cells, immunologic deficiency diseases and transfer factor. N Engl J Med. 1973 Apr 5;288(14):710–713. doi: 10.1056/NEJM197304052881405. [DOI] [PubMed] [Google Scholar]
  23. de la Concha E. G., Oldham G., Webster A. D., Asherson G. L., Platts-Mills T. A. Quantitative measurements of T- and B-cell function in "variable" primary hypogammaglobulinaemia: evidence for a consistent B-cell defect. Clin Exp Immunol. 1977 Feb;27(2):208–215. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES