Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1983 Jan;51(1):173–177.

The idiotypic specificities of lymphocytes in human monoclonal gammopathies: analysis with the fluorescence activated cell sorter.

A L Carmagnola, M Boccadoro, M Massaia, A Pileri
PMCID: PMC1536741  PMID: 6831769

Abstract

Four monoclonal IgG kappa gammopathies were studied with anti-idiotypic sera and the percentage of lymphocytes bearing such idiotypes was evaluated with a fluorescence activated cell sorter (FACS). A large percentage (10-15%) of peripheral lymphocytes bearing receptors with the same idiotypic specificities as the M component was observed in multiple myeloma (MM) and in monoclonal gammopathy of undetermined significance (MGUS), suggesting a malignant origin for both these diseases. Capping-endocytosis and resynthesis experiments showed that these idiotypic receptors were not simply adsorbed on the cell membrane, but actually synthesized by the cells bearing them. Experiments performed after effective conventional chemotherapy showed a strong reduction of the idiotypic compartment in only one MM patient.

Full text

PDF
173

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boccadoro M., Van Acker A., Pileri A., Urbain J. Idiotypic lymphocytes in human monoclonal gammopathies. Ann Immunol (Paris) 1981 Jan-Feb;132C(1):9–19. doi: 10.1016/0769-2625(81)90003-9. [DOI] [PubMed] [Google Scholar]
  2. Durie B. G., Salmon S. E. A clinical staging system for multiple myeloma. Correlation of measured myeloma cell mass with presenting clinical features, response to treatment, and survival. Cancer. 1975 Sep;36(3):842–854. doi: 10.1002/1097-0142(197509)36:3<842::aid-cncr2820360303>3.0.co;2-u. [DOI] [PubMed] [Google Scholar]
  3. Fu S. M., Winchester R. J., Feizi T., Walzer P. D., Kunkel H. G. Idiotypic specificity of surface immunoglobulin and the maturation of leukemic bone-marrow-derived lymphocytes. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4487–4490. doi: 10.1073/pnas.71.11.4487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hoover R. G., Hickman S., Gebel H. M., Rebbe N., Lynch R. G. Expansion of Fc receptor-bearing T lymphocytes in patients with immunoglobulin G and immunoglobulin A myeloma. J Clin Invest. 1981 Jan;67(1):308–311. doi: 10.1172/JCI110028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. King M. A., Wells J. V. Cell-bound immunoglobulin on peripheral blood mononuclear cells of patients with myeloma. Clin Exp Immunol. 1981 Sep;45(3):552–556. [PMC free article] [PubMed] [Google Scholar]
  6. Kubagawa H., Vogler L. B., Capra J. D., Conrad M. E., Lawton A. R., Cooper M. D. Studies on the clonal origin of multiple myeloma. Use of individually specific (idiotype) antibodies to trace the oncogenic event to its earliest point of expression in B-cell differentiation. J Exp Med. 1979 Oct 1;150(4):792–807. doi: 10.1084/jem.150.4.792. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kyle R. A. Monoclonal gammopathy of undetermined significance. Natural history in 241 cases. Am J Med. 1978 May;64(5):814–826. doi: 10.1016/0002-9343(78)90522-3. [DOI] [PubMed] [Google Scholar]
  8. Lea T., Førre O. T., Michaelsen T. E., Natvig J. B. Shared idiotypes of human peripheral blood B and T lymphocytes. J Immunol. 1979 Jun;122(6):2413–2417. [PubMed] [Google Scholar]
  9. Lindström F. D., Hardy W. R., Eberle B. J., Williams R. C., Jr Multiple myeloma and benign monoclonal gammopathy: differentiation by immunofluorescence of lymphocytes. Ann Intern Med. 1973 Jun;78(6):837–844. doi: 10.7326/0003-4819-78-6-837. [DOI] [PubMed] [Google Scholar]
  10. Lynch R. G., Rohrer J. W., Odermatt B., Gebel H. M., Autry J. R., Hoover R. G. Immunoregulation of murine myeloma cell growth and differentiation: a monoclonal model of B cell differentiation. Immunol Rev. 1979;48:45–80. doi: 10.1111/j.1600-065x.1979.tb00298.x. [DOI] [PubMed] [Google Scholar]
  11. Mellstedt H., Hammarström S., Holm G. Monoclonal lymphocyte population in human plasma cell myeloma. Clin Exp Immunol. 1974 Jul;17(3):371–384. [PMC free article] [PubMed] [Google Scholar]
  12. Mellstedt H., Pettersson D., Holm G. Monoclonal B-lymphocytes in peripheral blood of patients with plasma cell myeloma. Relation to activity of the disease. Scand J Haematol. 1976 Feb;16(2):112–120. doi: 10.1111/j.1600-0609.1976.tb01125.x. [DOI] [PubMed] [Google Scholar]
  13. Preud'homme J. L., Klein M., Labaume S., Seligmann M. Idiotype-bearing and antigen-binding receptors produced by blood T lymphocytes in a case of human myeloma. Eur J Immunol. 1977 Dec;7(12):840–846. doi: 10.1002/eji.1830071204. [DOI] [PubMed] [Google Scholar]
  14. Schedel I., Peest D., Stünkel K., Fricke M., Eckert G., Deicher H. Idiotype-bearing peripheral blood lymphocytes in human multiple myeloma and Waldenström's macroglobulinaemia. Scand J Immunol. 1980;11(4):437–444. doi: 10.1111/j.1365-3083.1980.tb00010.x. [DOI] [PubMed] [Google Scholar]
  15. Van Acker A., Conte F., Hulin N., Urbain J. Idiotypic studies on myeloma B cells. Eur J Cancer. 1979 May;15(5):627–635. doi: 10.1016/0014-2964(79)90136-1. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES