Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1983 Mar;51(3):605–610.

Cross-reactivity of IgA antibodies between renal mesangial areas and nuclei of tonsillar cells in patients with IgA nephropathy.

Y Tomino, H Sakai, M Endoh, T Suga, M Miura, H Kaneshige, Y Nomoto
PMCID: PMC1536797  PMID: 6851248

Abstract

A study on autoradiographical analysis of antigenic sites in patients with IgA nephropathy is described. Renal biopsy specimens were obtained from patients with IgA nephropathy. These specimens were treated with citrate buffer (pH 3.2) and the 'eluate' was neutralized by sodium hydroxide. The 'eluate' was labelled with 125iodine by the chloramine-T method. 125I-labelled eluate was then applied to the tonsillar cells obtained from the same and other patients with IgA nephropathy as well as to those with other glomerular diseases. The tonsillar cells were dipped into the emulsion (NBT-2) and then examined with a light microscope. It was demonstrated that the antibodies eluted from renal tissues of patients with IgA nephropathy specificially bound with the nuclear regions of tonsillar cells. The binding of eluted antibodies and tonsillar cells was completely inhibited by the addition of anti-human IgA antisera, but not inhibited by human IgA myeloma proteins. The eluted antibodies bound with tonsillar cells from the same patients, but only 10% of them bound with the tonsillar cells obtained from other patients with IgA nephropathy. It is concluded that IgA antibodies deposited in glomeruli specifically bind with tonsillar cells obtained from patients with IgA nephropathy and these antibodies show some heterogeneity among those patients.

Full text

PDF

Images in this article

608Fig. 1
on p.608

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. André C., André F., Fargier C. Distribution of IgA 1 and IgA 2 plasma cells in various normal human tissues and in the jejunum of plasma IgA-deficient patients. Clin Exp Immunol. 1978 Aug;33(2):327–331. [PMC free article] [PubMed] [Google Scholar]
  2. Berger J. IgA glomerular deposits in renal disease. Transplant Proc. 1969 Dec;1(4):939–944. [PubMed] [Google Scholar]
  3. Conley M. E., Cooper M. D., Michael A. F. Selective deposition of immunoglobulin A1 in immunoglobulin A nephropathy, anaphylactoid purpura nephritis, and systemic lupus erythematosus. J Clin Invest. 1980 Dec;66(6):1432–1436. doi: 10.1172/JCI109998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Finlayson G., Alexander R., Juncos L., Schlein E., Teague P., Waldman R., Cade R. Immunoglobulin A glomerulonephritis: a clinicopathologic study. Lab Invest. 1975 Feb;32(2):140–148. [PubMed] [Google Scholar]
  5. Kawamura N., Murakami Y., Ohkoshi M., Nomoto Y., Endoh M., Tomino Y., Sakai H. Modified open renal biopsy. Tokai J Exp Clin Med. 1980 Jan;5(1):23–27. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lowance D. C., Mullins J. D., McPhaul J. J., Jr IgA-Associated glomerulonepbritis. Int Rev Exp Pathol. 1977;17:143–172. [PubMed] [Google Scholar]
  8. MESSIER B., LEBLOND C. P. Preparation of coated radioautographs by dipping sections in fluid emulsion. Proc Soc Exp Biol Med. 1957 Oct;96(1):7–10. doi: 10.3181/00379727-96-23380. [DOI] [PubMed] [Google Scholar]
  9. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  10. McCoy R. C., Abramowsky C. R., Tisher C. C. IgA nephropathy. Am J Pathol. 1974 Jul;76(1):123–144. [PMC free article] [PubMed] [Google Scholar]
  11. Shirai T., Tomino Y., Sato M., Yoshiki T., Itoh T. IgA nephropathy: clinicopathology and immunopathology. Contrib Nephrol. 1978;9:88–100. doi: 10.1159/000401436. [DOI] [PubMed] [Google Scholar]
  12. Tomino Y., Endoh M., Nomoto Y., Sakai H. Specificity of eluted antibody from renal tissues of patients with IgA nephropathy. Am J Kidney Dis. 1982 Mar;1(5):276–280. doi: 10.1016/s0272-6386(82)80025-5. [DOI] [PubMed] [Google Scholar]
  13. Tomino Y., Nomoto Y., Endoh M., Sakai H. Evaluation of the staining conditions of immunofluorescence in primary glomerulonephritis. Acta Pathol Jpn. 1981 Jan;31(1):99–104. doi: 10.1111/j.1440-1827.1981.tb00988.x. [DOI] [PubMed] [Google Scholar]
  14. Whitworth J. A., Leibowitz S., Kennedy M. C., Cameron J. S., Chantler C. IgA and glomerular disease. Clin Nephrol. 1976 Jan;5(1):33–36. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES