Abstract
Lymphocyte activating factor (LAF) is a monocyte derived product which has the ability to enhance the proliferative response of murine thymocytes to the T cell mitogen concanavalin A (Con A). In this report, we have demonstrated that supernatants from normal human polymorphonuclear cells (PMNs) and from granules obtained from PMNs exhibit activity identical to LAF when tested against mouse thymocytes. Our results suggest that PMNs may release factors essential for T cell activation and thus, like monocytes, may serve as important accessory cells in the development of T cell-dependent immunity.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Böyum A. Isolation of leucocytes from human blood. A two-phase system for removal of red cells with methylcellulose as erythrocyte-aggregating agent. Scand J Clin Lab Invest Suppl. 1968;97:9–29. [PubMed] [Google Scholar]
- Dinarello C. A., Goldin N. P., Wolff S. M. Demonstration and characterization of two distinct human leukocytic pyrogens. J Exp Med. 1974 Jun 1;139(6):1369–1381. doi: 10.1084/jem.139.6.1369. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fitchen J. H., Foon K. A., Cline M. J. The antigenic characteristics of hematopoietic stem cells. N Engl J Med. 1981 Jul 2;305(1):17–25. doi: 10.1056/NEJM198107023050104. [DOI] [PubMed] [Google Scholar]
- Henson P. M. The adherence of leucocytes and platelets induced by fixed IgG antibody or complement. Immunology. 1969 Jan;16(1):107–121. [PMC free article] [PubMed] [Google Scholar]
- Koeffler H. P., Golde D. W. Chronic myelogenous leukemia--new concepts (first of two parts). N Engl J Med. 1981 May 14;304(20):1201–1209. doi: 10.1056/NEJM198105143042004. [DOI] [PubMed] [Google Scholar]
- Lachman L. B., Moore J. O., Metzgar R. S. Preparation and characterization of lymphocyte-activating factor (LAF) from acute monocytic and myelomonocytic leukemia cells. Cell Immunol. 1978 Nov;41(1):199–206. doi: 10.1016/s0008-8749(78)80040-9. [DOI] [PubMed] [Google Scholar]
- Mizel S. B., Oppenheim J. J., Rosenstreich D. L. Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line, P388D1. I. Enhancement of LAF production by activated T lymphocytes. J Immunol. 1978 May;120(5):1497–1503. [PubMed] [Google Scholar]
- Nakamura S., Yoshinaga M., Hayashi H. Interaction between lymphocytes and inflammatory exudate cells. II. A proteolytic enzyme released by PMN as a possible mediator for enhancement of thymocyte response. J Immunol. 1976 Jul;117(1):1–6. [PubMed] [Google Scholar]
- West B. C., Rosenthal A. S., Gelb N. A., Kimball H. R. Separation and characterization of human neutrophil granules. Am J Pathol. 1974 Oct;77(1):41–66. [PMC free article] [PubMed] [Google Scholar]