Abstract
The role of thiols in T cell-mediated cytotoxicity was investigated by studying the thiols of the target cell and cytotoxic cell separately. Agents which protect the reduced thiols of the target cell inhibit their lysis by the cytotoxic T cells; thiol reactive reagents may be directly toxic to the target cell. The thiol groups of the effector cell are also important, since pre-treatment with thiol reactive reagents inhibits killing.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berke G., Gabison D. Energy requirements of the binding and lytic steps of T lymphocyte-mediated cytolysis of leukemic cells in vitro. Eur J Immunol. 1975 Oct;5(10):671–675. doi: 10.1002/eji.1830051004. [DOI] [PubMed] [Google Scholar]
- Brunner K. T., Mauel J., Cerottini J. C., Chapuis B. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 1968 Feb;14(2):181–196. [PMC free article] [PubMed] [Google Scholar]
- Golstein P., Smith E. T. Mechanism of T-cell-mediated cytolysis: the lethal hit stage. Contemp Top Immunobiol. 1977;7:273–300. doi: 10.1007/978-1-4684-3054-7_8. [DOI] [PubMed] [Google Scholar]
- Guilbault G. G., Brignac P. J., Jr, Juneau M. New substrates for the fluorometric determination of oxidative enzymes. Anal Chem. 1968 Jul;40(8):1256–1263. doi: 10.1021/ac60264a027. [DOI] [PubMed] [Google Scholar]
- Redelman D., Hudig D. The mechanism of cell-mediated cytotoxicity. I. Killing by murine cytotoxic T lymphocytes requires cell surface thiols and activated proteases. J Immunol. 1980 Feb;124(2):870–878. [PubMed] [Google Scholar]
- Thorne K. J., Svvennsen R. J., Franks D. Role of hydrogen peroxide and peroxidase in the cytotoxicity of Trypanosoma dionisii by human granulocytes. Infect Immun. 1978 Sep;21(3):798–805. doi: 10.1128/iai.21.3.798-805.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thorne K. J., Svvennsen R. J., Franks D. Role of hydrogen peroxide in the cytotoxic reaction of T lymphocytes. Clin Exp Immunol. 1980 Feb;39(2):486–495. [PMC free article] [PubMed] [Google Scholar]