Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1982 Oct;50(1):183–188.

Enhancement of human ADCC with interferon.

R Warren, D Kalamasz, R Storb
PMCID: PMC1536858  PMID: 6184191

Abstract

We have studied the ability of a purified preparation of leucocyte interferon (IF) to augment human antibody-dependent cell-mediated cytotoxicity (ADCC). The IF preparation was added to cultures of the ADCC assay consisting of peripheral blood mononuclear target cells, a sensitizing human antiserum specific for the HLA antigens expressed on the target cells, and effector cells obtained from the peripheral blood of healthy human subjects. Increased cytolysis was found at each of four effector to target cell ratios but could be demonstrated only at concentrations of the sensitizing antiserum of 2.5% and lower. Removal of monocytes from the effector cell population did not decrease the level of cytotoxicity, and isolated monocytes were unreactive, suggesting that K cells (the subpopulation of lymphocytes mediating ADCC) were susceptible to augmentation. Since it is widely accepted that IF can augment spontaneous cytotoxicity mediated by natural killer (NK) cells, our results provide further evidence that human NK and K cells are the same or similar subpopulations or that the mechanisms regulating the activities of these cells are similar. Furthermore, our findings suggest that IF treatment can improve the discriminatory power of the ADCC assay in detecting transfusion or transplantation related immunity.

Full text

PDF
183

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg K., Heron I., Hamilton R. Purification of human interferon by antibody affinity chromatography, using highly absorbed anti-interferon. Scand J Immunol. 1978;8(5):429–436. doi: 10.1111/j.1365-3083.1978.tb00538.x. [DOI] [PubMed] [Google Scholar]
  2. Droller M. J., Borg H., Perlmann P. In vitro enhancement of natural and antibody-dependent lymphocyte-mediated cytotoxicity against tumor target cells by interferon. Cell Immunol. 1979 Oct;47(2):248–260. doi: 10.1016/0008-8749(79)90335-6. [DOI] [PubMed] [Google Scholar]
  3. Dumble L. J., MacDonald I. M., Kincaid-Smith P. Human renal allograft rejection is predicted by serial determinations of antibody-dependent cellular cytotoxicity. Transplantation. 1980;29(1):30–34. doi: 10.1097/00007890-198001000-00007. [DOI] [PubMed] [Google Scholar]
  4. Einhorn S., Blomgren H., Strander H. Interferon and spontaneous cytotoxicity in man. I. Enhancement of the spontaneous cytotoxicity of peripheral lymphocytes by human leukocyte interferon. Int J Cancer. 1978 Oct 15;22(4):405–412. doi: 10.1002/ijc.2910220407. [DOI] [PubMed] [Google Scholar]
  5. Herberman R. R., Ortaldo J. R., Bonnard G. D. Augmentation by interferon of human natural and antibody-dependent cell-mediated cytotoxicity. Nature. 1979 Jan 18;277(5693):221–223. doi: 10.1038/277221a0. [DOI] [PubMed] [Google Scholar]
  6. Heron I., Hokland M., Møller-Larsen A., Berg K. The effect of interferon on lymphocyte-mediated effector cell functions: selective enhancement of natural killer cells. Cell Immunol. 1979 Jan;42(1):183–187. doi: 10.1016/0008-8749(79)90232-6. [DOI] [PubMed] [Google Scholar]
  7. Kimber I., Moore M. Selective enhancement of human mononuclear leucocyte cytotoxic function by interferon. Scand J Immunol. 1981;13(4):375–381. doi: 10.1111/j.1365-3083.1981.tb00147.x. [DOI] [PubMed] [Google Scholar]
  8. Moore M., Potter M. R. Enhancement of human natural cell-mediated cytotoxicity by interferon. Br J Cancer. 1980 Mar;41(3):378–387. doi: 10.1038/bjc.1980.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ortaldo J. R., Pestka S., Slease R. B., Rubinstein M., Herberman R. B. Augmentation of human K-cell activity with interferon. Scand J Immunol. 1980;12(5):365–369. doi: 10.1111/j.1365-3083.1980.tb00079.x. [DOI] [PubMed] [Google Scholar]
  10. Parkman R., Rosen F. S., Rappeport J., Camitta B., Levey R. L., Nathan D. G. Detection of genetically determined histocompatibility antigen differences between HL-A identical and MLC nonreactive siblings. Transplantation. 1976 Feb;21(2):110–116. doi: 10.1097/00007890-197602000-00004. [DOI] [PubMed] [Google Scholar]
  11. Trinchieri G., Santoli D. Anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. Enhancement of human natural killer cell activity by interferon and antagonistic inhibition of susceptibility of target cells to lysis. J Exp Med. 1978 May 1;147(5):1314–1333. doi: 10.1084/jem.147.5.1314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Warren R. P., Storb R., Weiden P. L., Mickelson E. M., Thomas E. D. Direct and antibody-dependent cell-mediated cytotoxicity against HLA identical sibling lymphocytes. Correlation with marrow graft rejections. Transplantation. 1976 Dec;22(6):631–635. [PubMed] [Google Scholar]
  13. Warren R. P., Storb R., Weiden P. L., Su P. J., Thomas E. D. Prediction of marrow graft rejection by the lymphocyte-mediated cytotoxicity and antibody-dependent cell-mediated cytotoxicity assays. Transplantation. 1980 Aug;30(2):118–121. doi: 10.1097/00007890-198008000-00008. [DOI] [PubMed] [Google Scholar]
  14. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES