Skip to main content
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1980 Apr;40(1):136–146.

Cell-mediated immune responses between HLA-identical siblings: recognition of antigenic changes associated with acute myelogenous leukaemia.

K S Zier, C Huber, E Albert, H Braunsteiner
PMCID: PMC1536941  PMID: 6446429

Abstract

Cell-mediated immune reactions between a patient suffering from acute myelogenous leukaemia (AML) and an HLA-identical sibling were studied in order to characterize the in vitro reactions in MLC and CML prior to bone marrow transplantation. Our results indicated that antigenic differences were detectable between the blasts and the remission lymphocytes. While the normal sibling did not respond in MLC to her HLA-identical sister's remission lymphocytes, there was an anti-blast response. This proliferative response, however, did not lead to the development of detectable cytotoxic cells capable of destroying blast cells. Unrelated individuals, on the other hand, responded strongly both in MLC and CML to the allogeneic tumour blasts and remission lymphocytes of the patient and the lymphocytes of the healthy sibling. The kinetics and magnitude of the MLC response to blast cells was different from that to remission lymphocytes. This response indicated that the blast cells expressed antigenic differences which were recognized in MLC by both the HLA-identical sibling and unrelated individuals. Furthermore, these tumour cells were capable of sensitizing allogeneic, but not syngeneic lymphocytes to become cytotoxic, though they seemed to be more resistant to destruction in CML than normal cells.

Full text

PDF
136

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach F. H., Rood J. J. The major histocompatibility complex--genetics and biology (second of three parts). N Engl J Med. 1976 Oct 14;295(16):872–878. doi: 10.1056/NEJM197610142951606. [DOI] [PubMed] [Google Scholar]
  2. Bach F. H., Segall M., Zier K. S., Sondel P. M., Alter B. J., Bach M. L. Cell mediated immunity: separation of cells involved in recognitive and destructive phases. Science. 1973 Apr 27;180(4084):403–406. doi: 10.1126/science.180.4084.403. [DOI] [PubMed] [Google Scholar]
  3. Bach M. L., Bach F. H., Joo P. Leukemia-associated antigens in the mixed leukocyte culture test. Science. 1969 Dec 19;166(3912):1520–1522. doi: 10.1126/science.166.3912.1520. [DOI] [PubMed] [Google Scholar]
  4. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  5. Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fridman W. H., Kourilsky F. M. Stimulation of lymphocytes by autologous leukaemic cells in acute leukaemia. Nature. 1969 Oct 18;224(5216):277–279. doi: 10.1038/224277a0. [DOI] [PubMed] [Google Scholar]
  7. Greaves M. F., Brown G., Rapson N. T., Lister T. A. Antisera to acute lymphoblastic leukemia cells. Clin Immunol Immunopathol. 1975 May;4(1):67–84. doi: 10.1016/0090-1229(75)90041-0. [DOI] [PubMed] [Google Scholar]
  8. Kuntz M. M., Innes J. B., Weksler M. E. Lymphocyte transformation induced by autologous cells. IV. Human T-lymphocyte proliferation induced by autologous or allogeneic non-T lymphocytes. J Exp Med. 1976 May 1;143(5):1042–1054. doi: 10.1084/jem.143.5.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lee S. K., Oliver R. T. Autologous leukemia-specific T-cell-mediated lymphocytotoxicity in patients with acute myelogenous leukemia. J Exp Med. 1978 Mar 1;147(3):912–922. doi: 10.1084/jem.147.3.912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Oettgen H. F. Immunotherapy of cancer. N Engl J Med. 1977 Sep 1;297(9):484–491. doi: 10.1056/NEJM197709012970907. [DOI] [PubMed] [Google Scholar]
  11. Opelz G., Kiuchi M., Takasugi M., Terasaki P. I. Autologous stimulation of human lymphocyte subpopulation. J Exp Med. 1975 Nov 1;142(5):1327–1333. doi: 10.1084/jem.142.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reinsmoen N., Kersey J., Yunis E. J. Leukemia-associated antigens detected in primed lymphocyte tests. Transplant Proc. 1977 Mar;9(1 Suppl 1):199–201. [PubMed] [Google Scholar]
  13. Schendel D. J., Alter B. J., Bach F. H. The involvement of LD- and SD-region differences in MLC and CML: a three-cell experiment. Transplant Proc. 1973 Dec;5(4):1651–1655. [PubMed] [Google Scholar]
  14. Schendel D. J., Wank R., Dupont B. Standardization of the human in vitro cell-mediated lympholysis technique. Tissue Antigens. 1979 Feb;13(2):112–120. doi: 10.1111/j.1399-0039.1979.tb01146.x. [DOI] [PubMed] [Google Scholar]
  15. Schendel D. J., Wank R., Hansen J. A., Dupont B. Cell-mediated lympholysis in man: identification of a common target determinant associated with HLA-B12 and Bw35. Transplant Proc. 1977 Dec;9(4):1777–1780. [PubMed] [Google Scholar]
  16. Sondel P. M., O'Brien C., Porter L., Schlossman S. F., Chess L. Cell-mediated destruction of human leukemic cells by MHC identical lymphocytes: requirement for a proliferative trigger in vitro. J Immunol. 1976 Dec;117(6):2197–2203. [PubMed] [Google Scholar]
  17. TERASAKI P. I., MCCLELLAND J. D. MICRODROPLET ASSAY OF HUMAN SERUM CYTOTOXINS. Nature. 1964 Dec 5;204:998–1000. doi: 10.1038/204998b0. [DOI] [PubMed] [Google Scholar]
  18. Yunis E. J., Amos D. B. Three closely linked genetic systems relevant to transplantation. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3031–3035. doi: 10.1073/pnas.68.12.3031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zarling J. M., Raich P. C., McKeough M., Bach F. H. Generation of cytotoxic lymphocytes in vitro against autologous human leukaemia cells. Nature. 1976 Aug 19;262(5570):691–693. doi: 10.1038/262691a0. [DOI] [PubMed] [Google Scholar]
  20. Zier K. S., Huber C., Braunsteiner H. Restimulation in secondary MLC by autologous non-T cells. Scand J Immunol. 1979;9(6):493–498. doi: 10.1111/j.1365-3083.1979.tb03276.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES