Abstract
A micromethod for the study of specific antibody-dependent cellular cytotoxicity (ADCC) in human malaria is described, using cultured, asexual Plasmodium falciparum parasites as viable target cells. Lymphocytes from children with acute malaria, uninfected immune adult Gambians and adult Gambians infected with P. falciparum were capable of killing P. falciparum in vitro in the presence of malaria antibody. A parasite growth-promoting factor, produced by lymphocytes in non-immune serum and at a lymphocyte--parasite ratio of 10:1, in immune serum, was found to produce three-fold increases in growth of P. falciparum. The mechanisms by which ADCC may occur are also discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- COHEN S., McGREGOR I. A., CARRINGTON S. Gamma-globulin and acquired immunity to human malaria. Nature. 1961 Nov 25;192:733–737. doi: 10.1038/192733a0. [DOI] [PubMed] [Google Scholar]
- Coleman R. M., Rencricca N. J., Stout J. P., Brissette W. H., Smith D. M. Splenic mediated erythrocyte cytotoxicity in malaria. Immunology. 1975 Jul;29(1):49–54. [PMC free article] [PubMed] [Google Scholar]
- Cottrell B. J., Playfair J. H., De Souza B. J. Cell-mediated immunity in mice vaccinated against malaria. Clin Exp Immunol. 1978 Nov;34(2):147–158. [PMC free article] [PubMed] [Google Scholar]
- Greenwood B. M., Oduloju A. J., Stratton D. Lymphocyte changes in acute malaria. Trans R Soc Trop Med Hyg. 1977;71(5):408–410. doi: 10.1016/0035-9203(77)90039-6. [DOI] [PubMed] [Google Scholar]
- Jensen J. B., Trager W. Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method. J Parasitol. 1977 Oct;63(5):883–886. [PubMed] [Google Scholar]
- McDonald V., Phillips R. S. Increase in non-specific antibody mediated cytotoxicity in malarious mice. Clin Exp Immunol. 1978 Nov;34(2):159–163. [PMC free article] [PubMed] [Google Scholar]
- McGregor I. A., Williams K. Value of the gel-precipitation test in monitoring the endemicity of malaria in a rural African village. Isr J Med Sci. 1978 Jun;14(6):697–706. [PubMed] [Google Scholar]
- Phillips R. S., Jones V. E. Immunity to Plasmodium berghei in rats: maximum levels of protective antibody activity are associated with eradication of the infection. Parasitology. 1972 Feb;64(1):117–127. doi: 10.1017/s0031182000044693. [DOI] [PubMed] [Google Scholar]
- Phillips R. S. Plasmodium berghei: passive transfer of immunity by antisera and cells. Exp Parasitol. 1970 Jun;27(3):479–495. doi: 10.1016/0014-4894(70)90052-4. [DOI] [PubMed] [Google Scholar]
- Phillips R. S., Wolstencroft R. A., Brown I. N., Brown K. N., Dumonde D. C. Immunity to malaria. 3. Possible occurrence of a cell-mediated immunity to Plasmodium knowlesi in chronically infected and Freund's complete adjuvant-sensitized monkeys. Exp Parasitol. 1970 Oct;28(2):339–355. doi: 10.1016/0014-4894(70)90103-7. [DOI] [PubMed] [Google Scholar]
- Shen L., Lydyard P. M., Penfold P., Roitt I. M. Evidence for antibody-dependent cell-mediated cytotoxicity by T cells bearing receptors for IgG. Clin Exp Immunol. 1979 Feb;35(2):276–285. [PMC free article] [PubMed] [Google Scholar]
- Smalley M. E. Plasmodium falciparum gametocytogenesis in vitro. Nature. 1976 Nov 18;264(5583):271–272. doi: 10.1038/264271a0. [DOI] [PubMed] [Google Scholar]
- Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. doi: 10.1126/science.781840. [DOI] [PubMed] [Google Scholar]
- Weinbaum F. I., Evans C. B., Tigelaar R. E. Immunity to Plasmodium Berghei yoelii in mice. I. The course of infection in T cell and B cell deficient mice. J Immunol. 1976 Nov;117(5 PT2):1999–2005. [PubMed] [Google Scholar]