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. 1981 Mar;43(3):450–457.

Ontogeny of humoral immune function in normal chickens: a comparison of immunoglobulin-secreting cells in bone marrow, spleen, lungs and intestine.

E C Lawrence, F Arnaud-Battandier, J Grayson, I R Koski, N J Dooley, A V Muchmore, R M Blaese
PMCID: PMC1537196  PMID: 7026091

Abstract

A reverse haemolytic plaque was employed to study the ontogeny of immunoglobulin (Ig) secreting cells of either IgG, IgA, or IgM class in normal chickens. After hatching, IgM-secreting cells were detectable in the spleen by 3 days of age whereas IgG- and IgA-secreting cells were first noted at 6 days. Adult levels of Ig-secreting cells of all three classes were attained by 31 days of age in bone marrow and two separate lymphoid populations (lamina propria and intraepithelial lymphocytes). By contrast, adult levels of Ig-secreting cells were not obtained in either the spleen or the lungs until after 50 days of age. In the case of the spleen, the delay in attainment of adult levels of total Ig-secreting cells reflected the smaller spleen size in immature birds, whereas the percentages of cells secreting Ig of each class were in the adult range by 31 days. By contrast, the numbers of cells recovered from the lungs of 50-day-old chickens were near the adult range, while the percentages of cells secreting either IgG, IgA, or IgM were much fewer than those seen in the lungs of adult chickens. These data indicate that the lungs of normal chickens are populated more slowly with Ig-secreting cells than either the bone marrow, spleen, or intestine. At all ages studied, greater numbers of Ig-secreting cells, particularly of the IgG and IgM classes, were recovered from the bone marrow and spleen as compared to the lungs and intestine. Since only a portion of the total bone marrow population was studied, these data include that the bone marrow may be a major site of Ig-secreting cells in chickens beginning shortly after hatching.

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Selected References

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  1. ASKONAS B. A., HUMPHREY J. H. Formation of specific antibodies and gamma-globulin in vitro; a study of the synthetic ability of various tissues from rabbits immunized by different methods. Biochem J. 1958 Feb;68(2):252–261. doi: 10.1042/bj0680252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnaud-Battandier F., Lawrence E. C., Blaese R. M. Lymphoid populations of gut mucosa in chickens. Dig Dis Sci. 1980 Apr;25(4):252–259. doi: 10.1007/BF01308514. [DOI] [PubMed] [Google Scholar]
  3. Bienenstock J., Johnston N., Perey D. Y. Bronchial lymphoid tissue. I. Morphologic characteristics. Lab Invest. 1973 Jun;28(6):686–692. [PubMed] [Google Scholar]
  4. Blaese R. M., Muchmore A. V., Koski I., Dooley N. J. Infectious agammaglobulinemia: suppressor T cells with specificity for individual immunoglobulin classes. Adv Exp Med Biol. 1977;88:155–159. doi: 10.1007/978-1-4613-4169-7_14. [DOI] [PubMed] [Google Scholar]
  5. Blaese R. M., Weiden P. L., Koski I., Dooley N. Infectious agammaglobulinemia: transmission of immunodeficiency with grafts of agammaglobulinemic cells. J Exp Med. 1974 Oct 1;140(4):1097–1101. doi: 10.1084/jem.140.4.1097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cooper M. D., Cain W. A., Van Alten P. J., Good R. A. Development and function of the immunoglobulin producing system. I. Effect of bursectomy at different stages of development on germinal centers, plasma cells, immunoglobulins and antibody production. Int Arch Allergy Appl Immunol. 1969;35(3):242–252. [PubMed] [Google Scholar]
  7. Cooper M. D., Raymond D. A., Peterson R. D., South M. A., Good R. A. The functions of the thymus system and the bursa system in the chicken. J Exp Med. 1966 Jan 1;123(1):75–102. doi: 10.1084/jem.123.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Crabbé P. A., Heremans J. F. Lack of gamma A-immunoglobulin in serum of patients with steatorrhoea. Gut. 1966 Apr;7(2):119–127. doi: 10.1136/gut.7.2.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Crabbé P. A., Heremans J. F. The distribution of immunoglobulin-containing cells along the human gastrointestinal tract. Gastroenterology. 1966 Sep;51(3):305–316. [PubMed] [Google Scholar]
  10. Emery J. L., Dinsdale F. The postnatal development of lymphoreticular aggregates and lymph nodes in infants' lungs. J Clin Pathol. 1973 Jul;26(7):539–545. doi: 10.1136/jcp.26.7.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gathings W. E., Lawton A. R., Cooper M. D. Immunofluorescent studies of the development of pre-B cells, B lymphocytes and immunoglobulin isotype diversity in humans. Eur J Immunol. 1977 Nov;7(11):804–810. doi: 10.1002/eji.1830071112. [DOI] [PubMed] [Google Scholar]
  12. Gronowicz E., Coutinho A., Melchers F. A plaque assay for all cells secreting Ig of a given type or class. Eur J Immunol. 1976 Aug;6(8):588–590. doi: 10.1002/eji.1830060812. [DOI] [PubMed] [Google Scholar]
  13. HUMPHREY J. H., SULITZEANU B. D. The use of [14C] amino acids to study sites and rates of antibody synthesis in living hyperimmune rabbits. Biochem J. 1958 Jan;68(1):146–161. doi: 10.1042/bj0680146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hand W. L., Cantey J. R. Antibacterial mechanisms of the lower respiratory tract. I. Immunoglobulin synthesis and secretion. J Clin Invest. 1974 Feb;53(2):354–362. doi: 10.1172/JCI107567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kaltreider H. B., Kyselka L., Salmon S. E. Immunology of the lower respiratory tract. II. The plaque-forming response of canine lymphoid tissues to sheep erythrocytes after intrapulmonary or intravenous immunization. J Clin Invest. 1974 Aug;54(2):263–270. doi: 10.1172/JCI107761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kaltreider H. B., Turner F. N. Appearance of antibody-forming cells in lymphocytes from the lower respiratory tract of the dog after intrapulmonary or intravenous immunization with sheep erythrocytes. Am Rev Respir Dis. 1976 May;113(5):613–617. doi: 10.1164/arrd.1976.113.5.613. [DOI] [PubMed] [Google Scholar]
  17. Kincade P. W., Cooper M. D. Development and distribution of immunoglobulin-containing cells in the chicken. An immunofluorescent analysis using purified antibodies to mu, gamma and light chains. J Immunol. 1971 Feb;106(2):371–382. [PubMed] [Google Scholar]
  18. Lawrence E. C., Arnaud-Battandier F., Koski I. R., Dooley N. J., Muchmore A. V., Blaese R. M. Tissue distribution of immunoglobulin-secreting cells in normal and IgA-deficient chickens. J Immunol. 1979 Oct;123(4):1767–1771. [PubMed] [Google Scholar]
  19. Lawrence E. C., Blaese R. M., Martin R. R., Stevens P. M. Immunoglobulin secreting cells in normal human bronchial lavage fluids. J Clin Invest. 1978 Oct;62(4):832–835. doi: 10.1172/JCI109195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Milne R. W., Bienenstodk J., Perey D. Y. The influence of antigenic stimulation on the ontegeny of lymphoid aggregates and immunoglobulin-containing cells in mouse bronchial and intestinal mucosa. J Reticuloendothel Soc. 1975 Jun;17(6):361–369. [PubMed] [Google Scholar]
  21. Palladino M. A., Lerman S. P., Thorbecke G. J. Transfer of hypogammaglobulinemia in two inbred chicken strains by spleen cells from bursectomized donors. J Immunol. 1976 Jun;116(6):1673–1676. [PubMed] [Google Scholar]

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