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. 1981 Jul;45(1):1–8.

Evaluation of T cell subsets in myasthenia gravis using anti-T cell monoclonal antibodies.

S Berrih, C Gaud, M A Bach, H Le Brigand, J P Binet, J F Bach
PMCID: PMC1537262  PMID: 6458432

Abstract

Functional T cell subsets have been evaluated in the peripheral blood of patients with myasthenia gravis using monoclonal anti-T cell antibodies and a suppressor cell assay based on the suppression of the mixed-lymphocyte reaction by concanavalin A-activated lymphocytes. A significant decline of suppressor cells was found in a large proportion of patients, both by direct count using the anti-suppressor-cytotoxic T cell antibody (OKT8) and by the suppressor assay. Patients also showed an increase in immature T cells defined by their simultaneous reaction with the anti-helper cell (OKT4) and anti-suppressor cell (OKT8) antibody. Thymectomy tended to enhance the deficit in suppressor cells, and to induce the disappearance of double-labelled cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdou N. I., Lisak R. P., Zweiman B., Abrahamsohn I., Penn A. S. The thymus in myasthenia gravis. Evidence for altered cell populations. N Engl J Med. 1974 Dec 12;291(24):1271–1275. doi: 10.1056/NEJM197412122912403. [DOI] [PubMed] [Google Scholar]
  2. Bach J. F. Evaluation of T-cells and thymic serum factors in man using the rosette technique. Transplant Rev. 1973;16(0):196–217. doi: 10.1111/j.1600-065x.1973.tb00121.x. [DOI] [PubMed] [Google Scholar]
  3. Birnbaum G., Tsairis P. Thymic lymphocytes in myasthenia gravis. Ann Neurol. 1977 Apr;1(4):331–333. doi: 10.1002/ana.410010404. [DOI] [PubMed] [Google Scholar]
  4. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Csapo A. I., Pohanka O., Kaihola H. L. Progesterone deficiency and premature labour. Br Med J. 1974 Jan 26;1(5899):137–140. doi: 10.1136/bmj.1.5899.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Drachman D. B., Angus C. W., Adams R. N., Kao I. Effect of myasthenic patients' immunoglobulin on acetylcholine receptor turnover: selectivity of degradation process. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3422–3426. doi: 10.1073/pnas.75.7.3422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fritze D., Herrman C., Jr, Naeim F., Smith G. S., Walford R. L. HL-A antigens in myasthenia gravis. Lancet. 1974 Feb 16;1(7851):240–242. doi: 10.1016/s0140-6736(74)92548-3. [DOI] [PubMed] [Google Scholar]
  8. Galili U., Schlesinger M. The formation of stable E rosettes after neuraminidase treatment of either human peripheral blood lymphocytes or of sheep red blood cells. J Immunol. 1974 May;112(5):1628–1634. [PubMed] [Google Scholar]
  9. Goldstein G., Manganaro A. Thymin: a thymic polypeptide causing the neuromuscular block of myasthenia gravis. Ann N Y Acad Sci. 1971 Sep 15;183:230–240. doi: 10.1111/j.1749-6632.1971.tb30754.x. [DOI] [PubMed] [Google Scholar]
  10. Huang S. W., Rose J. W., Mayer R. F. Assessment of cellular and humoral immunity of myasthenics. J Neurol Neurosurg Psychiatry. 1977 Nov;40(11):1053–1059. doi: 10.1136/jnnp.40.11.1053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Itoyama Y., Kawanami S., Goto I., Kuroiwa Y. T and B lymphocytes in myasthenia gravis. Clin Exp Immunol. 1979 Sep;37(3):452–456. [PMC free article] [PubMed] [Google Scholar]
  12. Kerbel R. S., Eidinger D. Enhanced immune responsiveness to a thymus-independent antigen early after adult thymectomy: evidence for short-lived inhibitory thymus-derived cells. Eur J Immunol. 1972 Apr;2(2):114–118. doi: 10.1002/eji.1830020204. [DOI] [PubMed] [Google Scholar]
  13. Kung P., Goldstein G., Reinherz E. L., Schlossman S. F. Monoclonal antibodies defining distinctive human T cell surface antigens. Science. 1979 Oct 19;206(4416):347–349. doi: 10.1126/science.314668. [DOI] [PubMed] [Google Scholar]
  14. Le Brigand H., Leuasseur P., Merlier M., Rojas-Miranda A., Gaud C., Noviant Y. Cent thymectomies chez des myasthéniques. Résultats lointains. Rev Neurol (Paris) 1972 Apr;126(4):267–274. [PubMed] [Google Scholar]
  15. Opelz G., Keesey J., Glovsky M. M., Gale R. P. Autoreactivity between lymphocytes and thymus cells in myasthenia gravis. Arch Neurol. 1978 Jul;35(7):413–415. doi: 10.1001/archneur.1978.00500310015003. [DOI] [PubMed] [Google Scholar]
  16. Papatestas A. E., Alpert L. I., Osserman K. E., Osserman R. S., Kark A. E. Studies in myasthenia gravis: effects of thymectomy. Results on 185 patients with nonthymomatous and thymomatous myasthenia gravis, 1941-1969. Am J Med. 1971 Apr;50(4):465–474. doi: 10.1016/0002-9343(71)90336-6. [DOI] [PubMed] [Google Scholar]
  17. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2. J Immunol. 1980 Mar;124(3):1301–1307. [PubMed] [Google Scholar]
  18. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells. J Immunol. 1979 Sep;123(3):1312–1317. [PubMed] [Google Scholar]
  19. Reinherz E. L., Schlossman S. F. The differentiation and function of human T lymphocytes. Cell. 1980 Apr;19(4):821–827. doi: 10.1016/0092-8674(80)90072-0. [DOI] [PubMed] [Google Scholar]
  20. Richman D. P., Patrick J., Arnason B. G. Cellular immunity in myasthenia gravis. Response to purified acetylcholine receptor and autologous thymocytes. N Engl J Med. 1976 Mar 25;294(13):694–698. doi: 10.1056/NEJM197603252941304. [DOI] [PubMed] [Google Scholar]
  21. Rotter V., Trainin N. Thymus cell population exerting a regulatory function in the immune response of mice to polyvinyl pyrrolidone. Cell Immunol. 1974 Jul;13(1):76–86. doi: 10.1016/0008-8749(74)90228-7. [DOI] [PubMed] [Google Scholar]
  22. Sakane T., Green I. Human suppressor T cells induced by concanavalin A: suppressor T cells belong to distinctive T cell subclasses. J Immunol. 1977 Sep;119(3):1169–1178. [PubMed] [Google Scholar]
  23. Stanley E. F., Drachman D. B. Effect of myasthenic immunoglobulin on acetylcholine receptors of intact mammalian neuromuscular junctions. Science. 1978 Jun 16;200(4347):1285–1287. doi: 10.1126/science.663610. [DOI] [PubMed] [Google Scholar]
  24. Terasaki P. I., Mickey M. R. HL-A haplotypes of 32 diseases. Transplant Rev. 1975;22:105–119. doi: 10.1111/j.1600-065x.1975.tb01553.x. [DOI] [PubMed] [Google Scholar]
  25. Wijermans P., Oosterhuis H. J., Astaldi G. C., Schellekens P. T., Astaldi A. Influence of adult thymectomy on immunocompetence in patients with myasthenia gravis. J Immunol. 1980 Apr;124(4):1977–1982. [PubMed] [Google Scholar]
  26. Zilko P. J., Dawkins R. L., Holmes K., Witt C. Genetic control of suppressor lymphocyte function in myasthenia gravis: relationship of impaired suppressor function to HLA-B8/DRW3 and cold reactive lymphocytotoxic antibodies. Clin Immunol Immunopathol. 1979 Oct;14(2):222–230. doi: 10.1016/0090-1229(79)90143-0. [DOI] [PubMed] [Google Scholar]

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