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. 1981 Feb;43(2):417–424.

A radioimmunoassay for serum and gingival crevicular fluid antibodies to a purified protein of Streptococcus mutans.

R Smith, T Lehner
PMCID: PMC1537267  PMID: 7023762

Abstract

A solid-phase radioimmunoassay was developed to measure serum IgG antibodies to a purified protein antigen I/II prepared from Streptococcus mutans. The assay was specific to this antigen and significant binding of 125I-radiolabelled antiserum was found only in sera from rhesus monkeys immunized with the antigen I/II but not in sham-immunized monkeys or those immunized with streptococcal antigen III. A very significant correlation was found in serum IgG antibodies tested by the radioimmunoassay and an immunofluorescent technique (r = 0.88, P less than 0.001). The sensitivity of the double-layer radioimmunoassay was increased 10 times by the addition of a third antibody layer and this enabled gingival crevicular fluid antibodies to be measured. Comparison of paired samples of serum and crevicular fluid revealed a very significant correlation between IgG antibodies to streptococcal antigen I/II in the the two fluids (P less than 0.001). These findings suggest that serum antibodies can reach the tooth surface via gingival crevicular fluid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Catt K., Tregear G. W. Solid-phase radioimmunoassay in antibody-coated tubes. Science. 1967 Dec 22;158(3808):1570–1572. doi: 10.1126/science.158.3808.1570. [DOI] [PubMed] [Google Scholar]
  2. Challacombe S. J., Guggenheim B., Lehner T. Antibodies to an extract of Streptococcus mutans, containing glucosyltransferase activity, related to dental caries in man. Arch Oral Biol. 1973 Jun;18(6):657–668. doi: 10.1016/0003-9969(73)90001-0. [DOI] [PubMed] [Google Scholar]
  3. Challacombe S. J., Lehner T. Serum and salivary antibodies to cariogenic bacteria in man. J Dent Res. 1976 Apr;55(Spec No):C139–C148. doi: 10.1177/002203457605500306011. [DOI] [PubMed] [Google Scholar]
  4. Challacombe S. J., Russell M. W., Hawkes J. E., Bergmeier L. A., Lehner T. Passage of immunoglobulins from plasma to the oral cavity in rhesus monkeys. Immunology. 1978 Dec;35(6):923–931. [PMC free article] [PubMed] [Google Scholar]
  5. Evans R. T., Genco R. J. Inhibition of glucosyltransferase activity by antisera to known serotypes of Streptococcus mutans. Infect Immun. 1973 Feb;7(2):237–241. doi: 10.1128/iai.7.2.237-241.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  7. Hay F. C., Nineham L. J., Roitt I. M. Routine assay for detection of IgG and IgM antiglobulins in seronegative and seropositive rheumatoid arthritis. Br Med J. 1975 Jul 26;3(5977):203–204. doi: 10.1136/bmj.3.5977.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayashi J. A., Shklair I. L., Bahn A. N. Immunization with dextransucrases and glycosidic hydrolases. J Dent Res. 1972 Mar-Apr;51(2):436–442. doi: 10.1177/00220345720510023201. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lamb J. R., Kontiainen S., Lehner T. A comparative investigation of the generation of specific T cell-helper function induced by Streptococcus mutans in monkeys and mice. J Immunol. 1980 May;124(5):2384–2389. [PubMed] [Google Scholar]
  11. Lamb J. R., Kontiainen S., Lehner T. Generation of specfic T-cell suppressor function induced by Streptococcus mutans in monkeys and mice. Infect Immun. 1979 Dec;26(3):903–909. doi: 10.1128/iai.26.3.903-909.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lehner T., Challacombe S. J., Wilton J. M., Caldwell J. Cellular and humoral immune responses in vaccination against dental caries in monkeys. Nature. 1976 Nov 4;264(5581):69–72. doi: 10.1038/264069a0. [DOI] [PubMed] [Google Scholar]
  13. Lehner T., Murray J. J., Winter G. B., Caldwell J. Antibodies to Streptococcus mutans and immunoglobulin levels in children with dental caries. Arch Oral Biol. 1978;23(12):1061–1067. doi: 10.1016/0003-9969(78)90109-7. [DOI] [PubMed] [Google Scholar]
  14. Lehner T., Russell M. W., Caldwell J. Immunisation with a purified protein from Streptococcus mutans against dental caries in rhesus monkeys. Lancet. 1980 May 10;1(8176):995–996. doi: 10.1016/s0140-6736(80)91437-3. [DOI] [PubMed] [Google Scholar]
  15. McGhee, Michalek S. M., Webb J., Navia J. M., Rahman A. F., Legler D. W. Effective immunity to dental caries: protection of gnotobiotic rats by local immunization with Streptococcus mutans. J Immunol. 1975 Jan;114(1 Pt 2):300–305. [PubMed] [Google Scholar]
  16. Russell M. W., Bergmeier L. A., Zanders E. D., Lehner T. Protein antigens of Streptococcus mutans: purification and properties of a double antigen and its protease-resistant component. Infect Immun. 1980 May;28(2):486–493. doi: 10.1128/iai.28.2.486-493.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Russell M. W., Challacombe S. J., Lehner T. Serum glucosyltransferase-inhibiting antibodies and dental caries in rhesus monkeys immunized against Streptococcus mutans. Immunology. 1976 May;30(5):619–627. [PMC free article] [PubMed] [Google Scholar]
  18. Russell M. W., Challacombe S. J., Lehner T. Specificity of antibodies induced by Streptococcus mutans during immunization against dental caries. Immunology. 1980 May;40(1):97–106. [PMC free article] [PubMed] [Google Scholar]
  19. Russell M. W. Purification and properties of a protein surface antigen of Streptococcus mutants. Microbios. 1979;25(99):7–18. [PubMed] [Google Scholar]
  20. Scully C. M., Lehner T. Opsonization, phagocytosis and killing of Streptococcus mutans by polymorphonuclear leukocytes, in relation to dental caries in the rhesus monkey (Macaca mulatta). Arch Oral Biol. 1979;24(4):307–312. doi: 10.1016/0003-9969(79)90093-1. [DOI] [PubMed] [Google Scholar]
  21. Skapski H., Lehner T. A crevicular washing method for investigating immune components of crevicular fluid in man. J Periodontal Res. 1976 Feb;11(1):19–24. doi: 10.1111/j.1600-0765.1976.tb00046.x. [DOI] [PubMed] [Google Scholar]
  22. Sulphinpyrazone after myocardial infarction. Lancet. 1978 Mar 18;1(8064):593–594. [PubMed] [Google Scholar]

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