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. 1981 Sep;45(3):475–479.

Enumeration of T lymphocytes subsets in autoimmune disease using monoclonal antibodies.

F Raeman, W De Cock, T De Beukelaar, J De Cree, H Verhaegen
PMCID: PMC1537396  PMID: 6461446

Abstract

The numbers of T lymphocytes, B lymphocytes, helper and suppressor T lymphocytes were measured in peripheral blood of patients with autoimmune disease (rheumatoid arthritis, erythema nodosum, Sjögren's disease, Wegener's disease, idiopathic thrombocytopenia, pernicious anaemia and Hashimoto's disease). B lymphocytes were enumerated by direct immunofluorescence and T lymphocytes by E rosette tests and by indirect immunofluorescence with OKT3.PAN Helper and suppressor T lymphocytes were determined by indirect immunofluorescence with OKT4.IND and OKT8.SUP respectively. The numbers of T lymphocytes, B lymphocytes and helper T lymphocytes in patients with autoimmune disease were normal, but the numbers of suppressor T lymphocytes were significantly lower.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach M. A., Phan-Dinh-Tuy F., Tournier E., Chatenoud L., Bach J. F., Martin C., Degos J. D. Deficit of suppressor T cells in active multiple sclerosis. Lancet. 1980 Dec 6;2(8206):1221–1223. doi: 10.1016/s0140-6736(80)92480-0. [DOI] [PubMed] [Google Scholar]
  2. Bresnihan B., Jasin H. E. Suppressor function of peripheral blood mononuclear cells in normal individuals and in patients with systemic lupus erythematosus. J Clin Invest. 1977 Jan;59(1):106–116. doi: 10.1172/JCI108607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fauci A. S., Steinberg A. D., Haynes B. F., Whalen G. Immunoregulatory aberrations in systemic lupus erythematosus. J Immunol. 1978 Oct;121(4):1473–1479. [PubMed] [Google Scholar]
  4. Kaplan M. E., Clark C. An improved rosetting assay for detection of human T lymphocytes. J Immunol Methods. 1974 Jul;5(2):131–135. doi: 10.1016/0022-1759(74)90003-9. [DOI] [PubMed] [Google Scholar]
  5. Kaufman D. B., Bostwick E. Defective suppressor T-cell activity in systemic lupus erythematosus. Clin Immunol Immunopathol. 1979 May;13(1):9–18. doi: 10.1016/0090-1229(79)90015-1. [DOI] [PubMed] [Google Scholar]
  6. Krakauer R. S., Clough J. D., Frank S., Sundeen J. T. Suppressor cell function defect in idiopathic systemic lupus erythematosus. Clin Immunol Immunopathol. 1979 Nov;14(3):327–333. doi: 10.1016/0090-1229(79)90158-2. [DOI] [PubMed] [Google Scholar]
  7. Kung P., Goldstein G., Reinherz E. L., Schlossman S. F. Monoclonal antibodies defining distinctive human T cell surface antigens. Science. 1979 Oct 19;206(4416):347–349. doi: 10.1126/science.314668. [DOI] [PubMed] [Google Scholar]
  8. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  9. Morimoto C., Abe T., Homma M. Altered function of suppressor T lymphocytes in patients with active systemic lupus erythematosus--in vitro immune response to autoantigen. Clin Immunol Immunopathol. 1979 Jun;13(2):161–170. doi: 10.1016/0090-1229(79)90060-6. [DOI] [PubMed] [Google Scholar]
  10. Morimoto C. Loss of suppressor T-lymphocyte function in patients with systemic lupus erythematosus (SLE). Clin Exp Immunol. 1978 Apr;32(1):125–133. [PMC free article] [PubMed] [Google Scholar]
  11. Reinherz E. L., Kung P. C., Goldstein G., Levey R. H., Schlossman S. F. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1588–1592. doi: 10.1073/pnas.77.3.1588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. Further characterization of the human inducer T cell subset defined by monoclonal antibody. J Immunol. 1979 Dec;123(6):2894–2896. [PubMed] [Google Scholar]
  13. Reinherz E. L., Rubinstein A., Geha R. S., Strelkauskas A. J., Rosen F. S., Schlossman S. F. Abnormalities of immunoregulatory T cells in disorders of immune function. N Engl J Med. 1979 Nov 8;301(19):1018–1022. doi: 10.1056/NEJM197911083011902. [DOI] [PubMed] [Google Scholar]
  14. Reinherz E. L., Schlossman S. F. Current concepts in immunology: Regulation of the immune response--inducer and suppressor T-lymphocyte subsets in human beings. N Engl J Med. 1980 Aug 14;303(7):370–373. doi: 10.1056/NEJM198008143030704. [DOI] [PubMed] [Google Scholar]
  15. de Cock W., de Cree J., Verhaegen H. Enumeration of human peripheral T lymphocytes with E-rosettes and OKT3. PAN monoclonal antibody: a close correlation. Thymus. 1980 Dec;2(3):133–137. [PubMed] [Google Scholar]

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