Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1978 Oct;34(1):46–51.

The role of macrophages in the lymphoproliferative response to Mycobacterium leprae in vitro.

H Hirschberg
PMCID: PMC1537460  PMID: 373933

Abstract

Peripheral blood lymphocytes from patients suffering from lepromatous leprosy do not normally react in vitro to stimulation by Mycobacterium leprae antigens. In contrast, we found that T cells from non-responding patients in combination with macrophages from responding patients or healthy contacts did respond well to M. leprae. Conversely, T cells from responding patients or healthy contacts in combinations with macrophages from non-responding patients failed to respond. It seems, therefore, that the lack of response normally observed in in vitro tests using cells from lepromatous leprosy patients is due to a failure of their macrophages to present M. leprae antigens in an immunogenic form.

Full text

PDF
46

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergholtz B. O., Thorsby E. Macrophage-dependent response of immune human T lymphocytes to PPD in vitro. Influence of HLA-D histocompatibility. Scand J Immunol. 1977;6(8):779–786. doi: 10.1111/j.1365-3083.1977.tb02151.x. [DOI] [PubMed] [Google Scholar]
  2. Berlinger N. T., Lopez C., Good R. A. Facilitation or attenuation of mixed leukocyte culture responsiveness by adherent cells. Nature. 1976 Mar 11;260(5547):145–146. doi: 10.1038/260145a0. [DOI] [PubMed] [Google Scholar]
  3. Cline M. J., Swett V. C. The interaction of human monocytes and lymphocytes. J Exp Med. 1968 Dec 1;128(6):1309–1325. doi: 10.1084/jem.128.6.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Feldmann M. Are the Ir genes expressed by macrophages? Nature. 1977 May 12;267(5607):105–105. doi: 10.1038/267105a0. [DOI] [PubMed] [Google Scholar]
  5. Godal T., Lofgren M., Negassi K. Immune response to M. leprae of healthy leprosy contacts. Int J Lepr Other Mycobact Dis. 1972 Jul-Sep;40(3):243–250. [PubMed] [Google Scholar]
  6. Godal T., Rees R. J., Lamvik J. O. Lymphocyte-mediated modification of blood-derived macrophage function in vitro; inhibition of growth of intracellular mycobacteria with lymphokines. Clin Exp Immunol. 1971 Apr;8(4):625–637. [PMC free article] [PubMed] [Google Scholar]
  7. Hersh E. M., Harris J. E. Macrophage-lymphocyte interaction in the antigen-induced blastogenic response of human peripheral blood leukocytes. J Immunol. 1968 Jun;100(6):1184–1194. [PubMed] [Google Scholar]
  8. Hirschberg H., Kaakinen A., Thorsby E. Presence of HLA-D determinants on human macrophages. Nature. 1976 Sep 2;263(5572):63–64. doi: 10.1038/263063a0. [DOI] [PubMed] [Google Scholar]
  9. Lenzini L., Rottoli P., Rottoli L. The spectrum of human tuberculosis. Clin Exp Immunol. 1977 Feb;27(2):230–237. [PMC free article] [PubMed] [Google Scholar]
  10. Miller J. F., Vadas M. A., Whitelaw A., Gamble J., Bernard C. Histocompatibility linked immune responsiveness and restrictions imposed on sensitized lymphocytes. J Exp Med. 1977 Jun 1;145(6):1623–1628. doi: 10.1084/jem.145.6.1623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ridley D. S., Jopling W. H. Classification of leprosy according to immunity. A five-group system. Int J Lepr Other Mycobact Dis. 1966 Jul-Sep;34(3):255–273. [PubMed] [Google Scholar]
  12. Rosenthal A. S., Barcinski M. A., Blake J. T. Determinant selection is a macrophage dependent immune response gene function. Nature. 1977 May 12;267(5607):156–158. doi: 10.1038/267156a0. [DOI] [PubMed] [Google Scholar]
  13. Rosenthal A. S., Shevach E. M. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973 Nov 1;138(5):1194–1212. doi: 10.1084/jem.138.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schwartz R. H., David C. S., Sachs D. H., Paul W. E. T lymphocyte-enriched murine peritoneal exudate cells. III. Inhibition of antigen-induced T lymphocyte Proliferation with anti-Ia antisera. J Immunol. 1976 Aug;117(2):531–540. [PubMed] [Google Scholar]
  15. Shevach E. M., Rosenthal A. S. Function of macrophages in antigen recognition by guinea pig T lymphocytes. II. Role of the macrophage in the regulation of genetic control of the immune response. J Exp Med. 1973 Nov 1;138(5):1213–1229. doi: 10.1084/jem.138.5.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wisloff F., Froland S. S. Antibody-dependent lymphocyte-mediated cytotoxicity in man: no requirement for lymphocytes with membrane-bound immunoglobulin. Scand J Immunol. 1973;2(2):151–157. doi: 10.1111/j.1365-3083.1973.tb02026.x. [DOI] [PubMed] [Google Scholar]
  17. de Vries R. R., Fat R. F., Nijenhuis L. E., van Rood J. J. HLA-linked genetic control of host response to Mycobacterium leprae. Lancet. 1976 Dec 18;2(7999):1328–1330. doi: 10.1016/s0140-6736(76)91975-9. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES