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. 1978 Jul;33(1):57–64.

Cell-mediated immune responses in owl monkeys (Aotus trivirgatus) with trachoma to soluble antigens of Chlamydia trachomatis.

D L Sacks, W J Todd, A B Macdonald
PMCID: PMC1537521  PMID: 101327

Abstract

The first temporal study of the cell-mediated immune responses (CMI) following ocular infections with Chlamydia trachomatis is presented. We examined the CMI of owl monkeys infected with trachoma to soluble antigens of C. trachomatis by leucocyte migration inhibition (LIF) and delayed hypersensitivity skin testing. Delayed hypersensitivity of a systemic nature developed after a local eye infection in owl monkeys; clearance of inclusions from conjunctival cells coincided with the onset of this response. The association of eye secretion and circulating antibodies with recovery from primary infection was not so striking. Both cellular and humoral immune responses persisted for at least 2 months, at which time all test animals were completely resistant to re-infection. The elicitation of cell-mediated immune reactions with solubilized chlamydial antigens may permit the isolation of specific antigens involved in the generation of protective immunity in the owl monkey model.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEDSON S. P., BARWELL C. F. The laboratory diagnosis of lymphogranuloma venereum. J Clin Pathol. 1949 Nov;2(4):241–249. doi: 10.1136/jcp.2.4.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BENEDICT A. A., TIPS R. L., EDDY D. Antigenic studies on the psittacosis-lymphogranuloma group of viruses. I. Acid-soluble complement-fixing and skin test antigens. Tex Rep Biol Med. 1955;13(2):206–212. [PubMed] [Google Scholar]
  3. Barenfanger J., MacDonald A. B. The role of immunoglobulin in the neutralization of trachoma infectivity. J Immunol. 1974 Nov;113(5):1607–1617. [PubMed] [Google Scholar]
  4. Barwell C. F., Dunlop E. M., Race J. W. Results of complement-fixation and intradermal tests for Bedsoniae in genital infection, disease of the eye and Reiter's disease. Am J Ophthalmol. 1967 May;63(5 Suppl):1527–1534. doi: 10.1016/0002-9394(67)94142-6. [DOI] [PubMed] [Google Scholar]
  5. Bell S. D., Fraser C. E. Experimental trachoma in owl monkeys. Am J Trop Med Hyg. 1969 Jul;18(4):568–572. doi: 10.4269/ajtmh.1969.18.568. [DOI] [PubMed] [Google Scholar]
  6. Bendixen G., Soborg M. A leucocyte migration technique for in vitro detection of cellular (delayed type) hypersensitivity in man. Dan Med Bull. 1969 Jan;16(1):1–6. [PubMed] [Google Scholar]
  7. Collier L. H. The immunopathology of trachoma: some facts and fancies. Arch Gesamte Virusforsch. 1967;22(1):280–293. doi: 10.1007/BF01240523. [DOI] [PubMed] [Google Scholar]
  8. GRAYSTON J. T., WANG S. P., WOOLRIDGE R. L., YANG Y. F., JOHNSTON P. B. Trachoma-studies of etiology, laboratory diagnosis, and prevention. J Am Med Assoc. 1960 Apr 9;172:1577–1586. doi: 10.1001/jama.1960.03020150001001. [DOI] [PubMed] [Google Scholar]
  9. Ghione M., Werner G. H., Cerulli L. Problèmes immunologiques du trachome. Rev Int Trach. 1974 May;51(1):3–78. [PubMed] [Google Scholar]
  10. Grayston J. T., Wang S. New knowledge of chlamydiae and the diseases they cause. J Infect Dis. 1975 Jul;132(1):87–105. doi: 10.1093/infdis/132.1.87. [DOI] [PubMed] [Google Scholar]
  11. Mackaness G. B. Resistance to intracellular infection. J Infect Dis. 1971 Apr;123(4):439–445. doi: 10.1093/infdis/123.4.439. [DOI] [PubMed] [Google Scholar]
  12. McComb D. E., Nichols R. L. Antibodies to trachoma in eye secretions of Saudi Arab children. Am J Epidemiol. 1969 Oct;90(4):278–284. doi: 10.1093/oxfordjournals.aje.a121071. [DOI] [PubMed] [Google Scholar]
  13. McComb D. E., Puzniak C. I. Micro cell culture method for isolation of Chlamydia trachomatis. Appl Microbiol. 1974 Oct;28(4):727–729. doi: 10.1128/am.28.4.727-729.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NICHOLS R. L., McCOMB D. E. Immunofluorescent studies with trachoma and related antigens. J Immunol. 1962 Oct;89:545–554. [PubMed] [Google Scholar]
  15. Nichols R. L., Bobb A. A., Haddad N. A., McComb D. E. Immunofluorescent studies of the microbiologic epidemiology of trachoma in Saudi Arabia. Am J Ophthalmol. 1967 May;63(5 Suppl):1372–1408. doi: 10.1016/0002-9394(67)94123-2. [DOI] [PubMed] [Google Scholar]
  16. Orenstein N. S., Mull J. D., Thompson S. E., 3rd Immunity to chlamydial infections of the eye. V. Passive transfer of antitrachoma antibodies to owl monkeys. Infect Immun. 1973 Apr;7(4):600–603. doi: 10.1128/iai.7.4.600-603.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rocklin R. E. Products of activated lymphocytes: leukocyte inhibitory factor (LIF) distinct from migration inhibitory factor (MIF). J Immunol. 1974 Apr;112(4):1461–1466. [PubMed] [Google Scholar]
  18. Sowa S., Sowa J., Collier L. H., Blyth W. A. Trachoma vaccine field trials in The Gambia. J Hyg (Lond) 1969 Dec;67(4):699–717. doi: 10.1017/s0022172400042157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wang S. P., Grayston J. T. Pannus with experimental trachoma and inclusion conjunctivitis agent infection of Taiwan monkeys. Am J Ophthalmol. 1967 May;63(5 Suppl):1133–1145. doi: 10.1016/0002-9394(67)94095-0. [DOI] [PubMed] [Google Scholar]
  20. Watson R. R., MacDonald A. B., Murray E. S., Modabber F. Z. Immunity to chlamydial infections of the eye. 3. Presence and duration of delayed hypersensitivity to guinea pig inclusion conjuctivitis. J Immunol. 1973 Aug;111(2):618–623. [PubMed] [Google Scholar]
  21. Woolridge R. L., Grayston J. T., Perrin E. B., Yang C. Y., Cheng K. H., Chang I. H. Natural history of trachoma in Taiwan school children. Am J Ophthalmol. 1967 May;63(5 Suppl):1313–1320. doi: 10.1016/0002-9394(67)94118-9. [DOI] [PubMed] [Google Scholar]

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