Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1978 Dec;34(3):417–422.

Passage of intact IgG from plasma to the oral cavity via crevicular fluid

S J Challacombe, M W Russell, Jane Hawkes
PMCID: PMC1537551  PMID: 105828

Abstract

The aim of this investigation was to determine whether IgG could pass from the blood to the oral cavity. Pure IgG was prepared from monkey serum, by ion exchange chromatography and gel filtration, and was radiolabelled with 125I. This was injected intravenously into eight Rhesus monkeys. Radioactivity could be detected in crevicular fluid washings, and in mixed and parotid saliva samples 30 min after injection. Ultracentrifugation on sucrose density gradients revealed that most of the radioactivity in crevicular fluid washings was associated with proteins having a sedimentation coefficient similar to marker IgG. Radioactivity in parotid saliva was not found in the IgG zone, but was present in zones with sedimentation coefficients of approximately 4·5S and 1S. The results suggest that IgG passes as an intact molecule from plasma to crevicular fluid, and support the hypothesis that serum antibodies could play a role in protection against dental caries.

Full text

PDF
417

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bader H. I., Goldhaber P. The passage of intravenously administered tetracycline in the gingival sulcus of dogs. J Oral Ther Pharmacol. 1966 Mar;2(5):324–329. [PubMed] [Google Scholar]
  2. CARLSSON J., EGELBERG J. EFFECT OF DIET ON EARLY PLAQUE FORMATION IN MAN. Odontol Revy. 1965;16:112–125. [PubMed] [Google Scholar]
  3. Caldwell J., Challacombe S. J., Lehner T. A sequential bacteriological and serological investigation of Rhesus monkeys immunised against dental caries with Streptococcus mutans. J Med Microbiol. 1977 May;10(2):213–224. doi: 10.1099/00222615-10-2-213. [DOI] [PubMed] [Google Scholar]
  4. Challacombe S. J., Guggenheim B., Lehner T. Antibodies to an extract of Streptococcus mutans, containing glucosyltransferase activity, related to dental caries in man. Arch Oral Biol. 1973 Jun;18(6):657–668. doi: 10.1016/0003-9969(73)90001-0. [DOI] [PubMed] [Google Scholar]
  5. Challacombe S. J. Immunoglobulins in parotid saliva and serum in relation to dental caries in man. Caries Res. 1976;10(3):165–177. doi: 10.1159/000260199. [DOI] [PubMed] [Google Scholar]
  6. Challacombe S. J., Lehner T. Serum and salivary antibodies to cariogenic bacteria in man. J Dent Res. 1976 Apr;55(Spec No):C139–C148. doi: 10.1177/002203457605500306011. [DOI] [PubMed] [Google Scholar]
  7. Challacombe S. J. Serum complement-fixing antibodies in human dental caries. Caries Res. 1974;8(1):84–95. doi: 10.1159/000260095. [DOI] [PubMed] [Google Scholar]
  8. Evans R. T., Genco R. J. Inhibition of glucosyltransferase activity by antisera to known serotypes of Streptococcus mutans. Infect Immun. 1973 Feb;7(2):237–241. doi: 10.1128/iai.7.2.237-241.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Guggenheim B., Newbrun E. Extracellular glucosyltransferase activity of an HS strain of Streptococcus mutans. Helv Odontol Acta. 1969 Oct;13(2):84–97. [PubMed] [Google Scholar]
  10. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  11. Holmberg K., Killander J. Quantitative determination of immunoglobuline (IgG, IgA and IgM) and identification of IgA-type in the gingival fluid. J Periodontal Res. 1971;6(1):1–8. doi: 10.1111/j.1600-0765.1971.tb00581.x. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Lehner T., Challacombe S. J., Caldwell J. An immunological investigation into the prevention of caries in deciduous teeth of rhesus monkeys. Arch Oral Biol. 1975 May-Jun;20(5-6):305–310. doi: 10.1016/0003-9969(75)90019-9. [DOI] [PubMed] [Google Scholar]
  14. Lehner T., Challacombe S. J., Caldwell J. Immunologic basis for vaccination against dental caries in rhesus monkeys. J Dent Res. 1976 Apr;55(Spec No):C166–C180. doi: 10.1177/002203457605500311011. [DOI] [PubMed] [Google Scholar]
  15. Lehner T., Challacombe S. J., Caldwell J. Immunological and bacteriological basis for vaccination against dental caries in rhesus monkeys. Nature. 1975 Apr 10;254(5500):517–520. doi: 10.1038/254517a0. [DOI] [PubMed] [Google Scholar]
  16. Mason D. K., Harden R. M., Alexander W. D. The salivary and thyroid glands. A comparative study in man. Br Dent J. 1967 Jun 6;122(11):485–489. [PubMed] [Google Scholar]
  17. McGhee J. R., Michakel S. M., Navia J. M., Narkates A. J. Effective immunity to dental caries: studies of active and passive immunity to Streptococcus mutans in malnourished rats. J Dent Res. 1976 Apr;55(Spec No):C206–C214. doi: 10.1177/002203457605500314011. [DOI] [PubMed] [Google Scholar]
  18. Michalek S. M., McGhee J. R., Mestecky J., Arnold R. R., Bozzo L. Ingestion of Streptococcus mutans induces secretory immunoglobulin A and caries immunity. Science. 1976 Jun 18;192(4245):1238–1240. doi: 10.1126/science.1273589. [DOI] [PubMed] [Google Scholar]
  19. Olson G. A., Bleiweis A. S., Small P. A., Jr Adherence inhibition of Streptococcus mutans: an assay reflecting a possible role of antibody in dental caries prophylaxis. Infect Immun. 1972 Apr;5(4):419–427. doi: 10.1128/iai.5.4.419-427.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Russell M. W., Challacombe S. J., Lehner T. Serum glucosyltransferase-inhibiting antibodies and dental caries in rhesus monkeys immunized against Streptococcus mutans. Immunology. 1976 May;30(5):619–627. [PMC free article] [PubMed] [Google Scholar]
  21. Shillitoe E. J., Lehner T. Immunoglobulins and complement in crevicular fluid, serum and saliva in man. Arch Oral Biol. 1972 Feb;17(2):241–247. doi: 10.1016/0003-9969(72)90206-3. [DOI] [PubMed] [Google Scholar]
  22. Skapski H., Lehner T. A crevicular washing method for investigating immune components of crevicular fluid in man. J Periodontal Res. 1976 Feb;11(1):19–24. doi: 10.1111/j.1600-0765.1976.tb00046.x. [DOI] [PubMed] [Google Scholar]
  23. TOMASI T. B., Jr, TAN E. M., SOLOMON A., PRENDERGAST R. A. CHARACTERISTICS OF AN IMMUNE SYSTEM COMMON TO CERTAIN EXTERNAL SECRETIONS. J Exp Med. 1965 Jan 1;121:101–124. doi: 10.1084/jem.121.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Talbman M. A., Smith D. J. Effects of local immunization with Streptococcus mutans on induction of salivary immunoglobulin A antibody and experimental dental caries in rats. Infect Immun. 1974 Jun;9(6):1079–1091. doi: 10.1128/iai.9.6.1079-1091.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Taubman M. A., Smith D. J. Immune components in dental plaque. J Dent Res. 1976 Apr;55(Spec No):C153–C162. doi: 10.1177/002203457605500309011. [DOI] [PubMed] [Google Scholar]
  26. Theilade E., Wright W. H., Jensen S. B., Löe H. Experimental gingivitis in man. II. A longitudinal clinical and bacteriological investigation. J Periodontal Res. 1966;1:1–13. doi: 10.1111/j.1600-0765.1966.tb01842.x. [DOI] [PubMed] [Google Scholar]
  27. Williams B. D., Challacombe S. J., Slaney J. M., Lachmann P. J., Lehner T. Immunoconglutinins and C3 in human saliva. Clin Exp Immunol. 1975 Mar;19(3):423–433. [PMC free article] [PubMed] [Google Scholar]
  28. Wilton J. M., Renggli H. H., Lehner T. A functional comparison of blood and gingival inflammatory polymorphonuclear leucocytes in man. Clin Exp Immunol. 1977 Jan;27(1):152–158. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES