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. 1978 Aug;33(2):283–291.

Rabbit antiserum to human thymocyte membranes: specificity for normal and malignant T-lymphocytes.

N Gross, C Bron
PMCID: PMC1537555  PMID: 309811

Abstract

An antiserum was obtained by immunization of rabbits with human thymocyte membrane fractions. After appropriate absorptions, the antiserum was shown to detect specifically a population of T-cells. When tested by complement-mediated cytotoxicity the antiserum lysed 95% of thymocytes, 65% of normal PBL and 45% of tonsillar lymphocytes. It was also cytotoxic for three different T-cell lines (MOLT-4, CCRF-CEM and CCRF-HSB-2). When peripheral or tonsillar lymphocytes were separated into populations enriched in B- and T-cells, the percentage of cells lysed by the antiserum correlated well with the proportion of E-rosetting cells. Treatment of PBL with the antiserum and complement resulted in an increase of SmIg-positive B-cells in the residual cell fraction, which could no longer form E-rosettes. Treatment of PBL with the antiserum alone completely inhibited the E-rosette formation. The cytotoxic index on PBL from patients with various lymphoid disorders always correlated with the proportion of T-cells as assessed by E-rosette formation. Finally, the absorptive capacity of thymocytes for the antiserum was ten times higher as compared to that of PBL or tonsil cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bron C., Anckers C., Sauser D. Production of xenogeneic and allogeneic antisera specific for mouse thymus-derived lymphocytes. Eur J Immunol. 1976 Aug;6(8):594–598. doi: 10.1002/eji.1830060814. [DOI] [PubMed] [Google Scholar]
  2. Bron C., Gallagher G. Immunochemical studies of human red cell membrane proteins excreted in normal urine. Immunochemistry. 1974 Aug;11(8):409–415. doi: 10.1016/0019-2791(74)90074-3. [DOI] [PubMed] [Google Scholar]
  3. Brown G., Greaves M. F. Cell surface markers for human T and B lymphocytes. Eur J Immunol. 1974 Apr;4(4):302–310. doi: 10.1002/eji.1830040414. [DOI] [PubMed] [Google Scholar]
  4. Carrel S., Sordat B., Merenda C. Establishment of a cell line (Co-115) from a human colon carcinoma transplanted into nude mice. Cancer Res. 1976 Nov;36(11 Pt 1):3978–3984. [PubMed] [Google Scholar]
  5. Cerottini J. C., Brunner K. T. Localization of mouse isoantigens on the cell surface as revealed by immunofluorescence. Immunology. 1967 Oct;13(4):395–403. [PMC free article] [PubMed] [Google Scholar]
  6. Dean J. H., Silva J. S., McCOY J. L., Leonard C. M., Cannon G. B., Herberman R. B. Functional activities of rosette separated human peripheral blood leukocytes. J Immunol. 1975 Nov;115(5):1449–1455. [PubMed] [Google Scholar]
  7. FOLEY G. E., LAZARUS H., FARBER S., UZMAN B. G., BOONE B. A., MCCARTHY R. E. CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA. Cancer. 1965 Apr;18:522–529. doi: 10.1002/1097-0142(196504)18:4<522::aid-cncr2820180418>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  8. Galili U., Klein E., Schlesinger M. Human T lymphocyte receptors for sheep red blood cells and specific antigens: are they identical sites on the cell membrane? J Immunol. 1977 Jul;119(1):104–109. [PubMed] [Google Scholar]
  9. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  10. Kaplan J., Shope T. C., Peterson W. D., Jr Epstein-barr virus-negative human malignant T-cell lines. J Exp Med. 1974 May 1;139(5):1070–1076. doi: 10.1084/jem.139.5.1070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klein E., Ben-Bassat H., Neumann H., Ralph P., Zeuthen J., Polliack A., Vánky F. Properties of the K562 cell line, derived from a patient with chronic myeloid leukemia. Int J Cancer. 1976 Oct 15;18(4):421–431. doi: 10.1002/ijc.2910180405. [DOI] [PubMed] [Google Scholar]
  12. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 1968 Jul;28(7):1300–1310. [PubMed] [Google Scholar]
  13. Klein G., Giovanella B., Westman A., Stehlin J. S., Mumford D. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology. 1975;5(6):319–334. doi: 10.1159/000149930. [DOI] [PubMed] [Google Scholar]
  14. Klein G., Lindahl T., Jondal M., Leibold W., Menézes J., Nilsson K., Sundström C. Continuous lymphoid cell lines with characteristics of B cells (bone-marrow-derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3283–3286. doi: 10.1073/pnas.71.8.3283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Koyama K., Nakamuro K., Tanigaki N., Pressman D. Alloantigens of human lymphoid cell lines; 'human Ia-like antigens'. Alloantigenic activity and cell line, organ and tissue distribution as determined by radioimmunoassay. Immunology. 1977 Aug;33(2):217–230. [PMC free article] [PubMed] [Google Scholar]
  16. Mann D. L., Abelson L., Henkart P., Harris S. D., Amos D. B. Specific human B lymphocyte alloantigens linked to HL-A. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5103–5106. doi: 10.1073/pnas.72.12.5103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Minowada J., Onuma T., Moore G. E. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst. 1972 Sep;49(3):891–895. [PubMed] [Google Scholar]
  18. Mohanakumar T., Pauly J. L., Sokal J. E., Metzgar R. S. Human leukemia-associated antigens: detection on cells of established lymphoblastoid lines. J Immunol. 1975 Dec;115(6):1542–1548. [PubMed] [Google Scholar]
  19. NISONOFF A., WISSLER F. C., LIPMAN L. N. Properties of the major component of a peptic digest of rabbit antibody. Science. 1960 Dec 9;132(3441):1770–1771. doi: 10.1126/science.132.3441.1770. [DOI] [PubMed] [Google Scholar]
  20. PULVERTAFT J. V. A STUDY OF MALIGNANT TUMOURS IN NIGERIA BY SHORT-TERM TISSUE CULTURE. J Clin Pathol. 1965 May;18:261–273. doi: 10.1136/jcp.18.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reedman B. M., Klein G. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer. 1973 May;11(3):499–520. doi: 10.1002/ijc.2910110302. [DOI] [PubMed] [Google Scholar]
  22. Sauser D., Anckers C., Bron C. Isolation of mouse thymus-derived lymphocyte specific surface antigens. J Immunol. 1974 Aug;113(2):617–624. [PubMed] [Google Scholar]
  23. Snary D., Barnstable C. J., Bodmer W. F., Goodfellow P. N., Crumpton M. J. Cellular distrubtion, purification, and molecular nature of human Ia antigens. Scand J Immunol. 1977;6(5):439–452. doi: 10.1111/j.1365-3083.1977.tb02101.x. [DOI] [PubMed] [Google Scholar]
  24. Takada A., Takada Y., Ito U., Minowada J. Shared antigenic determinants between human brain and human T-cell line. Clin Exp Immunol. 1974 Dec;18(4):491–498. [PMC free article] [PubMed] [Google Scholar]
  25. The T. H., Feltkamp T. E. Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology. 1970 Jun;18(6):865–873. [PMC free article] [PubMed] [Google Scholar]
  26. WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]
  27. Winchester R. J., Wernet P., Kunkel H. G., Dupont B., Jersild C., Fu S. M. Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes. J Exp Med. 1975 Apr 1;141(4):924–929. [PMC free article] [PubMed] [Google Scholar]
  28. Zola H., Mosedale B., Thomas D. The preparation and properties of antisera to subcellular fractions from lymphocytes. Transplantation. 1970 Mar;9(3):259–272. doi: 10.1097/00007890-197003000-00010. [DOI] [PubMed] [Google Scholar]

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