Abstract
The effect of sera from women in different stages of pregnancy on cultures of human lymphocytes stimulated with phytohaemagglutinin (PHA) has been studied and correlated with alpha-foetoprotein (AFP) levels. Sera taken before the twentieth week of gestation showed low levels of AFP and failed to inhibit lymphocyte proliferation. Inhibition was obtained with 38.5% of sera taken from weeks 20 to 30 and with 51.7% of sera after the thirtieth week of pregnancy. Average serum levels of AFP also increased with advanced gestation, but a consistent correlation was not found between AFP concentration and inhibitory activity for every individual serum tested. Lymphocytes from pregnant women or from normal blood donors behaved in the same way in these tests. The mechanisms of inhibition and the role of AFP are discussed.
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- ANDRESEN R. H., MONROE C. W. Experimental study of the behavior of adult human skin homografts during pregnancy. A preliminary report. Am J Obstet Gynecol. 1962 Oct 15;84:1096–1103. doi: 10.1016/0002-9378(62)90560-4. [DOI] [PubMed] [Google Scholar]
- Carr M. C., Stites D. P., Fudenberg H. H. Cellular immune aspects of the human fetal maternal relationship. II. In vitro response of gravida lymphocytes to phytohemagglutinin. Cell Immunol. 1973 Sep;8(3):448–454. doi: 10.1016/0008-8749(73)90136-6. [DOI] [PubMed] [Google Scholar]
- Charpentier B., Guttmann R. D., Shuster J., Gold P. Augmentation of proliferation of human mixed lymphocyte culture by human alpha-fetoprotein. J Immunol. 1977 Sep;119(3):897–900. [PubMed] [Google Scholar]
- Farber P. A., Glasgow L. A. Factors Modifying Host Resistance to Virus Infection: II. Enhanced Susceptibility of Mice to Encephalomyocarditis Virus Infection During Pregnancy. Am J Pathol. 1968 Sep;53(3):463–481. [PMC free article] [PubMed] [Google Scholar]
- Hsu C. C. Peripheral blood lymphocyte responses to phytohemagglutinin and pokeweed mitogen during pregnancy. Proc Soc Exp Biol Med. 1974 Jul;146(3):771–775. doi: 10.3181/00379727-146-38190. [DOI] [PubMed] [Google Scholar]
- Johansson S. G., Kjessler B., Sherman M. S. Determination of alpha-fetoprotein by a new paper disc radioimmunoassay of the sandwich type. Int Arch Allergy Appl Immunol. 1976;52(1-4):384–391. doi: 10.1159/000231705. [DOI] [PubMed] [Google Scholar]
- Murgita R. A. The immunosuppressive role of alpha-fetoprotein during pregnancy. Scand J Immunol. 1976;5(9):1003–1014. doi: 10.1111/j.1365-3083.1976.tb03052.x. [DOI] [PubMed] [Google Scholar]
- Murgita R. A., Tomasi T. B., Jr Suppression of the immune response by alpha-fetoprotein on the primary and secondary antibody response. J Exp Med. 1975 Feb 1;141(2):269–286. doi: 10.1084/jem.141.2.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Purtilo D. T., Hallgren H. M., Yunis E. J. Depressed maternal lymphocyte response to phytohaemagglutinin in human pregnancy. Lancet. 1972 Apr 8;1(7754):769–771. doi: 10.1016/s0140-6736(72)90522-3. [DOI] [PubMed] [Google Scholar]
- Uriel J., de Nechaud B., Dupiers M. Estrogen-binding properties of rat, mouse and man fetospecific serum proteins. Demonstration by immuno-autoradiographic methods. Biochem Biophys Res Commun. 1972 Feb 16;46(3):1175–1180. doi: 10.1016/s0006-291x(72)80098-6. [DOI] [PubMed] [Google Scholar]
- Yachnin S. Demonstration of the inhibitory effect of human alpha-fetoprotein on in vitro transformation of human lymphocytes. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2857–2861. doi: 10.1073/pnas.73.8.2857. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yachnin S., Lester E. Inhibition of human lymphocyte transformation by human alpha-foetoprotein (HAFP); comparison of foetal and hepatoma HAFP and kinetic studies in vitro immunosuppression. Clin Exp Immunol. 1976 Dec;26(3):484–490. [PMC free article] [PubMed] [Google Scholar]